ACS Biochemistry UPDATED ACTUAL Exam Questions and
CORRECT Answers | Verified | 100% Accurate
Question 1.
thioester bond (1)
Correct Answer: compounds with functional group C-S-CoA-C (eg, in
acetyl-CoA)
Question 2.
zwitterion (3)
Correct Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
Question 3.
Isoelectric point (pI) (chapter 3)
Correct Answer: the characteristic pH at which the net electric charge is zero
Question 4.
SDS - Sodium Dodecyl Sulfate (chap 3)
Correct Answer: detergent used to unfold proteins and give them uniform
negative charge
Question 5.
SDS Page (3)
Correct Answer: chromatography used to separate proteins based on mass.
light proteins travel fast than heavier ones
Question 6.
PCR - Polymerase chain reaction (3)
Correct Answer: copies DNA multiple times to increase sample size
Question 7.
Isoelectric focusing (chapt 3)
Correct Answer: procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)
, Question 8.
Two-Dimensional Electrophoresis (3)
Correct Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
Question 9.
specific activity (3)
Correct Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
Question 10.
activity (3)
Correct Answer: total units of a certain enzyme in a solution
Question 11.
Peptide bonds (chapter 4)
Correct Answer: C-N bond with double bond character due to resonance (C-N
bond cannot rotate, and is planar)
• Edman degredation (3) -✓✓used in the sequencing of polypeptides; labels
and removes ONLY the amino-residue from a polypeptide. carried out in a
machine called a sequenator
Question 12.
φ in peptide bonding (chapter 4)
Correct Answer: angle around the α-carbon - amide nitrogen bond
Question 13.
ψ in peptide bonding (chapter 4)
Correct Answer: angle around the α-carbon - carbonyl carbon bond
Question 14.
Ramachandran Plot (4)
Correct Answer: shows favoreable φ-ψ angle combinations. 3 main "wells" for
α-helices, β-sheets, and left handed α-helices
Question 15.
Levinthal's Paradox (4)
CORRECT Answers | Verified | 100% Accurate
Question 1.
thioester bond (1)
Correct Answer: compounds with functional group C-S-CoA-C (eg, in
acetyl-CoA)
Question 2.
zwitterion (3)
Correct Answer: dipolar ion with both positive and negative groups but overall
neutral charge; can act as either an acid or base
Question 3.
Isoelectric point (pI) (chapter 3)
Correct Answer: the characteristic pH at which the net electric charge is zero
Question 4.
SDS - Sodium Dodecyl Sulfate (chap 3)
Correct Answer: detergent used to unfold proteins and give them uniform
negative charge
Question 5.
SDS Page (3)
Correct Answer: chromatography used to separate proteins based on mass.
light proteins travel fast than heavier ones
Question 6.
PCR - Polymerase chain reaction (3)
Correct Answer: copies DNA multiple times to increase sample size
Question 7.
Isoelectric focusing (chapt 3)
Correct Answer: procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)
, Question 8.
Two-Dimensional Electrophoresis (3)
Correct Answer: combines isoelectric focusing and SDS electrophoresis;
separates proteins by both molecular weight and pI
Question 9.
specific activity (3)
Correct Answer: number of enzyme units per mg of total protein (a measure of
enzyme purity)
Question 10.
activity (3)
Correct Answer: total units of a certain enzyme in a solution
Question 11.
Peptide bonds (chapter 4)
Correct Answer: C-N bond with double bond character due to resonance (C-N
bond cannot rotate, and is planar)
• Edman degredation (3) -✓✓used in the sequencing of polypeptides; labels
and removes ONLY the amino-residue from a polypeptide. carried out in a
machine called a sequenator
Question 12.
φ in peptide bonding (chapter 4)
Correct Answer: angle around the α-carbon - amide nitrogen bond
Question 13.
ψ in peptide bonding (chapter 4)
Correct Answer: angle around the α-carbon - carbonyl carbon bond
Question 14.
Ramachandran Plot (4)
Correct Answer: shows favoreable φ-ψ angle combinations. 3 main "wells" for
α-helices, β-sheets, and left handed α-helices
Question 15.
Levinthal's Paradox (4)
