PORTAGE LEARNING BIOD 171 MICROBIOLOGY
MODULE 3 COMPLETE STUDY GUIDE AND
PRACTICE QUESTIONS
◉ Micrometer.
Answer: (um) used to measure size of cells (10^6m) one-millionth
of a meter
◉ Nanometer.
Answer: (nm) measures cellular organelles and viruses (10^9m)
one-billionth of a meter
◉ Magnification.
Answer: increase in size of object
◉ Resolution.
Answer: Ability to distinguish between two points that are close
together(resolving power)
◉ Contrast.
Answer: Difference of light absorbance between two objects or the
object and its background
, ◉ True or False: Longer wavelengths increase resolving power.
Answer: False: Shorter wavelengths increase resolving power
◉ True or False: Hight Resolution= ability to see clearly and low
resolution: unable to see the object clearly.
Answer: True
◉ True or False: Most microorganisms are colorless.
Answer: True (Staining increases contrast)
◉ Light Microscopy.
Answer: Use visible light and blue wavelengths for improved
resolution, Magnification (1X-200X), Resolution: (10mm-200nm),
Whole Cells and Organelles
◉ Electron Microscopy.
Answer: uses electrons w/ specimens in a vacuum, Magnification:
(1,000x-1000,000x); Resolution:(1mm-0.1nm), Prokaryotic cells,
viruses, macromolecule and large atoms
◉ Probe Microscopy.
MODULE 3 COMPLETE STUDY GUIDE AND
PRACTICE QUESTIONS
◉ Micrometer.
Answer: (um) used to measure size of cells (10^6m) one-millionth
of a meter
◉ Nanometer.
Answer: (nm) measures cellular organelles and viruses (10^9m)
one-billionth of a meter
◉ Magnification.
Answer: increase in size of object
◉ Resolution.
Answer: Ability to distinguish between two points that are close
together(resolving power)
◉ Contrast.
Answer: Difference of light absorbance between two objects or the
object and its background
, ◉ True or False: Longer wavelengths increase resolving power.
Answer: False: Shorter wavelengths increase resolving power
◉ True or False: Hight Resolution= ability to see clearly and low
resolution: unable to see the object clearly.
Answer: True
◉ True or False: Most microorganisms are colorless.
Answer: True (Staining increases contrast)
◉ Light Microscopy.
Answer: Use visible light and blue wavelengths for improved
resolution, Magnification (1X-200X), Resolution: (10mm-200nm),
Whole Cells and Organelles
◉ Electron Microscopy.
Answer: uses electrons w/ specimens in a vacuum, Magnification:
(1,000x-1000,000x); Resolution:(1mm-0.1nm), Prokaryotic cells,
viruses, macromolecule and large atoms
◉ Probe Microscopy.