BIOC - 384 SW 9 UPDATED ACTUAL QUESTIONS AND
CORRECT ANSWERS
Question:
Part 1.) What potential disadvantage does the alternate
pathway of fructose catabolism in the liver introduce as
compared to glucose catabolism?
Part 2.) Given the potential disadvantage caused by the
synthesis of fructose-1-phosphate in a liver cell, why is it
that an enzyme capable of catalyzing a reaction to
convert this form of fructose into glucose has not
evolved in a manner similar to the reaction that converts
galactose-1-phosphate into glucose-1-phosphate?
Answer:
1.) Fructose catabolism does not have as many regulatory steps as glucose
catabolism.
2.) C1 on fructose-1-phosphate cannot participate in an isomerization reaction
because it is phosphorylated.
Question:
Part 1.) Select only one of the four identical subunits of
the protein by clicking one of the green-outlined boxes
next to a subunit listed along the bottom of the player.
When the subunit is selected, it will be covered with
green x's.
Next, change the display of the selected subunit to
ribbon structure by clicking the dropdown menu in the
upper right corner and then clicking "Toggle Ribbon for
selection" on the Display line. Click "Toggle CPK for
selection" to remove the space-filling display. To remove
the green x's, click "Clear Selection" on the Misc line.
Determine the secondary structure(s) that is/are exhibited
in this polypeptide.
Part 2.) Which of the following best describes the
relationship among a subunit of the protein, metal
cofactors, and ligands?
Part 3.) If phosphofructokinase experienced a mutation
that interfered with substrate binding, then what other
enzyme is going to be most immediately impacted in
terms of accessing substrate?
Answer:
1.) both alpha helices and beta sheets
2.) A metal ion and ligand interact with the protein in locations proximal to one
another.
3.) aldolase
, Question:
Your turn:
The yeast Saccharomyces cerevisiae is well known for its
role in baking and beer and wine making, but it is also
frequently used as a model organism in scientific
laboratories. This organism has a relatively simple
genome that has been completely sequenced and is easy
to manipulate. S. cerevisiae is also inexpensive and easy
to grow in the laboratory.
In 2002, an experiment containing S. cerevisiae was flown
aboard NASA mission STS-112 with the goal of identifying
gene expression changes related to microgravity. The
yeast samples were grown in small cylindrical glass tubes
placed in a tightly closed, temperature-controlled
container. To initiate growth, media containing glucose
and other basic nutrients were auto-injected into a
chamber containing dormant yeast. After a pre-
determined growth period, a fixative was auto-injected
into the chamber to stop growth and protect the
contents until the cells could be isolated back on Earth.
Before the mission, while determining the appropriate
experimental growth period, what unexpected problem
did the researchers encounter when they first tested out
the equipment in the laboratory by growing yeast under
simulated microgravity conditions for a few hours?
Answer:
The researchers opened the closed container to discover that all the glass tubes
had exploded.
Question:
Part 1.) Label the graph below to show the effect of high
ATP, ADP, or fructose-2,6-bisphosphate on
phosphofructokinase-1 (PFK-1) activity.
Part 2.) Which of the following statements best describes
the effect of ADP on the activity of PFK-1?
Answer:
1.) C (Fructose-2,6-bisphosphate) > A (ADP) > B (ATP) (in image)
2.) High concentrations of ADP block the inhibitory effect of ATP by competing for
the same allosteric site on PFK-1.
Question:
CORRECT ANSWERS
Question:
Part 1.) What potential disadvantage does the alternate
pathway of fructose catabolism in the liver introduce as
compared to glucose catabolism?
Part 2.) Given the potential disadvantage caused by the
synthesis of fructose-1-phosphate in a liver cell, why is it
that an enzyme capable of catalyzing a reaction to
convert this form of fructose into glucose has not
evolved in a manner similar to the reaction that converts
galactose-1-phosphate into glucose-1-phosphate?
Answer:
1.) Fructose catabolism does not have as many regulatory steps as glucose
catabolism.
2.) C1 on fructose-1-phosphate cannot participate in an isomerization reaction
because it is phosphorylated.
Question:
Part 1.) Select only one of the four identical subunits of
the protein by clicking one of the green-outlined boxes
next to a subunit listed along the bottom of the player.
When the subunit is selected, it will be covered with
green x's.
Next, change the display of the selected subunit to
ribbon structure by clicking the dropdown menu in the
upper right corner and then clicking "Toggle Ribbon for
selection" on the Display line. Click "Toggle CPK for
selection" to remove the space-filling display. To remove
the green x's, click "Clear Selection" on the Misc line.
Determine the secondary structure(s) that is/are exhibited
in this polypeptide.
Part 2.) Which of the following best describes the
relationship among a subunit of the protein, metal
cofactors, and ligands?
Part 3.) If phosphofructokinase experienced a mutation
that interfered with substrate binding, then what other
enzyme is going to be most immediately impacted in
terms of accessing substrate?
Answer:
1.) both alpha helices and beta sheets
2.) A metal ion and ligand interact with the protein in locations proximal to one
another.
3.) aldolase
, Question:
Your turn:
The yeast Saccharomyces cerevisiae is well known for its
role in baking and beer and wine making, but it is also
frequently used as a model organism in scientific
laboratories. This organism has a relatively simple
genome that has been completely sequenced and is easy
to manipulate. S. cerevisiae is also inexpensive and easy
to grow in the laboratory.
In 2002, an experiment containing S. cerevisiae was flown
aboard NASA mission STS-112 with the goal of identifying
gene expression changes related to microgravity. The
yeast samples were grown in small cylindrical glass tubes
placed in a tightly closed, temperature-controlled
container. To initiate growth, media containing glucose
and other basic nutrients were auto-injected into a
chamber containing dormant yeast. After a pre-
determined growth period, a fixative was auto-injected
into the chamber to stop growth and protect the
contents until the cells could be isolated back on Earth.
Before the mission, while determining the appropriate
experimental growth period, what unexpected problem
did the researchers encounter when they first tested out
the equipment in the laboratory by growing yeast under
simulated microgravity conditions for a few hours?
Answer:
The researchers opened the closed container to discover that all the glass tubes
had exploded.
Question:
Part 1.) Label the graph below to show the effect of high
ATP, ADP, or fructose-2,6-bisphosphate on
phosphofructokinase-1 (PFK-1) activity.
Part 2.) Which of the following statements best describes
the effect of ADP on the activity of PFK-1?
Answer:
1.) C (Fructose-2,6-bisphosphate) > A (ADP) > B (ATP) (in image)
2.) High concentrations of ADP block the inhibitory effect of ATP by competing for
the same allosteric site on PFK-1.
Question:
