1|Page
MLS (Medical Laboratory Scientist) ASCP Exam ||Just Out!!!
Distingush between Pappenheimer bodies an Howell-Jolly
bodies: - ANSWER-Prussian blue stain would help differentiate
between the two. Prussian blue stains iron. Pappenheimer
bodies, which contain iron, will stain blue. Howell-Jolly bodies
will not stain with Prussian blue because they do no contain
iron. Both Howell-Jolly bodies and Pappenheimer bodies will
stain with Wright-Giemsa stain.
Symptomes of Acute leukemia: - ANSWER-Acute leukemia is
associated with bone pain, hepatosplenomegaly, and swollen
lymph nodes. In addition, infections (causing fevers) and
bleeding are common. A high white blood cell count with man
blasts in the peripheral blood and bone marrow, along with a
low platelet and rbc count are also highly associated with this
condition.
Differentiate between group D streptococcus, Group D
enterococcus, Group B strep and Group A: - ANSWER-Group D
streptococcus (bile esculin +, 6.5% NaCl -, PYR -)
Group D enterococcus (bile esculin +, 6.5% NaCl +, PYR +)
Group B strep (bile esculin -, 6.5% NaCl variable, PYR -)
Group A strep is beta hemolytic
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Sudan II will confirm the presence of: - ANSWER-Fat
How much does 1 unit of red blood cells raise the hemoglobin?
- ANSWER-One unit of RBCs increases the hemoglobin level by
approximately 1g/dL in an adult who is not actively bleeding
and has no other predisposing factors that would shorten the
survival of the transfused blood cells
Alpha thalassemia Major: - ANSWER-AKA as Hydrops Fetalis, is
exhibited when all four alpha chain gene loci are deleted or
non-functional. The alpha chain is crucial to the formation of
hemoglobin variants that can conduct normal oxygen transport
and gas exchange. The genetic code --/-- is incompatible with
life.
Which part of the immunoglobulin molecule is responsible for
activating complement? - ANSWER-Fc
Nephrotic syndrome: - ANSWER-Damage to the electrical
charges of the glomerular membrane, allowing the passage of
high molecular weight proteins and lipids occurs in nephrotic
syndrome. This results in markedly increased urine protein
,3|Page
levels, and the appearance of fatty casts and oval fat bodies that
are characteristic of nephrotic syndrome.
Average life span of platelets (in the body): - ANSWER-9.5 days
The anemia of chronic infection is characterized by: - ANSWER-
decreased serum iron levels; serum Fe levels low due to
sequestration in macrophages and hepatocytes
IgG Index: - ANSWER-The IgG index is used to determine if
increased IgG in CSF is due to increased production in the CNS
or contamination from a breach to the blood-brain barrier.
Which of the following is a characteristic of Factor XII
deficiency? - ANSWER-Negative bleeding history
Patients with a deficiency of factor XII tend to have thrombotic
complications. The do not have bleeding problems most likely
due to the lack of activation of fibrin lysis, also due to pathway
activation of IX by VIIa/TF complex as well as the activation of
Factor XI by thrombin
, 4|Page
Heinz bodies are: - ANSWER-Denatured hemoglobin inclusions
that are readily removed by the spleen.
Urobilinogen is formed in the: - ANSWER-Intestine
Causes of Vitamin B12 deficiency: - ANSWER-Pericious anemia
in relapse, Patients on chronic hemodialysis and hodgkin
disease
T lymphocytes that possess the CD8 surface marker mediate
which of the following T-cell functions? - ANSWER-Cytotoxic T
cells, which are capable of destroying targets such as tumor
cells and virus-infected cells, bear the CD8 surface marker, while
the other cell types listed ( delayed type hypersensitivity,
regulatory and helper) are positive for the CD4 surface marker
Intrinsic Pathway: - ANSWER-Prekallikrein, heavy molecular
weight kinogen, XII, XI, X, IX, VIII, V, II and I
Measured by APTT
Leukmoid Reaction: - ANSWER-
MLS (Medical Laboratory Scientist) ASCP Exam ||Just Out!!!
