BIO 325 Midterm Exam 3 Questions and 100% Correct Answers
2026/27 Update - UT Austin
• No questions will be answered during the exam.
Figuring out what the questions mean is part of the exam.
• Be sure to circle your answers.
If nothing is circled, you won’t get credit.
Reason: I will not pick the correct answer from among multiple answers.
• NO CALCULATORS.
Leave numerical answers as expressions (e.g. 1/2 × 1/3 instead of 1/6).
Do not do the arithmetic.
It wastes your time, and if you make an error, it will count against you.
• You must show how you got your answer in the space provided – not on your
scratch paper. If all you have on your page is a number or a word, there is no way for
me to know if you guessed, copied it, or figured it out in your head. Credit is given only
for knowing how to figure out the answer – so you need to tell me how you did that.
• The entire exam contains 100 points.
Tips:
• Answer the question asked, not a different one that you prefer. (zero points for that)
• Do not supply information not asked for. That will count against you if it suggests
that you didn’t understand the question and that you’re throwing spaghetti against the
wall, or if the information is incorrect.
BIO 325 Midterm Exam 3
, 1. Suppose you need to analyze the probability that 3 children of young parents where
Huntington’s disease is in the family. Furthermore suppose the sequence below is the target
region bracketing SSR repeats DNA sequence. 10 pts
5' GGCTAAGATCTGAATTTTCCGAG [SSR] TTGGGCAATAATGTAGCGCCTT 3'
3' CCGATTCTAGACTTAAAAGGCTC [SSR] AACCCGTTATTACATCGCGGAA 5
1A. find primer pairs (below) that will amplify the DNA containing the SSRs
A. 5' GGAAAATTCAGATCTTAG 3';
5' TGGGCAATAATGTAGCGC 3'
B. 3' GATTCTAGACTTAAAGGC 5';
3' ACCCGTTATTACATCGCG 5'
C. 5' GCTAAGATCTGAATTTTC 3';
3' ACCCGTTATTACATCGCG 5'
D. 5' GCTAAGATCTGAATTTTC 3';
5' TGGGCAATAATGTAGCGC 3'
1B Suppose that among the 3 children one is normal, one is preHuntington’s and one has early
onset disease. Show on a gel in different lanes what each of the PCR products would look
like.5pts
Early onset
PreHuntington’s
Normal
15pts
BIO 325 Midterm Exam 3
2026/27 Update - UT Austin
• No questions will be answered during the exam.
Figuring out what the questions mean is part of the exam.
• Be sure to circle your answers.
If nothing is circled, you won’t get credit.
Reason: I will not pick the correct answer from among multiple answers.
• NO CALCULATORS.
Leave numerical answers as expressions (e.g. 1/2 × 1/3 instead of 1/6).
Do not do the arithmetic.
It wastes your time, and if you make an error, it will count against you.
• You must show how you got your answer in the space provided – not on your
scratch paper. If all you have on your page is a number or a word, there is no way for
me to know if you guessed, copied it, or figured it out in your head. Credit is given only
for knowing how to figure out the answer – so you need to tell me how you did that.
• The entire exam contains 100 points.
Tips:
• Answer the question asked, not a different one that you prefer. (zero points for that)
• Do not supply information not asked for. That will count against you if it suggests
that you didn’t understand the question and that you’re throwing spaghetti against the
wall, or if the information is incorrect.
BIO 325 Midterm Exam 3
, 1. Suppose you need to analyze the probability that 3 children of young parents where
Huntington’s disease is in the family. Furthermore suppose the sequence below is the target
region bracketing SSR repeats DNA sequence. 10 pts
5' GGCTAAGATCTGAATTTTCCGAG [SSR] TTGGGCAATAATGTAGCGCCTT 3'
3' CCGATTCTAGACTTAAAAGGCTC [SSR] AACCCGTTATTACATCGCGGAA 5
1A. find primer pairs (below) that will amplify the DNA containing the SSRs
A. 5' GGAAAATTCAGATCTTAG 3';
5' TGGGCAATAATGTAGCGC 3'
B. 3' GATTCTAGACTTAAAGGC 5';
3' ACCCGTTATTACATCGCG 5'
C. 5' GCTAAGATCTGAATTTTC 3';
3' ACCCGTTATTACATCGCG 5'
D. 5' GCTAAGATCTGAATTTTC 3';
5' TGGGCAATAATGTAGCGC 3'
1B Suppose that among the 3 children one is normal, one is preHuntington’s and one has early
onset disease. Show on a gel in different lanes what each of the PCR products would look
like.5pts
Early onset
PreHuntington’s
Normal
15pts
BIO 325 Midterm Exam 3
