UIUC MCB 450: EXAM 2 STUDY UPDATED ACTUAL QUESTIONS AND CORRECT ANSWERS

UIUC MCB 450: EXAM 2 STUDY UPDATED ACTUAL
QUESTIONS AND CORRECT ANSWERS

Question:
1. A Genomic DNA Library
a)contains solely DNA sequences representative of RNA made at specific time of development of the
organism.
b)contains solely DNA sequences representative of the RNA made in response to environmental signal.
c)contains only amplified copies of a specific gene.
d) contains sequences representative of all the DNA of the organism.
e)contains solely the sequences of DNA representative of the genes that encode proteins.
Answer:
d is correct: "a set of host cells (or phage) that collectively contain all of the DNA sequences from the
genome of another organism."

Question:
2. The sequence of entire genomes has made it possible to examine the level of gene expression in a
particular cell tissue by using oligonucleotide probes to assess the level of mRNA from a particular gene.
This is done most effectively through the use of:
a) screening of genome libraries
b)DNA microarrays
c)gene disruption or knockout
d)RNA interference
e)Southern blotting
Answer:
e is correct :
Southern blotting

Question:
3. After treatment of a plasmid and a DNA fragment of interest with restriction enzymes, one wishes to
anneal the cohesive ends and covalently link the fragment into the plasmid. What enzyme will be needed?
a) DNA polymerase
b) reverse transcriptase
c) DNA ligase
d) DNA helicase
e) beta-galactosidase
Answer:
c is correct:
DNA ligaseProteins

Question:
4. SDS polyacrylamide electrophoresis could be used to do which of the following?
a) determine the molecular weight of an oligomeric (multisubunit)protein.
b) determine the molecular weights of subunits of an ligomeric protein.
c) purify a monomeric enzymes in its active form.
d)determine the folding state of a monomeric enzyme.

, Answer:
b is correct
determine the molecular weights of subunits of an ligomeric protein.

Question:
5. Proteins destined for excretion from the cell are ultimately synthesized on ribosomes located:
a) at the cell membrane
b) on the Golgi
c) in the cytoplasm
d) on the smooth endoplasmic reticulum
e) on the rough endoplasmic reticulum
Answer:
e is correct:
on the rough endoplasmic reticulum

Question:
6. The codon AUG speifies either initiation of protein synthesis or an internal methionine. What would be
the sequence of a tRNA that binds to this codon?
a) 5' - TAC - 3'
b) 5' - UAC - 3'
c) 5' - GUA - 3'
d) 5' - CAU - 3'
e) 5' - CAT - 3'
Answer:
d is correct:
5' - CAU - 3'

Question:
7. Which of the following is NOT true of tRNA?
a) tRNA contains an amino acid attachment site.
b) tRNA stands for transfer RNA.
c) tRNA contains a codon.
d)tRNA contains unusual bases.
e) tRNA is L-shaped.
Answer:
c is correct:
tRNA contains a codon

Question:
8. Reverse transcriptase, an RNA dependent DNA polymerase, differs from DNA polymerase delta by
which of the following?
a)Synthesizes DNA in the 5' - 3' direction.
b)Follows Watson-Crick base pair rules.
c)Synthesizes DNA in the 3' - 5' direction.
d)Contains 3' - 5' exonuclease activity
e)Can insert inosine into a growing DNA chain.
Answer:
d is correct: reverse transcriptase contains 3' - 5' exonuclease activity