CHT
Exam Prep 2026 | Certified Histocompatibility Technologist Study
Guide | 300+ Practice Questions, Answers & Detailed Rationales
Study Guide Features
➢300+ practice questions
➢Detailed answers
➢Comprehensive rationales
➢HLA typing review
➢HLA genetics review
➢Crossmatching review
,CHT Exam Prep 2026 | Certified Histocompatibility
Technologist Study Guide | 300+ Practice
Questions, Answers & Detailed Rationales
1. What do ASHI Standards say about running both the optical and
fluorescent standards for flow cytometry?
• A. Run each time the instrument is turned on
• B. Run any time maintenance has occurred
• C. Run any time problems have occurred that could potentially affect
instrument function
• D. All of the above
Answer: D. All of the above
Rationale: ASHI Standards require that both optical and fluorescent
standards be run each time the flow cytometer is turned on, any time
maintenance has occurred, and any time problems have occurred that
could potentially affect instrument function. This ensures the instrument is
properly calibrated and functioning correctly before patient samples are
analyzed.
2. According to CLIA regulations (CFR 493.1278 Standard:
Histocompatibility (f)(2)), it is allowed to perform transplants
involving a kidney without having available results of final
crossmatch before the kidney is transplanted.
,• A. True
• B. False
Answer: B. False
Rationale: CLIA regulations require that final crossmatch results be available
before kidney transplantation. This is a critical safety measure to prevent
hyperacute rejection caused by preformed donor-specific antibodies.
3. What do ASHI standards say about the timing of the final
crossmatch prior to kidney transplantation?
• A. Perform crossmatching assay with sensitivity appropriate for the
clinical protocol established with the transplant center
• B. Have results of final crossmatches available before renal
transplantation
• C. If an emergency transplant is performed before the crossmatch
results are available, document the reason provided by the transplant
candidate's physician to the laboratory
• D. All of the above
Answer: D. All of the above
Rationale: ASHI Standards require that crossmatch assays be performed
with appropriate sensitivity, results be available before transplantation, and
any exceptions (emergency transplants) must be documented with the
physician's reason. This ensures patient safety while allowing for rare
emergency situations.
, 4. Based on ASHI Standards, which of the following is true about
crossmatching before transplantation of a solid organ?
• A. Reasons for failure to crossmatch before renal transplant must be
documented by the transplant candidate's physician
• B. Results of final crossmatches must be available before performing
heart transplantation
• C. The policy for crossmatching is written by the laboratory and does
not need input from the transplant program
• D. Crossmatching prior to transplantation with a deceased donor
kidney is not required
Answer: A. Reasons for failure to crossmatch before renal transplant
must be documented by the transplant candidate's physician
Rationale: ASHI Standards require documentation from the physician if a
crossmatch cannot be performed before renal transplantation. The
crossmatch policy should be developed collaboratively between the
laboratory and transplant program, and crossmatching is required for
kidney transplantation.
5. Which of the following are required by ASHI's standards for
transplant support?
Exam Prep 2026 | Certified Histocompatibility Technologist Study
Guide | 300+ Practice Questions, Answers & Detailed Rationales
Study Guide Features
➢300+ practice questions
➢Detailed answers
➢Comprehensive rationales
➢HLA typing review
➢HLA genetics review
➢Crossmatching review
,CHT Exam Prep 2026 | Certified Histocompatibility
Technologist Study Guide | 300+ Practice
Questions, Answers & Detailed Rationales
1. What do ASHI Standards say about running both the optical and
fluorescent standards for flow cytometry?
• A. Run each time the instrument is turned on
• B. Run any time maintenance has occurred
• C. Run any time problems have occurred that could potentially affect
instrument function
• D. All of the above
Answer: D. All of the above
Rationale: ASHI Standards require that both optical and fluorescent
standards be run each time the flow cytometer is turned on, any time
maintenance has occurred, and any time problems have occurred that
could potentially affect instrument function. This ensures the instrument is
properly calibrated and functioning correctly before patient samples are
analyzed.
2. According to CLIA regulations (CFR 493.1278 Standard:
Histocompatibility (f)(2)), it is allowed to perform transplants
involving a kidney without having available results of final
crossmatch before the kidney is transplanted.
,• A. True
• B. False
Answer: B. False
Rationale: CLIA regulations require that final crossmatch results be available
before kidney transplantation. This is a critical safety measure to prevent
hyperacute rejection caused by preformed donor-specific antibodies.
3. What do ASHI standards say about the timing of the final
crossmatch prior to kidney transplantation?
• A. Perform crossmatching assay with sensitivity appropriate for the
clinical protocol established with the transplant center
• B. Have results of final crossmatches available before renal
transplantation
• C. If an emergency transplant is performed before the crossmatch
results are available, document the reason provided by the transplant
candidate's physician to the laboratory
• D. All of the above
Answer: D. All of the above
Rationale: ASHI Standards require that crossmatch assays be performed
with appropriate sensitivity, results be available before transplantation, and
any exceptions (emergency transplants) must be documented with the
physician's reason. This ensures patient safety while allowing for rare
emergency situations.
, 4. Based on ASHI Standards, which of the following is true about
crossmatching before transplantation of a solid organ?
• A. Reasons for failure to crossmatch before renal transplant must be
documented by the transplant candidate's physician
• B. Results of final crossmatches must be available before performing
heart transplantation
• C. The policy for crossmatching is written by the laboratory and does
not need input from the transplant program
• D. Crossmatching prior to transplantation with a deceased donor
kidney is not required
Answer: A. Reasons for failure to crossmatch before renal transplant
must be documented by the transplant candidate's physician
Rationale: ASHI Standards require documentation from the physician if a
crossmatch cannot be performed before renal transplantation. The
crossmatch policy should be developed collaboratively between the
laboratory and transplant program, and crossmatching is required for
kidney transplantation.
5. Which of the following are required by ASHI's standards for
transplant support?
