JERSEY COLLEGE BIO 201
MICROBIOLOGY FINAL EXAM
2026/2027: 50 VERIFIED QUESTIONS &
ANSWERS FOR NURSING & ALLIED
HEALTH PREP
Section I: History and Fundamentals of Microbiology
1. Which scientist first observed unicellular organisms, which he called
"animalcules," using a microscope he developed?
o A) Robert Koch
o B) Louis Pasteur
o C) Antonie van Leeuwenhoek
o D) Robert Hooke
o Correct answer: C. Antonie van Leeuwenhoek. He was the first to
observe and describe microorganisms, which he referred to as
"animalcules," using a simple microscope he developed.
2. Louis Pasteur's famous swan-neck flask experiment disproved which
theory?
o A) Germ theory of disease
o B) Cell theory
o C) Spontaneous generation
o D) Biogenesis
o Correct answer: C. Spontaneous generation. His experiment
demonstrated that broth remained sterile when exposed to air because
dust and microbes were trapped in the curved neck, definitively
disproving the theory that living organisms could arise from nonliving
matter.
, 3. Who developed the first solid media culture techniques using agar as a
solidifying agent?
o A) Richard Petri
o B) Robert Koch
o C) Alexander Fleming
o D) Louis Pasteur
o Correct answer: B. Robert Koch. He developed solid media culture
techniques using agar, which were foundational to microbiology.
4. Koch's postulates state all of the following EXCEPT:
o A) The microbe must be present in every animal with the disease.
o B) The microbe can be isolated and grown in pure culture.
o C) The cultured microorganism must cause the same disease in an
inoculated healthy animal.
o D) The microbe must be treatable with antibiotics.
o Correct answer: D. The microbe must be treatable with antibiotics.
Treatability is not part of Koch's postulates, which are used to establish
a causal relationship between a microbe and a disease.
5. Which of the following is NOT a microorganism?
o A) Bacterium
o B) Fungus
o C) Insect
o D) Protozoan
o Correct answer: C. Insect. Insects are macroscopic organisms and are
not classified as microorganisms.
6. The taxonomic resource for bacteria is the:
o A) Bergen's Manual
o B) Bergey's Manual
o C) Bacteriological Code
o D) International Code of Nomenclature
o Correct answer: B. Bergey's Manual. Bergey's Manual of Systematic
Bacteriology is the primary taxonomic resource for classifying and
identifying bacteria.
MICROBIOLOGY FINAL EXAM
2026/2027: 50 VERIFIED QUESTIONS &
ANSWERS FOR NURSING & ALLIED
HEALTH PREP
Section I: History and Fundamentals of Microbiology
1. Which scientist first observed unicellular organisms, which he called
"animalcules," using a microscope he developed?
o A) Robert Koch
o B) Louis Pasteur
o C) Antonie van Leeuwenhoek
o D) Robert Hooke
o Correct answer: C. Antonie van Leeuwenhoek. He was the first to
observe and describe microorganisms, which he referred to as
"animalcules," using a simple microscope he developed.
2. Louis Pasteur's famous swan-neck flask experiment disproved which
theory?
o A) Germ theory of disease
o B) Cell theory
o C) Spontaneous generation
o D) Biogenesis
o Correct answer: C. Spontaneous generation. His experiment
demonstrated that broth remained sterile when exposed to air because
dust and microbes were trapped in the curved neck, definitively
disproving the theory that living organisms could arise from nonliving
matter.
, 3. Who developed the first solid media culture techniques using agar as a
solidifying agent?
o A) Richard Petri
o B) Robert Koch
o C) Alexander Fleming
o D) Louis Pasteur
o Correct answer: B. Robert Koch. He developed solid media culture
techniques using agar, which were foundational to microbiology.
4. Koch's postulates state all of the following EXCEPT:
o A) The microbe must be present in every animal with the disease.
o B) The microbe can be isolated and grown in pure culture.
o C) The cultured microorganism must cause the same disease in an
inoculated healthy animal.
o D) The microbe must be treatable with antibiotics.
o Correct answer: D. The microbe must be treatable with antibiotics.
Treatability is not part of Koch's postulates, which are used to establish
a causal relationship between a microbe and a disease.
5. Which of the following is NOT a microorganism?
o A) Bacterium
o B) Fungus
o C) Insect
o D) Protozoan
o Correct answer: C. Insect. Insects are macroscopic organisms and are
not classified as microorganisms.
6. The taxonomic resource for bacteria is the:
o A) Bergen's Manual
o B) Bergey's Manual
o C) Bacteriological Code
o D) International Code of Nomenclature
o Correct answer: B. Bergey's Manual. Bergey's Manual of Systematic
Bacteriology is the primary taxonomic resource for classifying and
identifying bacteria.