Distingush between Pappenheimer bodies an Howell-Jolly
bodies: - ANSWER-Prussian blue stain would help differentiate
between the two. Prussian blue stains iron. Pappenheimer
bodies, which contain iron, will stain blue. Howell-Jolly bodies
will not stain with Prussian blue because they do no contain
iron. Both Howell-Jolly bodies and Pappenheimer bodies will
stain with Wright-Giemsa stain.
Symptomes of Acute leukemia: - ANSWER-Acute leukemia is
associated with bone pain, hepatosplenomegaly, and swollen
lymph nodes. In addition, infections (causing fevers) and
bleeding are common. A high white blood cell count with man
blasts in the peripheral blood and bone marrow, along with a
low platelet and rbc count are also highly associated with this
condition.
Differentiate between group D streptococcus, Group D
enterococcus, Group B strep and Group A: - ANSWER-Group D
streptococcus (bile esculin +, 6.5% NaCl -, PYR -)
Group D enterococcus (bile esculin +, 6.5% NaCl +, PYR +)
Group B strep (bile esculin -, 6.5% NaCl variable, PYR -)
Group A strep is beta hemolytic
,2|Page
Sudan II will confirm the presence of: - ANSWER-Fat
How much does 1 unit of red blood cells raise the hemoglobin?
- ANSWER-One unit of RBCs increases the hemoglobin level by
approximately 1g/dL in an adult who is not actively bleeding
and has no other predisposing factors that would shorten the
survival of the transfused blood cells
Alpha thalassemia Major: - ANSWER-AKA as Hydrops Fetalis, is
exhibited when all four alpha chain gene loci are deleted or
non-functional. The alpha chain is crucial to the formation of
hemoglobin variants that can conduct normal oxygen transport
and gas exchange. The genetic code --/-- is incompatible with
life.
Which part of the immunoglobulin molecule is responsible for
activating complement? - ANSWER-Fc
Nephrotic syndrome: - ANSWER-Damage to the electrical
charges of the glomerular membrane, allowing the passage of
high molecular weight proteins and lipids occurs in nephrotic
syndrome. This results in markedly increased urine protein
,3|Page
levels, and the appearance of fatty casts and oval fat bodies that
are characteristic of nephrotic syndrome.
Average life span of platelets (in the body): - ANSWER-9.5 days
The anemia of chronic infection is characterized by: - ANSWER-
decreased serum iron levels; serum Fe levels low due to
sequestration in macrophages and hepatocytes
IgG Index: - ANSWER-The IgG index is used to determine if
increased IgG in CSF is due to increased production in the CNS
or contamination from a breach to the blood-brain barrier.
Which of the following is a characteristic of Factor XII
deficiency? - ANSWER-Negative bleeding history
Patients with a deficiency of factor XII tend to have thrombotic
complications. The do not have bleeding problems most likely
due to the lack of activation of fibrin lysis, also due to pathway
activation of IX by VIIa/TF complex as well as the activation of
Factor XI by thrombin
, 4|Page
Heinz bodies are: - ANSWER-Denatured hemoglobin inclusions
that are readily removed by the spleen.
Urobilinogen is formed in the: - ANSWER-Intestine
Causes of Vitamin B12 deficiency: - ANSWER-Pericious anemia
in relapse, Patients on chronic hemodialysis and hodgkin
disease
T lymphocytes that possess the CD8 surface marker mediate
which of the following T-cell functions? - ANSWER-Cytotoxic T
cells, which are capable of destroying targets such as tumor
cells and virus-infected cells, bear the CD8 surface marker, while
the other cell types listed ( delayed type hypersensitivity,
regulatory and helper) are positive for the CD4 surface marker
Intrinsic Pathway: - ANSWER-Prekallikrein, heavy molecular
weight kinogen, XII, XI, X, IX, VIII, V, II and I
Measured by APTT
Leukmoid Reaction: - ANSWER-
