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MMG 301 Exam 1 Questions and Correct
Answers/ Latest Update / Already Graded
Coccus
Ans: (single circle)
Diplococcus
Ans:
Streptococcus
Ans:
Staphylococcus
Ans: (clustered circles)
Bacillus
Ans:
Spirochete
Ans:
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Filamentous
Ans:
Vibrio
Ans:
What kind of lenses do optical light microscopes have?
Ans: Glass lenses
What kind of lenses do electron microscopes have?
Ans: Magnet lenses
Magnification
Ans: How big we can make something
Resolution
Ans: Limits the size of what can be clearly seen
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Why do we use immersion oil?
Ans: Beneficial to light microscopy; increases numerical
aperature; not as much light gets through without the oil
Resolving Power Equation
Ans: (.5 x λ) / numerical aperature
List the steps on the Gram Stain
Ans: 1. Flood heat-fixed smear with crystal violet for 1 minute
2. Add iodine solution for 1 minute
3. Decolorize with alcohol for 20 seconds
4. Counterstain with safranin for 1-2 minutes
List the steps for the Acid-Fast Stain
Ans: 1. Small amount of organism suspended in a saline
solution is fixed on a slide
2. Slide is flooded with fuchsin and phenol for 3 minutes, gentle
rinsed with water
3. Slide is decolorized with 3% HCl in 70% alcohol until color
appears to be removed for 2 minutes, rinsed wth water
4. Slide is flooded with methylene blue counterstain for 30
seconds, rinsed with water, air-dried
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Phase Contrast Microscopy
Ans: Converts differences in refractive index wit hin unstained
cells into contrast (living cells)
Darkfield Microscopy
Ans: Utilizes cardoid condenser between light source and
specimen (good for looking at extremely thin cells)
Describe the hydrophobic head and the hydrophobic tail of a
phospholipid
Ans: Made of fatty acids linked to a glycerol phosphate by an
ester bond
Ampithatic Molecule
Ans: Molecule that contains both hydrophobic and hydrophilic
regions
Where are ester lipids found?
Ans: Bacteria Eukarya
All rights reserved © 2025/ 2026 |
MMG 301 Exam 1 Questions and Correct
Answers/ Latest Update / Already Graded
Coccus
Ans: (single circle)
Diplococcus
Ans:
Streptococcus
Ans:
Staphylococcus
Ans: (clustered circles)
Bacillus
Ans:
Spirochete
Ans:
All rights reserved © 2025/ 2026 |
, Page |2
Filamentous
Ans:
Vibrio
Ans:
What kind of lenses do optical light microscopes have?
Ans: Glass lenses
What kind of lenses do electron microscopes have?
Ans: Magnet lenses
Magnification
Ans: How big we can make something
Resolution
Ans: Limits the size of what can be clearly seen
All rights reserved © 2025/ 2026 |
, Page |3
Why do we use immersion oil?
Ans: Beneficial to light microscopy; increases numerical
aperature; not as much light gets through without the oil
Resolving Power Equation
Ans: (.5 x λ) / numerical aperature
List the steps on the Gram Stain
Ans: 1. Flood heat-fixed smear with crystal violet for 1 minute
2. Add iodine solution for 1 minute
3. Decolorize with alcohol for 20 seconds
4. Counterstain with safranin for 1-2 minutes
List the steps for the Acid-Fast Stain
Ans: 1. Small amount of organism suspended in a saline
solution is fixed on a slide
2. Slide is flooded with fuchsin and phenol for 3 minutes, gentle
rinsed with water
3. Slide is decolorized with 3% HCl in 70% alcohol until color
appears to be removed for 2 minutes, rinsed wth water
4. Slide is flooded with methylene blue counterstain for 30
seconds, rinsed with water, air-dried
All rights reserved © 2025/ 2026 |
, Page |4
Phase Contrast Microscopy
Ans: Converts differences in refractive index wit hin unstained
cells into contrast (living cells)
Darkfield Microscopy
Ans: Utilizes cardoid condenser between light source and
specimen (good for looking at extremely thin cells)
Describe the hydrophobic head and the hydrophobic tail of a
phospholipid
Ans: Made of fatty acids linked to a glycerol phosphate by an
ester bond
Ampithatic Molecule
Ans: Molecule that contains both hydrophobic and hydrophilic
regions
Where are ester lipids found?
Ans: Bacteria Eukarya
All rights reserved © 2025/ 2026 |
