KU BIOL 600 TEST 4 UPDATED ACTUAL
QUESTIONS AND CORRECT ANSWERS
Question:
1. The formation of two acetyl CoA from two molecules of pyruvate will generate ____ moleucles of
CO2 and ____ molecules of NADH.
Answer:
2, 2
Question:
2. The pyruvate dehydrogenase component catalyzes the 1.______. It uses 2.____ as a prostheticgroup
and
3.___ as a stoichiometric coenzyme.
Answer:
1. oxidative decarboxylation of pyruvate
2. TPP
3. N/A
Question:
3. The dihydrolipoyl transacetylase catalyzes the
1.______. It uses 2.______ as a prosthetic group and
3.______ as a stoichiometric coenzyme
Answer:
1. transfer of an acetyl group to Coenzyme A
2. Lipoamide
3. Coenzyme A
Question:
4. The dihydrolipoyl dehydrogenase catalyzes the
1.______. It uses 2.______ as a prosthetic group and
3._____ as a stoichiometric coenzyme
Answer:
1. regeneration of lipoamide
2. FAD
3. NAD+
Question:
5. what is the rate limiting step in the pyruvate dehydrogenase complex
Answer:
E1
Question:
6. name of E1 enzyme
Answer:
pyruvate dehydrogenase complex
,Question:
7. name of e2 enzyme
Answer:
dihydrolipoyl transacetylase
Question:
8. name of e3 enzyme
Answer:
dihydrolipoyl dehydrogenase
Question:
9. first step of acetyl CoA synthesis
Answer:
decarboxylation of pyruvate
Question:
10. second step of acetyl coA synthesis
Answer:
oxidation and transfer to lipoamide
Question:
11. step 3 of acetyl coA synthesis
Answer:
acetyl group transferred to CoA
Question:
12. what has to be re-oxidized for acetyl coA synthesis to continue
Answer:
dihydrolipoamide
Question:
13. what does dihydrolipoamide oxidize to form
Answer:
lipoamide
Question:
14. what molecules are inhibitors of the PDH complex
Answer:
NADH, ATP, acetyl coA
Question:
15. what molecules are activators of the PDH complex
Answer:
ADP, pyruvate
Question:
16. what is being allosterically regulated in the PDH complex
, Answer:
kinase
Question:
17. what does adding a phosphoric group do to the PDH complex
Answer:
inactivated it
Question:
18. what does the removal of a phosphoric group do to the PDH complex
Answer:
activates it
Question:
19. what adds a phosphoric group to PDH complex
Answer:
kinase
Question:
20. what removes the phosphoric group from PDH complex
Answer:
phosphatase
Question:
21. what energy level causes the activation of PDH complex
Answer:
low energy
Question:
22. what energy level causes the inactivation of PDH complex
Answer:
high energy
Question:
23. for every molecule of acetyl coA ___ ATP molecules, _____ NADH molecules, and ____ FADH2
are released
Answer:
1 ATP 3NADH 1 FADH2
Question:
24. Per molecule of glucose, how much ATP, NADH, and FADH2 is produced in the citric acid cycle?
Answer:
2 ATP 6 NADH 2 FADH2
Question:
25. Enzyme: Citrate Synthase substrate: ______ product: ______
Answer:
substrate: Oxaloacetate and Acetyl CoA product: citrate
QUESTIONS AND CORRECT ANSWERS
Question:
1. The formation of two acetyl CoA from two molecules of pyruvate will generate ____ moleucles of
CO2 and ____ molecules of NADH.
Answer:
2, 2
Question:
2. The pyruvate dehydrogenase component catalyzes the 1.______. It uses 2.____ as a prostheticgroup
and
3.___ as a stoichiometric coenzyme.
Answer:
1. oxidative decarboxylation of pyruvate
2. TPP
3. N/A
Question:
3. The dihydrolipoyl transacetylase catalyzes the
1.______. It uses 2.______ as a prosthetic group and
3.______ as a stoichiometric coenzyme
Answer:
1. transfer of an acetyl group to Coenzyme A
2. Lipoamide
3. Coenzyme A
Question:
4. The dihydrolipoyl dehydrogenase catalyzes the
1.______. It uses 2.______ as a prosthetic group and
3._____ as a stoichiometric coenzyme
Answer:
1. regeneration of lipoamide
2. FAD
3. NAD+
Question:
5. what is the rate limiting step in the pyruvate dehydrogenase complex
Answer:
E1
Question:
6. name of E1 enzyme
Answer:
pyruvate dehydrogenase complex
,Question:
7. name of e2 enzyme
Answer:
dihydrolipoyl transacetylase
Question:
8. name of e3 enzyme
Answer:
dihydrolipoyl dehydrogenase
Question:
9. first step of acetyl CoA synthesis
Answer:
decarboxylation of pyruvate
Question:
10. second step of acetyl coA synthesis
Answer:
oxidation and transfer to lipoamide
Question:
11. step 3 of acetyl coA synthesis
Answer:
acetyl group transferred to CoA
Question:
12. what has to be re-oxidized for acetyl coA synthesis to continue
Answer:
dihydrolipoamide
Question:
13. what does dihydrolipoamide oxidize to form
Answer:
lipoamide
Question:
14. what molecules are inhibitors of the PDH complex
Answer:
NADH, ATP, acetyl coA
Question:
15. what molecules are activators of the PDH complex
Answer:
ADP, pyruvate
Question:
16. what is being allosterically regulated in the PDH complex
, Answer:
kinase
Question:
17. what does adding a phosphoric group do to the PDH complex
Answer:
inactivated it
Question:
18. what does the removal of a phosphoric group do to the PDH complex
Answer:
activates it
Question:
19. what adds a phosphoric group to PDH complex
Answer:
kinase
Question:
20. what removes the phosphoric group from PDH complex
Answer:
phosphatase
Question:
21. what energy level causes the activation of PDH complex
Answer:
low energy
Question:
22. what energy level causes the inactivation of PDH complex
Answer:
high energy
Question:
23. for every molecule of acetyl coA ___ ATP molecules, _____ NADH molecules, and ____ FADH2
are released
Answer:
1 ATP 3NADH 1 FADH2
Question:
24. Per molecule of glucose, how much ATP, NADH, and FADH2 is produced in the citric acid cycle?
Answer:
2 ATP 6 NADH 2 FADH2
Question:
25. Enzyme: Citrate Synthase substrate: ______ product: ______
Answer:
substrate: Oxaloacetate and Acetyl CoA product: citrate