Cell Bio - Exam 1 -. Exam 1
Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s the basi c uni t of bi ol ogy? T he Cel l
What i s the study of cel l ul ar structure and organi zati on? Bi oI magi ng
What i s the study of chemi cal reacti ons of bi omol ecul es (enzyme-catal yzed
Bi ochemi stry
reacti ons, metabol i c pathways)?
What i s the study of i nteracti ons and regul ati on between cel l ul ar systems
Mol ecul ar Bi ol ogy
i nvol vi ng D NA, RNA and protei n synthesi s?
Who f i rst i nvented the l i ght mi croscope? Robert H ooke
Who coi ned the word cel l ? Robert H ooke
Who i mproved on the mi croscope? Antoni e van Leeuwenhoek
What i s one of the cel l bi ol ogi sts most i mportant tool s? T he Li ght Mi croscope
Page 1 of 24 1
, Cell Bio - Exam 1 -. Exam 1
Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s the l i mi t of resol uti on of l i ght mi croscopy? 0.2 um
What i s the advantage that the l i ght mi croscope has? Li ve-Cel l i magi ng
What i s the abi l i ty to di scri mi nate two cl osel y pl aced structures? Resol uti on
What i s a measure of the i ncrease i n the di ameter of a structure? Magni f i cati on
What i s a contrast that i nvol ves l i ght absorpti on? Ampl i tude Contrast
What i s a contrast that i nvol ves a change i n wave phase? Phase Contrast
What type of i nstrument uti l i zes a f ocused l aser beam to provi de an attracti ve or
Opti cal T weezers
repul si ve f orce?
What type of mi croscopy i s usef ul i n detecti ng speci f i c f l uorescent l abel ed
F l uorescence Mi croscopy
protei ns or mol ecul es?
Page 2 of 24 2
, Cell Bio - Exam 1 -. Exam 1
Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s a mol ecul e that wi l l emi t a characteri sti c wave l ength of l i ght when
F l uorochrome
exci ted by a speci f i c wave l ength?
What i s the strategy f or epi f l uorescence mi croscopy f or dead cel l s? I ndi rect i mmuno-f l uorescence
T wo, a pri mary (bi nds to the protei n) and a secondary (bi nds to the pri mary
H ow many anti bodi es are i nvol ved i n I ndi rect I mmuno-f l uorescence?
anti body)
I n i ndi rect i mmuno-f l uorescence where i s the f l uorescent mol ecul e attached? T o the secondary anti body
I ncreased cl ari ty of i mages Creati on of Opti cal Secti ons T hree D i mensi onal
What are the advantages of conf ocal mi croscopy?
I nf ormati on
What i s the strategy f or epi f l uorescence mi croscopy f or l i vi ng cel l s? Green F l uorescent Protei n i ntegrati on and dyes.
What are the two types of el ectron mi croscopy? Scanni ng E l ectron Mi croscopy T ransmi ssi on E l ectron Mi croscopy
Who devel oped the el ectron mi croscope? Knol l and Ruska
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Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s the basi c uni t of bi ol ogy? T he Cel l
What i s the study of cel l ul ar structure and organi zati on? Bi oI magi ng
What i s the study of chemi cal reacti ons of bi omol ecul es (enzyme-catal yzed
Bi ochemi stry
reacti ons, metabol i c pathways)?
What i s the study of i nteracti ons and regul ati on between cel l ul ar systems
Mol ecul ar Bi ol ogy
i nvol vi ng D NA, RNA and protei n synthesi s?
Who f i rst i nvented the l i ght mi croscope? Robert H ooke
Who coi ned the word cel l ? Robert H ooke
Who i mproved on the mi croscope? Antoni e van Leeuwenhoek
What i s one of the cel l bi ol ogi sts most i mportant tool s? T he Li ght Mi croscope
Page 1 of 24 1
, Cell Bio - Exam 1 -. Exam 1
Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s the l i mi t of resol uti on of l i ght mi croscopy? 0.2 um
What i s the advantage that the l i ght mi croscope has? Li ve-Cel l i magi ng
What i s the abi l i ty to di scri mi nate two cl osel y pl aced structures? Resol uti on
What i s a measure of the i ncrease i n the di ameter of a structure? Magni f i cati on
What i s a contrast that i nvol ves l i ght absorpti on? Ampl i tude Contrast
What i s a contrast that i nvol ves a change i n wave phase? Phase Contrast
What type of i nstrument uti l i zes a f ocused l aser beam to provi de an attracti ve or
Opti cal T weezers
repul si ve f orce?
What type of mi croscopy i s usef ul i n detecti ng speci f i c f l uorescent l abel ed
F l uorescence Mi croscopy
protei ns or mol ecul es?
Page 2 of 24 2
, Cell Bio - Exam 1 -. Exam 1
Cell Bio answers!!
Study this set o nline at: https://www.cram.co m/flashcards/cell-bio -exam-1-2998753
What i s a mol ecul e that wi l l emi t a characteri sti c wave l ength of l i ght when
F l uorochrome
exci ted by a speci f i c wave l ength?
What i s the strategy f or epi f l uorescence mi croscopy f or dead cel l s? I ndi rect i mmuno-f l uorescence
T wo, a pri mary (bi nds to the protei n) and a secondary (bi nds to the pri mary
H ow many anti bodi es are i nvol ved i n I ndi rect I mmuno-f l uorescence?
anti body)
I n i ndi rect i mmuno-f l uorescence where i s the f l uorescent mol ecul e attached? T o the secondary anti body
I ncreased cl ari ty of i mages Creati on of Opti cal Secti ons T hree D i mensi onal
What are the advantages of conf ocal mi croscopy?
I nf ormati on
What i s the strategy f or epi f l uorescence mi croscopy f or l i vi ng cel l s? Green F l uorescent Protei n i ntegrati on and dyes.
What are the two types of el ectron mi croscopy? Scanni ng E l ectron Mi croscopy T ransmi ssi on E l ectron Mi croscopy
Who devel oped the el ectron mi croscope? Knol l and Ruska
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