GENETICS: A CONCEPTUAL APPROACH
ACTUAL TEST PAPER QUESTIONS AND
SOLUTIONS GRADED A+
◉ Neo+.
Answer: Allows cells to grow in presence of neomycin
◉ Shine Delgarno Sequence PRO ONLY.
Answer: Consensus sequence locked on the mRNA that is recognized
by the 16s rRNA in the small subunit of the ribosome, determines
where translation begins
◉ 65,536 bp repeat, how long is recognition.
Answer: Recognition sequence = 4^n=65,536 n=8
◉ 3 major mods to go from pre-mRNA to mature mRNA.
Answer: 1) poly A tail
2) 5 prime cap
3) spice out the introns
◉ Kozak Sequence
, EUKARYOTES.
Answer: ACCAUGG found at end of 5 prime The ribosome requires
this sequence, or a possible variation to initiate translation.
◉ Cleavage Consensus sequence.
Answer: AAUAAA at 3 prime end,
◉ 3 prime UTR.
Answer: three prime untranslated region (3'-UTR) is the section of
messenger RNA (mRNA) that immediately follows the translation
termination codon.
◉ TOPO I.
Answer: single strand breaks to create axis of rotation
◉ topo II: DNA Gyrase.
Answer: double stranded breaks to relieve tortional strain
◉ DNA POL I.
Answer: removes/replaces rna primers in pro replication
◉ DNA POL III.
ACTUAL TEST PAPER QUESTIONS AND
SOLUTIONS GRADED A+
◉ Neo+.
Answer: Allows cells to grow in presence of neomycin
◉ Shine Delgarno Sequence PRO ONLY.
Answer: Consensus sequence locked on the mRNA that is recognized
by the 16s rRNA in the small subunit of the ribosome, determines
where translation begins
◉ 65,536 bp repeat, how long is recognition.
Answer: Recognition sequence = 4^n=65,536 n=8
◉ 3 major mods to go from pre-mRNA to mature mRNA.
Answer: 1) poly A tail
2) 5 prime cap
3) spice out the introns
◉ Kozak Sequence
, EUKARYOTES.
Answer: ACCAUGG found at end of 5 prime The ribosome requires
this sequence, or a possible variation to initiate translation.
◉ Cleavage Consensus sequence.
Answer: AAUAAA at 3 prime end,
◉ 3 prime UTR.
Answer: three prime untranslated region (3'-UTR) is the section of
messenger RNA (mRNA) that immediately follows the translation
termination codon.
◉ TOPO I.
Answer: single strand breaks to create axis of rotation
◉ topo II: DNA Gyrase.
Answer: double stranded breaks to relieve tortional strain
◉ DNA POL I.
Answer: removes/replaces rna primers in pro replication
◉ DNA POL III.
