Portage BIOD171
Microbiology Exam 5
2026/27
Define the concept of universal precautions (short ANSWER) - ANSWER-Universal
precautions means any and all samples, whether known or unknown, are to be
treated as potentially hazardous (or pathogenic) materials
List at lease 3 observations a researcher would be sure to note while assessing
and unknown microbial sample (short ANSWER) - ANSWER-Need to note:
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
While observing an unknown sample of limited amounts, a researcher must
determine the following observations: 1) the presence of any motility and 2) its
Gram status using the same sample- the liquid sample cannot be divided. Which
would you determine first and why? (short ANSWER) - ANSWER-You must
determine motility before determining Gram status. Motility requires a wet
mount, while gram staining requires heat fixing the sample. If one were to begin
with the gram stain, the heat fixation process would kill the organism, making any
, observations regarding motility impossible. The correct approach would be to
place the liquid culture on a glass slide and determine its motility status. Next, the
same liquid culture can be heat fixed and gram stained.
A facultative anaerobe is a microorganism capable of growth under what
conditions? (short ANSWER) - ANSWER-A facultative anaerobe is capable of
growth under aerobic (with oxygen) and anaerobic (without oxygen) conditions
True or False: The Lancefield groups are used to subdivide antigenic groups of
alpha-hemolytic Streptococcus - ANSWER-False. The Lancefield groups are used to
subdivide beta-hemolytic strep
The distinctions for Lancefield subgroups lie in its: (select all that apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above - ANSWER-B. Carbohydrate composition of antigens
Rheumatic fever displays _____ hemolytic activity and occurs when _____ if left
untreated - ANSWER-Beta: strep. Pharyngitis
True or False: Under most circumstances, staphylococcus can be found in ~80% of
human population where it remains non-symptomatic - ANSWER-False.
Staphylococcus in only found (non-symptomatic) in ~30% of the human
population
Microbiology Exam 5
2026/27
Define the concept of universal precautions (short ANSWER) - ANSWER-Universal
precautions means any and all samples, whether known or unknown, are to be
treated as potentially hazardous (or pathogenic) materials
List at lease 3 observations a researcher would be sure to note while assessing
and unknown microbial sample (short ANSWER) - ANSWER-Need to note:
1. Size and shape
2. Any observable motility
3. Gram status (positive or negative)
While observing an unknown sample of limited amounts, a researcher must
determine the following observations: 1) the presence of any motility and 2) its
Gram status using the same sample- the liquid sample cannot be divided. Which
would you determine first and why? (short ANSWER) - ANSWER-You must
determine motility before determining Gram status. Motility requires a wet
mount, while gram staining requires heat fixing the sample. If one were to begin
with the gram stain, the heat fixation process would kill the organism, making any
, observations regarding motility impossible. The correct approach would be to
place the liquid culture on a glass slide and determine its motility status. Next, the
same liquid culture can be heat fixed and gram stained.
A facultative anaerobe is a microorganism capable of growth under what
conditions? (short ANSWER) - ANSWER-A facultative anaerobe is capable of
growth under aerobic (with oxygen) and anaerobic (without oxygen) conditions
True or False: The Lancefield groups are used to subdivide antigenic groups of
alpha-hemolytic Streptococcus - ANSWER-False. The Lancefield groups are used to
subdivide beta-hemolytic strep
The distinctions for Lancefield subgroups lie in its: (select all that apply)
A. Catalase activity
B. Carbohydrate composition of antigens
C. Hemolytic activity
D. All of the above - ANSWER-B. Carbohydrate composition of antigens
Rheumatic fever displays _____ hemolytic activity and occurs when _____ if left
untreated - ANSWER-Beta: strep. Pharyngitis
True or False: Under most circumstances, staphylococcus can be found in ~80% of
human population where it remains non-symptomatic - ANSWER-False.
Staphylococcus in only found (non-symptomatic) in ~30% of the human
population
