2027 Exams Includes Frequently Tested Questions With
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1. What is an example of a product easily found in nature?
A. Synthetic polymer
B. Antibiotic
C. Recombinant insulin
D. Monoclonal antibody
Rationale: Many antibiotics were originally discovered as naturally occurring
compounds produced by microorganisms, unlike lab-engineered
biopharmaceuticals.
2. In a biotechnology lab, tap water is best used for:
A. Preparing buffer solutions
B. Washing glassware
C. Diluting DNA samples
D. Preparing cell culture media
Rationale: Tap water contains minerals and impurities unsuitable for precise
experiments, but is adequate for general glassware cleaning before final rinsing
with purified water.
3. The order of amino acids on a polypeptide chain is encoded by:
A. tRNA
B. mRNA
,C. rRNA
D. DNA polymerase
Rationale: mRNA carries the codon sequence directly translated into the amino
acid order during protein synthesis.
4. How do DNA molecules vary from one species to another?
A. They use entirely different nitrogenous bases
B. In the number of genes and non-coding regions
C. They have different double helix structures
D. Only some species have DNA
Rationale: While DNA's basic chemical structure is universal, genome size and
composition (gene number, non-coding sequences) vary significantly between
species.
5. What is agar?
A. A liquid nutrient broth
B. Solid media on which bacteria grow
C. A type of restriction enzyme
D. A staining dye for proteins
Rationale: Agar is a gel-like substance derived from seaweed, commonly used as a
solid growth surface for culturing microorganisms.
6. What part of an amino acid differs from one to another?
A. The amino group
B. The carboxyl group
C. The R group (side chain)
D. The central carbon atom
, Rationale: All amino acids share a common backbone structure; the variable R
group is what distinguishes one amino acid from another.
7. How can colorless proteins be monitored during electrophoresis?
A. Using ethidium bromide
B. Using PAGE loading dye
C. Using restriction enzymes
D. Using PCR primers
Rationale: Loading dye added to protein samples allows visual tracking of
migration progress during PAGE, since proteins themselves are often colorless.
8. Which of the following is an mRNA codon?
A. ATA
B. UAU
C. Tyrosine
D. All of the above
Rationale: mRNA codons are composed of RNA bases (using U instead of T); UAU is
a valid RNA codon, while ATA is a DNA sequence and Tyrosine is an amino acid, not
a codon.
9. What is the purpose of SDS during electrophoresis?
A. To amplify DNA fragments
B. To denature proteins and give them a negative charge
C. To stain the gel for visualization
D. To increase protein solubility only
Rationale: SDS coats proteins uniformly with negative charge and unfolds their
native structure, allowing separation based on size alone during PAGE.