ASCP MLT ACTUAL EXAM | MEDICAL
LABORATORY TECHNICIAN QUESTIONS
AND CORRECT ANSWERS (VERIFIED
ANSWERS) ALREADY GRADED A+
Question 1
Which of the following are not considered normal flora of the gastrointestinal tract:
A. Lactobacillus
B. Clostridium
C. Peptostreptococcus
D. Shigella
Correct Answer
D;
Shigella and Salmonella are not found as normal flora in the gastrointestinal tract.
Question 2
Single erythrocyte inclusions which are large, round, smooth and purplish-blue
staining are most likely:
A. Howell-Jolly bodies
B. Heinz bodies
C. Basophilic stippling
D. Cabot rings
Correct Answer
A;
Howell-Jolly bodies are composed of DNA and appear as small round ball-like
inclusions inside the red cells. Usually only one Howell-Jolly body will be present in
each red cell.
Page 1 of 465
,Question 3
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell
and a swollen vesicle in a fungus culture are excellent clues indicative of:
A. Acremonium
B. Aspergillus
C. Paecilomyces
D. Penicillium
E. Scopulariopsis
Correct Answer
B;
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell
and a swollen vesicle in a fungus culture are excellent clues indicative of Aspergillus.
Aspergillus has the ability to infect primarily immunocompromised hosts, and
causes pneumonia and/or disseminates to other organs. Clinical specimens should
be inoculated onto primary isolation media, such as Sabouraud's dextrose agar.
Aspergillus spp. are fast growing and can be white, yellow, yellow-brown, brown to
black or green in color.
Question 4
Contact dermatitis is mediated by:
A. B lymphocytes
B. Macrophages
C. Polymorphonuclear cells
D. T lymphocytes
E. Chemokines
Correct Answer
D;
When an antigen comes in contact with the skin, the antigen is processed by cells in
the epidermis and come in contact with T lymphocytes. T lymphocytes recognize
the antigen as foreign and circulate through the bloodstream back to the epidermis
and produce an inflammatory response to eliminate the antigen, but this immune
response can produce a characteristic rash in the skin called contact dermititis.
Page 2 of 465
,Question 5
Which bacterial species is a common agent of neonatal bacteremia?
A. Streptococcus pyogenes
B. Listeria monocytogenes
C. Staphylococcus aureus
D. Enterococcus faecium
Correct Answer
B;
Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that
have a characteristic tumbling motility. They are found primarily in the environment
and in the human GI tract. Since L. monocytogenes multiplies intracellularly, control
of listeriosis requires cell-mediated immunity; thus, immunocompromised patients
(such as newborns) are at high risk.
Question 6
On an electronic cell counter, hemoglobin determination may be falsely elevated
caused by the presence of:
A. Lipemic or icteric plasma
B. Leukocytopenia or Leukocytosis
C. Rouleaux or agglutinated RBCs
D. Anemia or Polycythemia
Correct Answer
A;
Since hemoglobin is measured spectrophotometrically on hematology analzyers,
interference from lipemia or icteric specimens can lead to decreased light detected
and measured through the sample and therefore inaccurate hemoglobin results
occur.
Page 3 of 465
, Question 7
Which of the following thyroid function assays is affected least by pregnancy:
A. Total T3
B. Total T4
C. Free T4
Correct Answer
C;
Free T4 is affected the least by changes in plasma proteins during pregnancy.
Question 8
Which of the following is a presumptive test for the identification of Lancefield group
A Streptococcus:
A. Bacitracin susceptibility
B. Positive coagulase
C. Hippurate hydrolysis
D. Gram stain
Correct Answer
A;
Bacitracin susceptibility is a presumptive test for the identification of Lancefield
group A Streptococcus.
Page 4 of 465
LABORATORY TECHNICIAN QUESTIONS
AND CORRECT ANSWERS (VERIFIED
ANSWERS) ALREADY GRADED A+
Question 1
Which of the following are not considered normal flora of the gastrointestinal tract:
A. Lactobacillus
B. Clostridium
C. Peptostreptococcus
D. Shigella
Correct Answer
D;
Shigella and Salmonella are not found as normal flora in the gastrointestinal tract.
Question 2
Single erythrocyte inclusions which are large, round, smooth and purplish-blue
staining are most likely:
A. Howell-Jolly bodies
B. Heinz bodies
C. Basophilic stippling
D. Cabot rings
Correct Answer
A;
Howell-Jolly bodies are composed of DNA and appear as small round ball-like
inclusions inside the red cells. Usually only one Howell-Jolly body will be present in
each red cell.
Page 1 of 465
,Question 3
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell
and a swollen vesicle in a fungus culture are excellent clues indicative of:
A. Acremonium
B. Aspergillus
C. Paecilomyces
D. Penicillium
E. Scopulariopsis
Correct Answer
B;
The presence of hyaline, septate hyphae, and a young conidiophore with a foot cell
and a swollen vesicle in a fungus culture are excellent clues indicative of Aspergillus.
Aspergillus has the ability to infect primarily immunocompromised hosts, and
causes pneumonia and/or disseminates to other organs. Clinical specimens should
be inoculated onto primary isolation media, such as Sabouraud's dextrose agar.
Aspergillus spp. are fast growing and can be white, yellow, yellow-brown, brown to
black or green in color.
Question 4
Contact dermatitis is mediated by:
A. B lymphocytes
B. Macrophages
C. Polymorphonuclear cells
D. T lymphocytes
E. Chemokines
Correct Answer
D;
When an antigen comes in contact with the skin, the antigen is processed by cells in
the epidermis and come in contact with T lymphocytes. T lymphocytes recognize
the antigen as foreign and circulate through the bloodstream back to the epidermis
and produce an inflammatory response to eliminate the antigen, but this immune
response can produce a characteristic rash in the skin called contact dermititis.
Page 2 of 465
,Question 5
Which bacterial species is a common agent of neonatal bacteremia?
A. Streptococcus pyogenes
B. Listeria monocytogenes
C. Staphylococcus aureus
D. Enterococcus faecium
Correct Answer
B;
Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that
have a characteristic tumbling motility. They are found primarily in the environment
and in the human GI tract. Since L. monocytogenes multiplies intracellularly, control
of listeriosis requires cell-mediated immunity; thus, immunocompromised patients
(such as newborns) are at high risk.
Question 6
On an electronic cell counter, hemoglobin determination may be falsely elevated
caused by the presence of:
A. Lipemic or icteric plasma
B. Leukocytopenia or Leukocytosis
C. Rouleaux or agglutinated RBCs
D. Anemia or Polycythemia
Correct Answer
A;
Since hemoglobin is measured spectrophotometrically on hematology analzyers,
interference from lipemia or icteric specimens can lead to decreased light detected
and measured through the sample and therefore inaccurate hemoglobin results
occur.
Page 3 of 465
, Question 7
Which of the following thyroid function assays is affected least by pregnancy:
A. Total T3
B. Total T4
C. Free T4
Correct Answer
C;
Free T4 is affected the least by changes in plasma proteins during pregnancy.
Question 8
Which of the following is a presumptive test for the identification of Lancefield group
A Streptococcus:
A. Bacitracin susceptibility
B. Positive coagulase
C. Hippurate hydrolysis
D. Gram stain
Correct Answer
A;
Bacitracin susceptibility is a presumptive test for the identification of Lancefield
group A Streptococcus.
Page 4 of 465
