MICRO TEST 3 DR AL LSUE EXAM SCRIPT
TESTED QUESTIONS COMPLETE ANSWERS
GRADED A+
⩥ What is disinfection?
Answer: Destruction of vegetative pathogens on nonliving surfaces; may
not kill endospores
⩥ What is sanitization?
Answer: Reduces microbes to safe public health levels
⩥ What is antisepsis?
Answer: Destruction of microbes on living tissue
⩥ What is bacteriostatic?
Answer: Inhibits bacterial growth without killing bacteria
⩥ What is bactericidal?
Answer: Kills bacteria
⩥ What is a germicide?
Answer: Kills many microorganisms but not necessarily spores
,⩥ What is a sporicide?
Answer: Kills endospores
⩥ Can something be somewhat sterile?
Answer: No, something is either sterile or not sterile
⩥ Why does moist heat kill faster than dry heat?
Answer: Water transfers heat more efficiently and denatures proteins
faster
⩥ How does moist heat kill microbes?
Answer: Protein denaturation and membrane destruction
⩥ How does dry heat kill microbes?
Answer: Oxidation and dehydration
⩥ What temperature does an autoclave use?
Answer: 121°C
⩥ What pressure does an autoclave use?
Answer: 15 psi
, ⩥ What does an autoclave kill?
Answer: All microbes including endospores
⩥ Does boiling water sterilize?
Answer: No
⩥ Why doesn't boiling water sterilize?
Answer: Endospores can survive boiling
⩥ How does UV light kill microbes?
Answer: Creates thymine dimers in DNA
⩥ What is the major limitation of UV light?
Answer: Poor penetration
⩥ Why doesn't UV work through blankets or dust?
Answer: UV must directly contact the microbe
⩥ Where is UV commonly used?
Answer: Air, water, and exposed surface disinfection
TESTED QUESTIONS COMPLETE ANSWERS
GRADED A+
⩥ What is disinfection?
Answer: Destruction of vegetative pathogens on nonliving surfaces; may
not kill endospores
⩥ What is sanitization?
Answer: Reduces microbes to safe public health levels
⩥ What is antisepsis?
Answer: Destruction of microbes on living tissue
⩥ What is bacteriostatic?
Answer: Inhibits bacterial growth without killing bacteria
⩥ What is bactericidal?
Answer: Kills bacteria
⩥ What is a germicide?
Answer: Kills many microorganisms but not necessarily spores
,⩥ What is a sporicide?
Answer: Kills endospores
⩥ Can something be somewhat sterile?
Answer: No, something is either sterile or not sterile
⩥ Why does moist heat kill faster than dry heat?
Answer: Water transfers heat more efficiently and denatures proteins
faster
⩥ How does moist heat kill microbes?
Answer: Protein denaturation and membrane destruction
⩥ How does dry heat kill microbes?
Answer: Oxidation and dehydration
⩥ What temperature does an autoclave use?
Answer: 121°C
⩥ What pressure does an autoclave use?
Answer: 15 psi
, ⩥ What does an autoclave kill?
Answer: All microbes including endospores
⩥ Does boiling water sterilize?
Answer: No
⩥ Why doesn't boiling water sterilize?
Answer: Endospores can survive boiling
⩥ How does UV light kill microbes?
Answer: Creates thymine dimers in DNA
⩥ What is the major limitation of UV light?
Answer: Poor penetration
⩥ Why doesn't UV work through blankets or dust?
Answer: UV must directly contact the microbe
⩥ Where is UV commonly used?
Answer: Air, water, and exposed surface disinfection
