AMT MEDICAL LABORATORY SCIENTIST
MT AMT EXAMINATION (LATEST UPDATE
2026/2027)— ACTUAL EXAM QUESTIONS WITH
VERIFIED CORRECT ANSWERS | GRADED A+
A Weil-Felix Reaction assay was once used commonly to test for febrile
agglutinins that indicated evidence of infection with several Rickettsial
diseases. The antigens used in this
assay are derived from
Select one:
a. Proteus vulgaris.
b. Brucella spp.
c. Escherichia coli.
d. Salmonella typhi. - Correct AnswerProteus vulgaris.
One of the most commonly used tests for antinuclear antibodies is
Select one:
a. indirect immunofluorescence.
b. equine serum agglutination.
c. a skin test.
d. reverse polarization. - Correct Answerindirect immunofluorescence.
Page 1 of 88
, AMT MEDICAL LABORATORY SCIENTIST
Toxin A/B produced by Clostridium difficile is
Select one:
a. detected in stool for a positive test.
b. detected in serum only.
c. detected only during the colonoscopy procedure.
d. cannot be tested. - Correct Answerdetected in stool for a positive
test.
When performing the Directogen™ Meningitis Combo Test on
Cerebrospinal Fluid (CSF), the test card or slide should be rotated on a
mechanical rotator for 10 minutes at 100rpm ± 2rpm.
The procedure requires use of a moistened humidifying cover during
this rotation period. Which of the following is most likely to occur if you
forget to use the moistened humidifying
cover?
Select one:
a. False positive due to evaporation bring the latex bead closer together
b. False negative due to evaporation of the CSF
c. False positive due to loss of temperature control
d. Loss of sample and reagent due to liquids not being contained by the
cover - Correct AnswerFalse positive due to evaporation bring the latex
bead closer together
Page 2 of 88
, AMT MEDICAL LABORATORY SCIENTIST
The level of autoantibodies is reported
Select one:
a. in mg/dl.
b. as a ratio.
c. as a titre.
d. as a positive/negative reaction. - Correct Answeras a titre
HEp-2 cells play which of the following roles when used in Fluorescent
Antinuclear Antibody
(FANA) testing?
Select one:
a. The substrate on the slide, containing the antinuclear antigens to
which the antinuclear antibodies, if present, will bind.
b. The fluorochrome, which will illuminate if the antibody to which they
are conjugated,
binds.
c. The HEp-2 is one of the antinuclear antibodies being tested for.
d. These are a control and display only one type of positive flurorescent
pattern to be
sure the test is working correctly. - Correct AnswerThe substrate on the
slide, containing the antinuclear antigens to which the antinuclear
antibodies, if present, will bind.
Page 3 of 88
, AMT MEDICAL LABORATORY SCIENTIST
While the light can be emitted as ultraviolet, visible or infrared,
____________ is the most
commonly emitted.
Select one:
a. blue
b. ultraviolet
c. infrared
d. visible - Correct Answervisible
Which of the following statements is true regarding hemolytic disease
of the newborn (HDN)?
Select one:
a. A condition when a mother makes an antibody to red cell antigens
from the fetus
b. IgM antibodies from the fetus
c. When the mother makes an antigen to the antibody of the fetus
d. When a mother produces cold antibodies - Correct AnswerA
condition when a mother makes an antibody to red cell antigens from
the fetus
Which immunohematology test is used to detect antibody bound to red
cells in-vivo and uses
the AHG reagent?
Page 4 of 88
MT AMT EXAMINATION (LATEST UPDATE
2026/2027)— ACTUAL EXAM QUESTIONS WITH
VERIFIED CORRECT ANSWERS | GRADED A+
A Weil-Felix Reaction assay was once used commonly to test for febrile
agglutinins that indicated evidence of infection with several Rickettsial
diseases. The antigens used in this
assay are derived from
Select one:
a. Proteus vulgaris.
b. Brucella spp.
c. Escherichia coli.
d. Salmonella typhi. - Correct AnswerProteus vulgaris.
One of the most commonly used tests for antinuclear antibodies is
Select one:
a. indirect immunofluorescence.
b. equine serum agglutination.
c. a skin test.
d. reverse polarization. - Correct Answerindirect immunofluorescence.
Page 1 of 88
, AMT MEDICAL LABORATORY SCIENTIST
Toxin A/B produced by Clostridium difficile is
Select one:
a. detected in stool for a positive test.
b. detected in serum only.
c. detected only during the colonoscopy procedure.
d. cannot be tested. - Correct Answerdetected in stool for a positive
test.
When performing the Directogen™ Meningitis Combo Test on
Cerebrospinal Fluid (CSF), the test card or slide should be rotated on a
mechanical rotator for 10 minutes at 100rpm ± 2rpm.
The procedure requires use of a moistened humidifying cover during
this rotation period. Which of the following is most likely to occur if you
forget to use the moistened humidifying
cover?
Select one:
a. False positive due to evaporation bring the latex bead closer together
b. False negative due to evaporation of the CSF
c. False positive due to loss of temperature control
d. Loss of sample and reagent due to liquids not being contained by the
cover - Correct AnswerFalse positive due to evaporation bring the latex
bead closer together
Page 2 of 88
, AMT MEDICAL LABORATORY SCIENTIST
The level of autoantibodies is reported
Select one:
a. in mg/dl.
b. as a ratio.
c. as a titre.
d. as a positive/negative reaction. - Correct Answeras a titre
HEp-2 cells play which of the following roles when used in Fluorescent
Antinuclear Antibody
(FANA) testing?
Select one:
a. The substrate on the slide, containing the antinuclear antigens to
which the antinuclear antibodies, if present, will bind.
b. The fluorochrome, which will illuminate if the antibody to which they
are conjugated,
binds.
c. The HEp-2 is one of the antinuclear antibodies being tested for.
d. These are a control and display only one type of positive flurorescent
pattern to be
sure the test is working correctly. - Correct AnswerThe substrate on the
slide, containing the antinuclear antigens to which the antinuclear
antibodies, if present, will bind.
Page 3 of 88
, AMT MEDICAL LABORATORY SCIENTIST
While the light can be emitted as ultraviolet, visible or infrared,
____________ is the most
commonly emitted.
Select one:
a. blue
b. ultraviolet
c. infrared
d. visible - Correct Answervisible
Which of the following statements is true regarding hemolytic disease
of the newborn (HDN)?
Select one:
a. A condition when a mother makes an antibody to red cell antigens
from the fetus
b. IgM antibodies from the fetus
c. When the mother makes an antigen to the antibody of the fetus
d. When a mother produces cold antibodies - Correct AnswerA
condition when a mother makes an antibody to red cell antigens from
the fetus
Which immunohematology test is used to detect antibody bound to red
cells in-vivo and uses
the AHG reagent?
Page 4 of 88
