BACE Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
What is the primary purpose of a biosafety cabinet (BSC) To provide a sterile work environment and protect person-
in a biotechnology laboratory? nel from biohazards
What does the term 'sterile technique' specifically mean in Preventing any microorganisms from entering a sterile
a laboratory context? environment or culture
How often should the interior surfaces of a biological
Before and after each use session
safety cabinet be decontaminated with 70% ethanol?
A laboratory technician notices that a cell culture flask
contains turbid (cloudy) media that was previously clear. Microbial contamination has occured in the culture
What is most likely the explanation?
What is the correct order of operations when beginning Turn on blower than wait 5 mins for airflow to stabilize
work in a biological safety cabinet? them, then wipe with 70% ethanol to begin work
Which classification of biosafety cabinet is appropriate for
Class ll, Type B, or Class lll BSC
working with BSL-3 pathogens?
What is the significance of the 'dead zone' at the front Air turbulence at the cabinet, opening disrupts laminar
edge of a biosafety cabinet work surface? flow, making this area the least protected.
When performing an autoclave validation, what biological
Geobacillus stearothermophilus spores
indicator organism is typically used?
A technician must transfer a sterile solution from a large
Work inside the BSC, flame back neck if glass, pour without
bottle to a smaller container. What is the best aseptic
touching inside surfaces, recap immediately.
practice?
Which of the following is NOT a physical indicator of con-
Cells growing in a monolayer as expected.
tamination in a mammalian cell culture?
What is the purpose of using a 0.22 µm membrane filter To sterilize the solution by removing bacteria and larger
when preparing cell culture media supplements? microorganisms.
What is the correct procedure for calibrating a mi- Pipette a known volume of water and weigh it on an
cropipette before use? analytical balance to verify its accuracy.
A centrifuge rotor becomes unbalanced during a spin. Mechanical failure, rotor damage, or injury from rotor
What is the most immediate risk? failure.
, BACE Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
Which type of balance should be used when accurately
weighing 50 mg of a reagent for a critical butter prepara- An analytical balance with 0.1 mg readability.
tion?
What is the proper disposal procedure for used sharps
Dispose in a rigid, puncture-resistant sharps container.
(needles, scalpel blades) in a biotechnology laboratory?
When using a vortex mixer, what is the appropriate tech-
Press the tube bottom against the vortex pad at a slight
nique for mixing a small volume (100 µL) in a microcen-
angle at medium - low speed.
trifuge tube?
What does the abbreviation 'GLP' stand for in the context
Good Laboratory Practice.
of laboratory equipment use and data recording?
A spectrophotometer is used to measure the optical den-
To zero the absorbance against the growth media only
sity (OD600) of a bacterial culture.What is the purpose of
certain bacteria cell density is measured.
the 'blank' measurement?
Which type of pipette is most appropriate for accurately
20 mL graduated pipette
transferring exactly 15.7 mL of a solution?
Why must you never use a mercury thermometer in a Mercury vapor and liquid creates serious health and en-
laboratory incubator or water bath? vironmental hazards.
In a laboratory setting, what is the recommended fre-
Clean and calibrate after each session.
quency for cleaning and calibrating pH meters?
A biotechnician needs to confirm media has cooled
A calibrated digital thermocouple or infrared thermome-
enough after autoclaving before adding heat-sensitive
ter
supplements. Which tool is most appropriate?
What is the primary safety purpose of a chemical fume To protect laboratory workers from inhaling toxic vapors
hood in a laboratory? and fumes.
To maintain media pH by equilibrating with bicarbon-
What is the purpose of the CO2 in a cell culture incubator?
ate-buttered media.
What does the term 'passage number' refer to in cell
The number of times cells have been split.
culture?
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
What is the primary purpose of a biosafety cabinet (BSC) To provide a sterile work environment and protect person-
in a biotechnology laboratory? nel from biohazards
What does the term 'sterile technique' specifically mean in Preventing any microorganisms from entering a sterile
a laboratory context? environment or culture
How often should the interior surfaces of a biological
Before and after each use session
safety cabinet be decontaminated with 70% ethanol?
A laboratory technician notices that a cell culture flask
contains turbid (cloudy) media that was previously clear. Microbial contamination has occured in the culture
What is most likely the explanation?
What is the correct order of operations when beginning Turn on blower than wait 5 mins for airflow to stabilize
work in a biological safety cabinet? them, then wipe with 70% ethanol to begin work
Which classification of biosafety cabinet is appropriate for
Class ll, Type B, or Class lll BSC
working with BSL-3 pathogens?
What is the significance of the 'dead zone' at the front Air turbulence at the cabinet, opening disrupts laminar
edge of a biosafety cabinet work surface? flow, making this area the least protected.
When performing an autoclave validation, what biological
Geobacillus stearothermophilus spores
indicator organism is typically used?
A technician must transfer a sterile solution from a large
Work inside the BSC, flame back neck if glass, pour without
bottle to a smaller container. What is the best aseptic
touching inside surfaces, recap immediately.
practice?
Which of the following is NOT a physical indicator of con-
Cells growing in a monolayer as expected.
tamination in a mammalian cell culture?
What is the purpose of using a 0.22 µm membrane filter To sterilize the solution by removing bacteria and larger
when preparing cell culture media supplements? microorganisms.
What is the correct procedure for calibrating a mi- Pipette a known volume of water and weigh it on an
cropipette before use? analytical balance to verify its accuracy.
A centrifuge rotor becomes unbalanced during a spin. Mechanical failure, rotor damage, or injury from rotor
What is the most immediate risk? failure.
, BACE Comprehensive Resource To Help You Ace 2026-2027 Exams Includes Frequently Tested
Questions With ELABORATED 100% Correct COMPLETE SOLUTIONS
Guaranteed Pass First Attempt!! Current Update!!
Which type of balance should be used when accurately
weighing 50 mg of a reagent for a critical butter prepara- An analytical balance with 0.1 mg readability.
tion?
What is the proper disposal procedure for used sharps
Dispose in a rigid, puncture-resistant sharps container.
(needles, scalpel blades) in a biotechnology laboratory?
When using a vortex mixer, what is the appropriate tech-
Press the tube bottom against the vortex pad at a slight
nique for mixing a small volume (100 µL) in a microcen-
angle at medium - low speed.
trifuge tube?
What does the abbreviation 'GLP' stand for in the context
Good Laboratory Practice.
of laboratory equipment use and data recording?
A spectrophotometer is used to measure the optical den-
To zero the absorbance against the growth media only
sity (OD600) of a bacterial culture.What is the purpose of
certain bacteria cell density is measured.
the 'blank' measurement?
Which type of pipette is most appropriate for accurately
20 mL graduated pipette
transferring exactly 15.7 mL of a solution?
Why must you never use a mercury thermometer in a Mercury vapor and liquid creates serious health and en-
laboratory incubator or water bath? vironmental hazards.
In a laboratory setting, what is the recommended fre-
Clean and calibrate after each session.
quency for cleaning and calibrating pH meters?
A biotechnician needs to confirm media has cooled
A calibrated digital thermocouple or infrared thermome-
enough after autoclaving before adding heat-sensitive
ter
supplements. Which tool is most appropriate?
What is the primary safety purpose of a chemical fume To protect laboratory workers from inhaling toxic vapors
hood in a laboratory? and fumes.
To maintain media pH by equilibrating with bicarbon-
What is the purpose of the CO2 in a cell culture incubator?
ate-buttered media.
What does the term 'passage number' refer to in cell
The number of times cells have been split.
culture?
