ASCP CG CERTIFICATION PRACTICE EXAMS
WITH CORRECT ANSWERS
What is the best agent to use against mycoplasma? - ANSWER-BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWER-comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - ANSWER-Colcemid - mitotic spindle
poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ANSWER-Logged
in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ANSWER-
Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ANSWER-Dissection
,Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ANSWER-Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm
is still seen around the mets, what is the most likely cause? - ANSWER-
innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ANSWER-Asceptic technique
When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - ANSWER-prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - ANSWER-Increading
the drying time by using warm slides
What is true of an inverted microscope? - ANSWER-the objectives are
beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - ANSWER-
DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
, When verifying if a specimen is appropriate for cytogenetic analysis, what is
the MOST important data to accompany the specimen? - ANSWER-Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - ANSWER-Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only
if: - ANSWER-Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator
are important to monitor to assess the source of the problem? - ANSWER-
Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed
under a phase contrast scope is: - ANSWER-Slide drying after the cells have
been dropped
What is the modal number (default number of chromosomes in a cell) for a
Down syndrome translocation carrier? - ANSWER-45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - ANSWER-Chorionic villi
What is a possible cause for a drying oven to not maintain a proper
temperature? - ANSWER-The door does not seal properly
WITH CORRECT ANSWERS
What is the best agent to use against mycoplasma? - ANSWER-BM cyclin - a
combination of two antibiotics (tiamulin and minocycline) that effecticely
targets mycoplasma growth
For successful harvest, each new batch of hypotonic solution should be
evaluated by: - ANSWER-comparing the new lot to a lot already in use
Colagenase, pronase, and trypsin are: - ANSWER-Proteolytic enzymes - break
down tissues into single-cell suspensions
When harvesting peripheral blood cultures, asceptic technique no longer
matters following incubation with: - ANSWER-Colcemid - mitotic spindle
poison that halts cells at metaphase
Samples that arrive at the cytogenetics lab should first be: - ANSWER-Logged
in to the system
Phytohemagglutinin (PHA) stimulates which type of cells into division? -
ANSWER-T-cell lymphocytes
To avoid culture failure, monitoring reagents is crucial. When logging a
culture failure, what is the MOST important to document? - ANSWER-
Expiration dates of reagents used
Which type of microscope is used when cleaning a CVS (chorionic villus)
sample? - ANSWER-Dissection
,Which cell type is most problematic if found in a CVS (chorionic villus)
sample? - ANSWER-Decidua (maternal tissue)
When looking at a slide under a phase contrast scope and a lot of cytoplasm
is still seen around the mets, what is the most likely cause? - ANSWER-
innappropriate slide drying time
What will help prevent microbial contamination and cross contamination of
cultures? - ANSWER-Asceptic technique
When there are not enough mets to complete the study of a patient/case,
what needs to be done next? - ANSWER-prepare additional slides from any
remaining sample
When looking at a dropped slide under a phase scope and mets are very
spread out and sparse, what could help fix the issue? - ANSWER-Increading
the drying time by using warm slides
What is true of an inverted microscope? - ANSWER-the objectives are
beneath the stage
High-resolution banding is indicated for a possible diagnosis of: - ANSWER-
DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial
syndrome
"velo" - soft palette,
"cardio" - congenital heart defects,
"facial" - unique abnormal facial features, short philtrum (upper lip indent),
tubular nose, wide spread eyes, etc
, When verifying if a specimen is appropriate for cytogenetic analysis, what is
the MOST important data to accompany the specimen? - ANSWER-Reason for
referral
Which type of specimen should be requested for a cytogenetic analysis for
turner syndrome? - ANSWER-Heparinized peripheral blood stored at room
temperature
(NOT FROZEN)
A blood sample can be used to study the cytogenetics of a malignancy only
if: - ANSWER-Dividing leukemic cells are present in circulation
All of the cultures in a CO2 incubator failed, what aspects of the incubator
are important to monitor to assess the source of the problem? - ANSWER-
Temperature,
CO2 level, and
Humidity level
The most important factor affecting the quality of chromosomes viewed
under a phase contrast scope is: - ANSWER-Slide drying after the cells have
been dropped
What is the modal number (default number of chromosomes in a cell) for a
Down syndrome translocation carrier? - ANSWER-45
What is the best tissue to use for both a good success rate and abnormality
detection in spontaneous abortion miscarriages? - ANSWER-Chorionic villi
What is a possible cause for a drying oven to not maintain a proper
temperature? - ANSWER-The door does not seal properly
