BIOCHEM ACS FINAL STUDY GUIDE | Actual Questions and
Answers Latest Updated 2026/2027 (Graded A+)
• Question
Which Amino Acids will be charged at pH 7?
Correct Answer: DEHRK: Aspartic Acid (Asp), Glutamic Acid (Glu), Histidine (His),
Arginine (Arg), Lysine (Lys)
• Question
Which amino acids will have a negative charge at pH 7?
Correct Answer: D&E: Aspartic Acid (Asp) and Glutamic acid (Glu)
• Question
Which amino acids will have a positive charge at pH 7?
Correct Answer: H, R, K: Histidine (His), Arginine (Arg), Lysine (Lys)
• Question
Which of the following sequences are most likely to be in the interior core of a globular
protein?
(A) ITFWLI (B) IKFGVA (C) DKYPGE (D) MPCDNA
Correct Answer: The interior is hydrophilic so sequence: ITFWLI is most likely to be in the
interior because there is no charged amino acids and had the lowest percentage of polar
amino acids.
• Question
How are beta sheets stabilized in proteins?
Correct Answer: Hydrogen bonding between peptide backbone groups.
• Question
Fibrous Proteins
Correct Answer: Not water soluble, not flexible in secondary structure, highly repetitive
primary structure, elongated structure; 2 α keratin polypeptides each which forms an α
helix twisting around each other to form a left handed coil.
• Question
Globular Proteins
Correct Answer: Water soluble, flexible in secondary structure, not necessarily highly
repetitive, size and ability to associate into a larger structure varies.
, • Question
Gel filtration elution
Correct Answer: Largest elutes first
• Question
Competitive inhibitor
Correct Answer: Vmax stays the same different km
• Question
Noncompetitive inhibitor
Correct Answer: Same X axis on reciprocal, same Km different vmax
• Question
Uncompetitive inhibitor
Correct Answer: Different Km and vmax
• Question
Catalytic efficiency
Correct Answer: Kcat/km higher is more efficient
• Question
What are the structural changes in hemoglobin that allows for cooperative binding of
O2?
Correct Answer: Binding of O2 on one subunit causes a conformational change in
adjacent subunit allowing O2 to bind.
• Question
Why does myoglobin not experience cooperative binding of O2?
Correct Answer: It's is monomeric.
• Question
What effect does an enzyme have on ΔG?
Correct Answer: None
• Question
What effect does an enzyme have on transition state?
Correct Answer: Reaches it quicker but doesn't stabilize it.
Answers Latest Updated 2026/2027 (Graded A+)
• Question
Which Amino Acids will be charged at pH 7?
Correct Answer: DEHRK: Aspartic Acid (Asp), Glutamic Acid (Glu), Histidine (His),
Arginine (Arg), Lysine (Lys)
• Question
Which amino acids will have a negative charge at pH 7?
Correct Answer: D&E: Aspartic Acid (Asp) and Glutamic acid (Glu)
• Question
Which amino acids will have a positive charge at pH 7?
Correct Answer: H, R, K: Histidine (His), Arginine (Arg), Lysine (Lys)
• Question
Which of the following sequences are most likely to be in the interior core of a globular
protein?
(A) ITFWLI (B) IKFGVA (C) DKYPGE (D) MPCDNA
Correct Answer: The interior is hydrophilic so sequence: ITFWLI is most likely to be in the
interior because there is no charged amino acids and had the lowest percentage of polar
amino acids.
• Question
How are beta sheets stabilized in proteins?
Correct Answer: Hydrogen bonding between peptide backbone groups.
• Question
Fibrous Proteins
Correct Answer: Not water soluble, not flexible in secondary structure, highly repetitive
primary structure, elongated structure; 2 α keratin polypeptides each which forms an α
helix twisting around each other to form a left handed coil.
• Question
Globular Proteins
Correct Answer: Water soluble, flexible in secondary structure, not necessarily highly
repetitive, size and ability to associate into a larger structure varies.
, • Question
Gel filtration elution
Correct Answer: Largest elutes first
• Question
Competitive inhibitor
Correct Answer: Vmax stays the same different km
• Question
Noncompetitive inhibitor
Correct Answer: Same X axis on reciprocal, same Km different vmax
• Question
Uncompetitive inhibitor
Correct Answer: Different Km and vmax
• Question
Catalytic efficiency
Correct Answer: Kcat/km higher is more efficient
• Question
What are the structural changes in hemoglobin that allows for cooperative binding of
O2?
Correct Answer: Binding of O2 on one subunit causes a conformational change in
adjacent subunit allowing O2 to bind.
• Question
Why does myoglobin not experience cooperative binding of O2?
Correct Answer: It's is monomeric.
• Question
What effect does an enzyme have on ΔG?
Correct Answer: None
• Question
What effect does an enzyme have on transition state?
Correct Answer: Reaches it quicker but doesn't stabilize it.
