BLD 313 exam3
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. A cuvette of absorption spectrophotometry can be made of polystyrene (plastic), glass,
quartz, etc. How do we choose the type of Corvette? - Correct Answer: It is based on
the wavelength we need to detect.
2. Which of the following can be done to increase the specificity as well as the sensitivity of
spectrophotometric analysis? - Correct Answer: Narrow the band pass
3. Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis? - Correct Answer: Increased
specificity and increased sensitivity
4. Flurometers are designed so that the path of the exciting light is at a right angle to the
path of the emitted light. What is the purpose of this design? - Correct Answer:
Prevent excitation light from reaching the detector
5. Which of the following is measured in time-delayed fluorescence? - Correct Answer:
Phosphorescence
6. Which of the following statements is true about fluorescent polarization? - Correct
Answer: It can be used in competitive-binding assay.
, 7. Which of the following techniques detects unabsorbed, reflected light by a
photodetector? - Correct Answer: Reflectance photometry
8. Which of the following is used for urine dip stick reading? - Correct Answer:
Reflectance photometry
9. Which of the following is measured in turbidimetry? - Correct Answer: Reduction of
transmitted light due to scatter
10. Which of the following is measured in nephelometry? - Correct Answer: Scattered
light
11. Which of the following statement is true about nephelometry? - Correct Answer:
The concentration to be measured is directly proportional to scattered light.
12. Which of the following is associated with chemiluminescence? - Correct Answer: The
chemical reaction produces an intermediate compound (excited electron), which
becomes stable final product (ground state electron). A light is emitted when the excited
electron goes back to the ground state.
13. Which of the following is associated with a bioluminescence? - Correct Answer: The
enzyme reaction produces an intermediate compound (excited electron), which
becomes stable final product (ground state electron). A light is emitted when the excited
electron goes back to the ground state.
14. Luminescence is caused by which of the following? - Correct Answer: Photons
emitted while excited electrons drop down to the ground state
Comprehensive Resource To Help You Ace 2026-2027 Exams
Includes Frequently Tested Questions With ELABORATED
100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
1. A cuvette of absorption spectrophotometry can be made of polystyrene (plastic), glass,
quartz, etc. How do we choose the type of Corvette? - Correct Answer: It is based on
the wavelength we need to detect.
2. Which of the following can be done to increase the specificity as well as the sensitivity of
spectrophotometric analysis? - Correct Answer: Narrow the band pass
3. Which of the following represents a primary advantage of performing fluorometric over
absorption spectroscopic methods of analysis? - Correct Answer: Increased
specificity and increased sensitivity
4. Flurometers are designed so that the path of the exciting light is at a right angle to the
path of the emitted light. What is the purpose of this design? - Correct Answer:
Prevent excitation light from reaching the detector
5. Which of the following is measured in time-delayed fluorescence? - Correct Answer:
Phosphorescence
6. Which of the following statements is true about fluorescent polarization? - Correct
Answer: It can be used in competitive-binding assay.
, 7. Which of the following techniques detects unabsorbed, reflected light by a
photodetector? - Correct Answer: Reflectance photometry
8. Which of the following is used for urine dip stick reading? - Correct Answer:
Reflectance photometry
9. Which of the following is measured in turbidimetry? - Correct Answer: Reduction of
transmitted light due to scatter
10. Which of the following is measured in nephelometry? - Correct Answer: Scattered
light
11. Which of the following statement is true about nephelometry? - Correct Answer:
The concentration to be measured is directly proportional to scattered light.
12. Which of the following is associated with chemiluminescence? - Correct Answer: The
chemical reaction produces an intermediate compound (excited electron), which
becomes stable final product (ground state electron). A light is emitted when the excited
electron goes back to the ground state.
13. Which of the following is associated with a bioluminescence? - Correct Answer: The
enzyme reaction produces an intermediate compound (excited electron), which
becomes stable final product (ground state electron). A light is emitted when the excited
electron goes back to the ground state.
14. Luminescence is caused by which of the following? - Correct Answer: Photons
emitted while excited electrons drop down to the ground state
