BMSC 210 -Module 6 And 7-Practice
Questions with Solved Solutions 2026
Updated.
what is the important advantage of pfu pol of taq - Answer has proofreading activity
whats the difference between PRC and RT PCR - Answer RTPCR uses an RNA template whereas
PCR uses a DNA template
which objective would be best to use a southern rather than nothern blot - Answer determine
if a gene is present in a genome
a PCR rxn coies individual gene in vitro with what as primers - Answer pair of DNA
which time periods during PCR thermocycling is hottest - Answer denaturing DNA
Principle behind NA probe must contain - Answer a key complementary part of the target
gene sequence of interest
aragose gel are general used to - Answer ascertain the purity and size of DNA fragements
FISH - Answer fluorescent probe are used to target specific DNA or RNA in the cell
Ethidium bromide - Answer bind DNA in gels to make the molecules fluorescent and therefore
visualized
reverse transcriptase - Answer rna to cDNA
if foreign gene is clones into an expression host its important that host is - Answer not
produced the protein being studied
Expression vectors are designed to ensure that ________ can be efficiently ________. - Answer
mRNA
transcribed
one of the more formidable obstacles of mammalian gene is presence of - Answer introns
, genes encoding green fluorescent protein and b-galactosidase are typically used in cloning as -
Answer reporter genes
what molecular mechanism does site directed mutagenesis exploit to introduce mutation at
specific site - Answer flanking complementary bound nucleotides premit noncomplementary
base pairing
If a protein that could be toxic to the expression host needs to be expressed in large quantities,
then it is best to select an expression vector that - Answer is inducible
which process results in production of a hybrid polypeptide - Answer protein fusion
what makes euk transcription easier to isolate than bacterial transcription - Answer mRNA is
polyadenylated in euk
CRISPR genome editing was discovered in bacteria as - Answer seek and destroy foreign NA
is it important to choose codon preferred by the expression host itself - Answer true
ORF encode for - Answer polypeptides
transcriptome analysis useful for - Answer analyzing RNA thus it reveals which genes are
expressed under different conditions
most abundant gene in prok genome are - Answer those involved in metabolism
The entire complement of RNA produced under a given set of conditions is called a(n) - Answer
transcriptome
Sanger method relies on - Answer the incorporation of dideoxynucleotides that terminate
chain extension during DNA syn
one megabase pair of DNA encodes about - Answer 1000 orf
orf with a specific function is an example of - Answer annotation
Questions with Solved Solutions 2026
Updated.
what is the important advantage of pfu pol of taq - Answer has proofreading activity
whats the difference between PRC and RT PCR - Answer RTPCR uses an RNA template whereas
PCR uses a DNA template
which objective would be best to use a southern rather than nothern blot - Answer determine
if a gene is present in a genome
a PCR rxn coies individual gene in vitro with what as primers - Answer pair of DNA
which time periods during PCR thermocycling is hottest - Answer denaturing DNA
Principle behind NA probe must contain - Answer a key complementary part of the target
gene sequence of interest
aragose gel are general used to - Answer ascertain the purity and size of DNA fragements
FISH - Answer fluorescent probe are used to target specific DNA or RNA in the cell
Ethidium bromide - Answer bind DNA in gels to make the molecules fluorescent and therefore
visualized
reverse transcriptase - Answer rna to cDNA
if foreign gene is clones into an expression host its important that host is - Answer not
produced the protein being studied
Expression vectors are designed to ensure that ________ can be efficiently ________. - Answer
mRNA
transcribed
one of the more formidable obstacles of mammalian gene is presence of - Answer introns
, genes encoding green fluorescent protein and b-galactosidase are typically used in cloning as -
Answer reporter genes
what molecular mechanism does site directed mutagenesis exploit to introduce mutation at
specific site - Answer flanking complementary bound nucleotides premit noncomplementary
base pairing
If a protein that could be toxic to the expression host needs to be expressed in large quantities,
then it is best to select an expression vector that - Answer is inducible
which process results in production of a hybrid polypeptide - Answer protein fusion
what makes euk transcription easier to isolate than bacterial transcription - Answer mRNA is
polyadenylated in euk
CRISPR genome editing was discovered in bacteria as - Answer seek and destroy foreign NA
is it important to choose codon preferred by the expression host itself - Answer true
ORF encode for - Answer polypeptides
transcriptome analysis useful for - Answer analyzing RNA thus it reveals which genes are
expressed under different conditions
most abundant gene in prok genome are - Answer those involved in metabolism
The entire complement of RNA produced under a given set of conditions is called a(n) - Answer
transcriptome
Sanger method relies on - Answer the incorporation of dideoxynucleotides that terminate
chain extension during DNA syn
one megabase pair of DNA encodes about - Answer 1000 orf
orf with a specific function is an example of - Answer annotation
