BIO 325 Genetics Problem Set 5 Questions and Answers 2026/27 Latest - UT Austin
1. Suppose you want to amplify the region in gray from the two ends of this
DNA sequence. What would your 12 base primers be
GGA 5’
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCT 3’
3’ ACGAAAGGCTT
TGCTTTCCGGAA->
5’TGCTTTCCGGAA
5’AGGTTGGCAATA
2. Two wild-type fragments of human genomic DNA from the long arms of two
nonhomologous acrocentric chromosomes are shown in
the accompanying diagram. On both fragments, the centromere-to-telomere direction is left-to-
right. The red lines numbered I, and 2 indicate the positions of breakpoints that could be caused
by ionizing radiation. Certain combinations of these breaks could produce chromosomal
rearrangements when the broken pieces are put back together by DNA repair systems.
You will want to diagnose the presence of the rearrangements by using PCR In every case that
follows, one of the primers you will use for PCR is primer A, whose sequence is:
Primer A 5’ TGCTTTCCGGAAG 3’
2
1 5’
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGGAATATCCATGGGCACC3’
3’ AGCTAAGGCCTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCCTTATAGGTACCCGTGG
5’ TCGATT =>
Answer 5’ GGTGCCCATGGATATT 3’
Assume a breakpoint between 1&2
Write the sequence and polarity of a 16-base primer that, in conjunction with
primer A, will allow you to diagnose the presence of this deletion in a
person's genomic DNA
Write the sequence and polarity of a 16-base primer that, in conjunct ion
with primer A, will allow you to diagnose the presence of
this inversion.
.
OR
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGGAATATCCATGGGCACC3’
3’ AGCTAAGGCCTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCCTTATAGGTACCCGTGG
5' TAGTTTCCCGGGACGT
BIO 325 Genetics Problem Set 5
, 3. Below are a series of genes on a chromosome. How could you duplicate gene C without
structurally damaging (breaking) the chromosome. Unequal crossover
A B C D E
A B C D E
4. Suppose we have two homologous chromosomes where one has an inversion. The centrosome
is shown as the black circle. What are the chromosomes that result from a crossover between C
and D?
A B C D E
A D C B E
5. Suppose we have two homologous chromosomes where one has an inversion. The centrosome
is shown as the black circle. What are the chromosomes that result from a crossover between C
and D?
A B C D E
BIO 325 Genetics Problem Set 5
1. Suppose you want to amplify the region in gray from the two ends of this
DNA sequence. What would your 12 base primers be
GGA 5’
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCT 3’
3’ ACGAAAGGCTT
TGCTTTCCGGAA->
5’TGCTTTCCGGAA
5’AGGTTGGCAATA
2. Two wild-type fragments of human genomic DNA from the long arms of two
nonhomologous acrocentric chromosomes are shown in
the accompanying diagram. On both fragments, the centromere-to-telomere direction is left-to-
right. The red lines numbered I, and 2 indicate the positions of breakpoints that could be caused
by ionizing radiation. Certain combinations of these breaks could produce chromosomal
rearrangements when the broken pieces are put back together by DNA repair systems.
You will want to diagnose the presence of the rearrangements by using PCR In every case that
follows, one of the primers you will use for PCR is primer A, whose sequence is:
Primer A 5’ TGCTTTCCGGAAG 3’
2
1 5’
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGGAATATCCATGGGCACC3’
3’ AGCTAAGGCCTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCCTTATAGGTACCCGTGG
5’ TCGATT =>
Answer 5’ GGTGCCCATGGATATT 3’
Assume a breakpoint between 1&2
Write the sequence and polarity of a 16-base primer that, in conjunction with
primer A, will allow you to diagnose the presence of this deletion in a
person's genomic DNA
Write the sequence and polarity of a 16-base primer that, in conjunct ion
with primer A, will allow you to diagnose the presence of
this inversion.
.
OR
5’ TGCTTTCCGGAAGCTTAGTTTCCCGGGACGTATTGCCAACCTAGGTAAGCGCCGGAATATCCATGGGCACC3’
3’ AGCTAAGGCCTTCGAATCAAAGGGCCCTGCATAACGGTTGGATCCATTCGCGGCCTTATAGGTACCCGTGG
5' TAGTTTCCCGGGACGT
BIO 325 Genetics Problem Set 5
, 3. Below are a series of genes on a chromosome. How could you duplicate gene C without
structurally damaging (breaking) the chromosome. Unequal crossover
A B C D E
A B C D E
4. Suppose we have two homologous chromosomes where one has an inversion. The centrosome
is shown as the black circle. What are the chromosomes that result from a crossover between C
and D?
A B C D E
A D C B E
5. Suppose we have two homologous chromosomes where one has an inversion. The centrosome
is shown as the black circle. What are the chromosomes that result from a crossover between C
and D?
A B C D E
BIO 325 Genetics Problem Set 5
