250+ Practice Questions with Verified Answers & Rationales
Question 1
1. What is the primary function of a clinical chemistry analyzer?
A) To count blood cells
B) To measure the concentration of chemical analytes in blood,
serum, plasma, or urine
C) To identify microorganisms
D) To analyze DNA sequences
Answer
Answer B: – To measure the concentration of chemical analytes in
blood, serum, plasma, or urine
Rationale
,Clinical chemistry analyzers measure analytes such as glucose,
electrolytes, enzymes, lipids, proteins, and hormones. They use
photometric, potentiometric, or other detection methods.
Hematology analyzers count cells; microbiology systems identify
microorganisms; molecular systems analyze DNA/RNA. ---
Question 2
2. Which detection method is most commonly used in chemistry
analyzers for measuring enzyme activity?
A) Turbidimetry
B) Photometry (absorbance)
C) Fluorometry
D) Potentiometry
Answer
Answer B: – Photometry (absorbance)
Rationale
Enzyme activity is typically measured by monitoring the change in
absorbance of a chromogenic substrate over time. Photometry
measures the amount of light absorbed by the reaction mixture,
which is proportional to the concentration of the product. ---
,Question 3
3. What is the principle of operation for an ion-selective electrode
(ISE) in an electrolyte analyzer?
A) Light absorption
B) Potential difference measurement across a selective membrane
C) Light scattering
D) Electrical conductivity
Answer
Answer B: – Potential difference measurement across a selective
membrane
Rationale
Ion-selective electrodes measure the potential difference between
a reference electrode and a sensing electrode with a membrane
that selectively binds a specific ion (Na⁺, K⁺, Cl⁻, Ca²⁺). The potential
is proportional to the logarithm of the ion activity. ---
Question 4
4. What is the function of a peristaltic pump in a clinical chemistry
analyzer?
, A) To generate high pressure
B) To move liquids through tubing without contact between the
fluid and pump components
C) To mix reagents
D) To control temperature
Answer
Answer B: – To move liquids through tubing without contact
between the fluid and pump components
Rationale
Peristaltic pumps use rollers that compress a flexible tube,
propelling fluid forward. This design prevents contamination by
isolating the fluid from pump components. They are widely used for
sample and reagent aspiration. ---
Question 5
5. Which of the following is a key component of a hematology
analyzer?
A) Monochromator
B) Flow cytometer or impedance counter
C) Centrifuge
D) Incubator