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Lab 4 Notebook
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Title: CS04 Introduction to Growth Media
Objective: To understand the types and uses of growth media for the isolation and identification of
unknown bacterial samples
Procedure:
4-phase dilution streaking: clonal isolation
1. Using sterile loop spread culture in area 1
2. Using new sterile loop drag through the end of area 1 once 3. Using new
sterile loop drag through the end of area 2 once
4. Using new sterile loop drag through the end of area 3 once
*Use a back-and-forth pattern to dilute the culture in each zone
*Invert plate and incubate overnight at 37 degrees Celsius
Quadrant growth: rapid test for multiple isolates (non-selective agar) *Can
draw 4 quadrants on the bottom of the plate*
1. Using a sterile loop spread unknown culture A in area 1
2. Using a new sterile loop spread unknown culture B in area 2
3. Using a new sterile loop spread unknown culture C in area 3
4. Using a new sterile loop spread unknown culture D in area 4
*Use a back-and-forth pattern to dilute the culture in each zone. Do not mix quadrants. *Invert
plate and incubate overnight at 37 degrees Celsius
Lab 4 Notebook
Back to Home Page
Title: CS04 Introduction to Growth Media
Objective: To understand the types and uses of growth media for the isolation and identification of
unknown bacterial samples
Procedure:
4-phase dilution streaking: clonal isolation
1. Using sterile loop spread culture in area 1
2. Using new sterile loop drag through the end of area 1 once 3. Using new
sterile loop drag through the end of area 2 once
4. Using new sterile loop drag through the end of area 3 once
*Use a back-and-forth pattern to dilute the culture in each zone
*Invert plate and incubate overnight at 37 degrees Celsius
Quadrant growth: rapid test for multiple isolates (non-selective agar) *Can
draw 4 quadrants on the bottom of the plate*
1. Using a sterile loop spread unknown culture A in area 1
2. Using a new sterile loop spread unknown culture B in area 2
3. Using a new sterile loop spread unknown culture C in area 3
4. Using a new sterile loop spread unknown culture D in area 4
*Use a back-and-forth pattern to dilute the culture in each zone. Do not mix quadrants. *Invert
plate and incubate overnight at 37 degrees Celsius
