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AMT MLS EXAM 2026/2027
ACTUAL QUESTIONS WITH
VERIFIED ANSWERS.
Which immunoassay methodology is the emission of light by
molecules in an excited state with a limited amount of heat as
the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an
excited state with a limited amount of heat (luminescence) as
the result of a chemical reaction. Chemiluminescent assays are
ultrasensitive and are widely used in automated immunoassays
and DNA probe assay systems. Chemiluminescence has
excellent sensitivity and dynamic range. Chemiluminescent
labels can be attached to an antigen or an antibody. Most
chemiluminescent reagents and conjugates are stable and
relatively nontoxic.
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What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - a. Contains a known amount of
analyte being tested
Calibrators are used to establish a correlation between the
measurement response and the concentration or amount of the
substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission
using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission
using 2 - log10 %T. If all the light passes through a solution
without any absorption, absorbance is zero and the %T is
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100%. If all the light is absorbed, %T is zero, and absorption is
infinite.
What is a substance or constituent in which the laboratory
conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light
microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - d. study crystals in urine
and cells
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What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - b. Prevents
coagulation by inhibiting coagulation
CLSI recommends the salts of the chelating (calcium binding)
agent EDTA as the anticoagulant of choice for blood cell
counting and sizing.
How must a laboratory operate to handle TB sputum and TM
materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - a.
Biosafety level of 2+ or 3
BSL-3 builds upon the requirements of BSL-2. Organisms that
can cause serious or potentially lethal disease through
respiratory transmission are typically worked with in a BSL-3
laboratory. Example: Mycobacterium tuberculosis.
AMT MLS EXAM 2026/2027
ACTUAL QUESTIONS WITH
VERIFIED ANSWERS.
Which immunoassay methodology is the emission of light by
molecules in an excited state with a limited amount of heat as
the result of a chemical reaction?
Question
a.Chemiluminescence
b.Immunochromatography
c.Photometry
d.Spectrophotometry - a. Chemiluminescence
Chemiluminescence is the emission of light by molecules in an
excited state with a limited amount of heat (luminescence) as
the result of a chemical reaction. Chemiluminescent assays are
ultrasensitive and are widely used in automated immunoassays
and DNA probe assay systems. Chemiluminescence has
excellent sensitivity and dynamic range. Chemiluminescent
labels can be attached to an antigen or an antibody. Most
chemiluminescent reagents and conjugates are stable and
relatively nontoxic.
,2 | Page
What characteristics/functions do calibrators have?
Question
a.Contains a known amount of analyte being tested
b.Monitors the quality of reagents
c.Monitors the quality of the sample
d.Prevents equipment failure - a. Contains a known amount of
analyte being tested
Calibrators are used to establish a correlation between the
measurement response and the concentration or amount of the
substance that is being measured by the test procedure.
Absorbance can be calculated easily from percent transmission
using which calculation?
a.2 - log10 %T
b.2 + log10 %T
c.log10 %T - 2
d.log10 %T + 2 - a. 2 - log10 %T
Absorbance can be calculated easily from percent transmission
using 2 - log10 %T. If all the light passes through a solution
without any absorption, absorbance is zero and the %T is
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100%. If all the light is absorbed, %T is zero, and absorption is
infinite.
What is a substance or constituent in which the laboratory
conducts testing?
a.A solution
b.An additive
c.An analyte
d.An anticoagulant - c. An analyte
In the testing process, analytes or constituents are measured.
What does a common application of the polarized light
microscope in the clinical laboratory do?
Determine FANAs with fluorescence
b.Determine neutrophilic from lymphocytic leukemia
c.Examine histochemical reactions
d.Study crystals in urine and in cells - d. study crystals in urine
and cells
, 4 | Page
What is the chelation of calcium in the EDTA tube?
a.Maintains a constant temperature for components
b.Prevents coagulation by inhibiting coagulation
c.Prevents the separation of blood components
d.Stops the lysing of the red blood cells - b. Prevents
coagulation by inhibiting coagulation
CLSI recommends the salts of the chelating (calcium binding)
agent EDTA as the anticoagulant of choice for blood cell
counting and sizing.
How must a laboratory operate to handle TB sputum and TM
materials?
a.Biosafety level of 2+ or 3
b.Biosafety level 4
c.Location where air exchange is continuous
d.Location where fume hood exhausts are monitored daily - a.
Biosafety level of 2+ or 3
BSL-3 builds upon the requirements of BSL-2. Organisms that
can cause serious or potentially lethal disease through
respiratory transmission are typically worked with in a BSL-3
laboratory. Example: Mycobacterium tuberculosis.
