UAMS-BLOOD BANK CASE DEVELOPMENT- EXAM 2 STUDY SET UPDATED ACTUAL QUESTIONS AND CORRECT ANSWERS

UAMS-BLOOD BANK CASE DEVELOPMENT- EXAM 2
STUDY SET UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS

Question:
1. polyspecific AHG
Answer:
Reagent containing both IgG and Complement

Question:
2. Monospecific AHG
Answer:
Reagent containing only IgG or complement

Question:
3. By chelating Ca+++ ions, which prevents the assembly of C1
Answer:
How does EDTA prevent the activation of complement making it ideal for performing a DAT?

Question:
4. Autoimmune hemolytic anemia HDN Transfusion Reactions Drug-Induced Cell Hemolysis
Answer:
Reasons to perform a DAT

Question:
5. Patient has no antibody in plasma Previous antibody is below detectable level antigen to patient
antibody not present on screening cells
Answer:
Reasons for Negative IAT

Question:
6. AHG was added to each test
Answer:
Addition of check cells insures that:

Question:
7. Detects antibodies in serum perform routine pre-transfusion testing includes incubation phase uses
monospecific AHG Uses Screening cells
Answer:
Features of IAT testing

Question:
8. Detects C3 or IgG directly on cells Positive in baby with ABO HDN

, Answer:
Features of DAT Testing

Question:
9. Uses polyspecific AHG Add check cells to all negatives wash red cells to remove free serum
immunoglobulins
Answer:
Features of both DAT and IAT testing

Question:
10. Too weak of a cell suspension Too strong of a cell suspension Inadequate Washing Failure to
centrifuge Contamination of AHG with serum Failure to ass LISS in IAT
Answer:
Reasons for False Negatives

Question:
11. Poly or autoagglutinable cells Saline contaminated with bacteria or other substances Dirty Glassware
Answer:
Reasons for False Positives

Question:
12. Auto Adsorption
Answer:
Test performed on a a patient with an warm autoantibody, can only be performed if the patient has not
been transfused in the last three months

Question:
13. negative IAT No previous history of identified antibody no tying discrepancies or other serologic
inconsistencies
Answer:
Criteria that must be met in order for only an initial spin crossmatch to be performed

Question:
14. Le(a) Le(b) and H
Answer:
Antigens present in secretions of patient that is type O with Le and Se genes

Question:
15. Warm autoantibody
Answer:
When all screening cells and the auto control are positive at the AHG phase, what would one suspect?

Question:
16. Multiple alloantibodies
Answer:
When both screening cells I and II are positive, there are multiple reaction strengths and they are positive
at multiple phases and the auto control is negative, what would one suspect?