Lab 6 Notebook
Title: DM06 Enzymatic Assays
Objective: To profile bacterial populations based on key enzymatic reactions and determine
growth/metabolic characteristics.
Procedure:
1. Using a sterile loop pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine test area for color change within 20 seconds
Notes:
- Oxidase Test
- Catalase Test
- Coagulase Test
- Lipase Test
Oxidase negative = no color change Catalase negative = no bubbles
Oxidase positive = purple color Catalase positive = bubbles
Coagulase Test: Qualitative test to determine the presence or absence of coagulase, an enzyme
that plays a role in the formation of blood clots.
Lipase Test: Qualitative test to determine the presence or absence of lipase, an enzyme that
hydrolyzes triglycerides (fatty acids/lipids)
Procedure:
1. Using a sterile loop carefully pick colony from plate
2. Smear colony directly onto the glass slide
lOMoAR cPSD| 67691079
Title: DM06 Enzymatic Assays
Objective: To profile bacterial populations based on key enzymatic reactions and determine
growth/metabolic characteristics.
Procedure:
1. Using a sterile loop pick an isolated colony from BAP
2. Smear directly onto the reaction area of the slide
3. Examine test area for color change within 20 seconds
Notes:
- Oxidase Test
- Catalase Test
- Coagulase Test
- Lipase Test
Oxidase negative = no color change Catalase negative = no bubbles
Oxidase positive = purple color Catalase positive = bubbles
Coagulase Test: Qualitative test to determine the presence or absence of coagulase, an enzyme
that plays a role in the formation of blood clots.
Lipase Test: Qualitative test to determine the presence or absence of lipase, an enzyme that
hydrolyzes triglycerides (fatty acids/lipids)
Procedure:
1. Using a sterile loop carefully pick colony from plate
2. Smear colony directly onto the glass slide
lOMoAR cPSD| 67691079
