Lab 7 Notebook
Title: DM07 Secondary Assays for Bacterial Characterization
Objective: To profile bacterial populations based on additional biochemical approaches to better
determine growth/metabolic characteristics.
Procedure: Test #1
1. Using a sterile loop pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine test area for color change within 20 seconds
Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown)
tryptophan into indole.
Procedure: Test #2
1. Using a sterile loop pick an isolated colony from BAP
2. Inoculate bacteria in media broth
3. Grow at 37 degrees Celsius overnight
4. Add- 5 drops of Kovac’s reagent to culture
Note:
Indole negative = Yellow color Indole
positive = Red/Pink orange color
Notes:
- Indole Test
- TSI Test
- API Test
lOMoAR cPSD| 67691079
Title: DM07 Secondary Assays for Bacterial Characterization
Objective: To profile bacterial populations based on additional biochemical approaches to better
determine growth/metabolic characteristics.
Procedure: Test #1
1. Using a sterile loop pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine test area for color change within 20 seconds
Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown)
tryptophan into indole.
Procedure: Test #2
1. Using a sterile loop pick an isolated colony from BAP
2. Inoculate bacteria in media broth
3. Grow at 37 degrees Celsius overnight
4. Add- 5 drops of Kovac’s reagent to culture
Note:
Indole negative = Yellow color Indole
positive = Red/Pink orange color
Notes:
- Indole Test
- TSI Test
- API Test
lOMoAR cPSD| 67691079
