ASU BIO 340 ACTUAL TEST QUESTIONS
WITH COMPLETE ANSWER KEY
●● types of nitrogenous bases
Answer: purines and pyrimidines
●● 1' (deoxyribose carbon atom numbering system)
Answer: the carbon attached to the nitrogenous base
●● 2' (deoxyribose carbon atom numbering system)
Answer: the deoxy carbon - only hydrogens attached
●● 3' (deoxyribose carbon atom numbering system)
Answer: carries the hydroxyl group
●● 4' (deoxyribose carbon atom numbering system)
Answer: binds to the O which binds to the 1' to make the ring
●● 5' (deoxyribose carbon atom numbering system)
Answer: carries the phosphate group
●● DNA strand polarity
, Answer: Each strand of DNA has distinct 5' and 3' ends creating polarity
within the backbone.
5' will have an -OH or phosphate group bonded to C-5' of the sugar,
while the 3' end has a free -OH on C-3 of the sugar.
●● Rules to apply
Answer: pyrimidine vs purine
A=T, C=G
3 hyrdogen bonds (C-G), 2 hydrogen bonds (A-T)
5' carbon vs 3' carbon
strands are anti-parallel
●● Chargaff's Rule
Answer: ratios are 1:1
A=T and C=G
must be double stranded
●● semiconservative replication
Answer: Method of DNA replication in which parental strands separate,
act as templates, and produce molecules of DNA with one parental DNA
strand and one new DNA strand
WITH COMPLETE ANSWER KEY
●● types of nitrogenous bases
Answer: purines and pyrimidines
●● 1' (deoxyribose carbon atom numbering system)
Answer: the carbon attached to the nitrogenous base
●● 2' (deoxyribose carbon atom numbering system)
Answer: the deoxy carbon - only hydrogens attached
●● 3' (deoxyribose carbon atom numbering system)
Answer: carries the hydroxyl group
●● 4' (deoxyribose carbon atom numbering system)
Answer: binds to the O which binds to the 1' to make the ring
●● 5' (deoxyribose carbon atom numbering system)
Answer: carries the phosphate group
●● DNA strand polarity
, Answer: Each strand of DNA has distinct 5' and 3' ends creating polarity
within the backbone.
5' will have an -OH or phosphate group bonded to C-5' of the sugar,
while the 3' end has a free -OH on C-3 of the sugar.
●● Rules to apply
Answer: pyrimidine vs purine
A=T, C=G
3 hyrdogen bonds (C-G), 2 hydrogen bonds (A-T)
5' carbon vs 3' carbon
strands are anti-parallel
●● Chargaff's Rule
Answer: ratios are 1:1
A=T and C=G
must be double stranded
●● semiconservative replication
Answer: Method of DNA replication in which parental strands separate,
act as templates, and produce molecules of DNA with one parental DNA
strand and one new DNA strand
