PCB 3023L Final Review | UPDATED Questions with 100% Verified Answers

PCB 3023L Final Review | UPDATED Questions with 100% Verified
Answers

Question:
What does SDS PAGE show us regarding the recombinant
protein sample?

Answer:
protein size (weight) and purity


Question:
What does Western Blot show with regard to the recombinant
protein sample?

Answer:
detection of specific protein


Question:
What is the "transfer sandwich" composed of?

Answer:
2 scotchbrite pads, 4 pieces of whatman paper, nitrocellulose filter, gel.
All encased within a tranfer clamp (black side - clear side +)


Question:
What are the 5 samples?

Answer:
Desalted, Eluate, Wash, Unbound, Soluble


Question:
What does SDS-PAGE stand for?

Answer:
sodium dodecyl sulfate polyacrylamide gel electrophoresis


Question:
How many amino acids are proteins composed of?

, Answer:
20


Question:
1 Dalton =

Answer:
1 hydrogen atom
This is used to measure weight of proteins


Question:
How is the electric charge of proteins accounted for?

Answer:
Masked by a strongly negative detergent, the SDS.


Question:
Why are the proteins denatured?

Answer:
This is carried out through heat and SDS, this is so proteins migrate through the gel with
just mass as the variable.


Question:
What factors denature a protein?

Answer:
Heat, detergent, reducing agent


Question:
Lower part of chamber contains

Answer:
the anode (+)


Question:
Upper part of chamber contains

Answer: