PNB 2265 LAB 4 EXAM QUESTIONS
AND ANSWERS
respiratory pigments - Correct Answers -proteins that transport oxygen, greatly increase
the amount of oxygen that blood can carry
what is the respiratory pigment in vertebrates? - Correct Answers -hemoglobin
oxyhemoglobin dissociation curve - Correct Answers -Relationship between available
oxygen and amount of oxygen carried by hemoglobin.
sigmoidal shape
binding of oxygen to hemoglobin dependent upon partial pressure of oxygen
cooperative binding of hemoglobin - Correct Answers -- hemoglobin consists of four
prosthetic heme groups that can bind one O2 each, as each individual O2 molecule
binds, it increases the affinity of the next heme group for the additional O2 molecule
- also works in reverse for O2 offloading
- results in a sigmoidal dissociation curve
which value is C in saturation equation? - Correct Answers -the sample at 760mmHg,
no oxygen taken out
absorbance measured at 660nm
Absorbance at 660nm after each pressure reduction
which value is B in saturation equation? - Correct Answers -air pressure has been
reduced from vacuum monometer
Absorbance at 660nm after each pressure reduction
upper pleateau of sigmoid curve - Correct Answers -Hb remains almost fully saturated
in light of any respiratory/circulatory abnormalities as is typically experienced in old age,
illness, or a low oxygen environment (e.g. high altitude).
p50 - Correct Answers -half-saturation pressure
at this point, half of hemoglobin present as oxyhemoglobin
Right Shift Curve - Correct Answers -P50 increases
lower affinity for oxygen
promotes release of oxygen
Left Shift Curve - Correct Answers -p50 decreases
higher affinity for oxygen
promotes the loading of oxygen
AND ANSWERS
respiratory pigments - Correct Answers -proteins that transport oxygen, greatly increase
the amount of oxygen that blood can carry
what is the respiratory pigment in vertebrates? - Correct Answers -hemoglobin
oxyhemoglobin dissociation curve - Correct Answers -Relationship between available
oxygen and amount of oxygen carried by hemoglobin.
sigmoidal shape
binding of oxygen to hemoglobin dependent upon partial pressure of oxygen
cooperative binding of hemoglobin - Correct Answers -- hemoglobin consists of four
prosthetic heme groups that can bind one O2 each, as each individual O2 molecule
binds, it increases the affinity of the next heme group for the additional O2 molecule
- also works in reverse for O2 offloading
- results in a sigmoidal dissociation curve
which value is C in saturation equation? - Correct Answers -the sample at 760mmHg,
no oxygen taken out
absorbance measured at 660nm
Absorbance at 660nm after each pressure reduction
which value is B in saturation equation? - Correct Answers -air pressure has been
reduced from vacuum monometer
Absorbance at 660nm after each pressure reduction
upper pleateau of sigmoid curve - Correct Answers -Hb remains almost fully saturated
in light of any respiratory/circulatory abnormalities as is typically experienced in old age,
illness, or a low oxygen environment (e.g. high altitude).
p50 - Correct Answers -half-saturation pressure
at this point, half of hemoglobin present as oxyhemoglobin
Right Shift Curve - Correct Answers -P50 increases
lower affinity for oxygen
promotes release of oxygen
Left Shift Curve - Correct Answers -p50 decreases
higher affinity for oxygen
promotes the loading of oxygen
