BMSC 210: Module 1-5-Test Questions
and All Accurate Answers.
microorganism - Answer unicellular, multicellular, acellular
microscopic organisms
acellular - Answer lacking a cell
virus
Cellular - Answer 3 domains; bacteria, archea and eukarya
Spontaneous generation - Answer that living things arised from non-living matter
who disproved the spontaneuous generation theory - Answer louis pasteur
Swan Neck Experiment - Answer step 1: flask with swan neck- air could come in, dirt could not,
nothing grew in broth
step 2: flask without neck, introduced dust, things grew
This disproved spontaneous generation
Germ theory of disease - Answer microorganisms known as pathogens cause infectiours
diseases
miasma theory - Answer bad air causes disease
Robert Koch - Answer Developed the culture plate method to identify pathogens
Koch's postulate - Answer 1. pathogen should be found in the body of the sick & not in the
healthy 2. pathogen must be isolated and grown in a pure culture 3. when the cultured
pathogen are placed in a new host they should cause the same disease 4.the injected pathogen
should be isolated from the second host & it should be identical to the original.
coccus - Answer ball
rod - Answer rod shaped
, spirilium - Answer spiral rod shaped
spirochete - Answer long spirally cylinger
stalk hypha - Answer budding and appendaged bacteria
filamentous - Answer long thread like
Light microscopy - Answer living or dead sample
staining improves visualization
Electron Microscope - Answer dead sample
very powerful
greater resolution than light
How to gram stain - Answer 1. flood the heat-fixed smear with crystal violet for 1 min
2. add iodine solution for 1 min
3. decolorize with alcohol for 20 sec
4. counterstain with safarin for 1-2 min
gram + - Answer purple, thick cell wall, that is exposed to the external environment, no outer
membrane
gram - - Answer pink, has thinner cell wall that is protected from the external environment by
the outer membrane
Peptidoglycan - Answer unique to bac,
Gram + cell envelope - Answer thick cell wall of peptidoglycan, cytoplasmic membrane,
endospore formation, teichoic acid and lipoteichoic acid, teichoic gives a negative charge to the
cell surface
Gram - cell envelope - Answer outer membrane w LPS, porins, tonB dependent transport,
periplasm space, thin cell wall, cytoplasmic membrane, specialized secretion system
S-layer - Answer found in Gram +, - and archea
and All Accurate Answers.
microorganism - Answer unicellular, multicellular, acellular
microscopic organisms
acellular - Answer lacking a cell
virus
Cellular - Answer 3 domains; bacteria, archea and eukarya
Spontaneous generation - Answer that living things arised from non-living matter
who disproved the spontaneuous generation theory - Answer louis pasteur
Swan Neck Experiment - Answer step 1: flask with swan neck- air could come in, dirt could not,
nothing grew in broth
step 2: flask without neck, introduced dust, things grew
This disproved spontaneous generation
Germ theory of disease - Answer microorganisms known as pathogens cause infectiours
diseases
miasma theory - Answer bad air causes disease
Robert Koch - Answer Developed the culture plate method to identify pathogens
Koch's postulate - Answer 1. pathogen should be found in the body of the sick & not in the
healthy 2. pathogen must be isolated and grown in a pure culture 3. when the cultured
pathogen are placed in a new host they should cause the same disease 4.the injected pathogen
should be isolated from the second host & it should be identical to the original.
coccus - Answer ball
rod - Answer rod shaped
, spirilium - Answer spiral rod shaped
spirochete - Answer long spirally cylinger
stalk hypha - Answer budding and appendaged bacteria
filamentous - Answer long thread like
Light microscopy - Answer living or dead sample
staining improves visualization
Electron Microscope - Answer dead sample
very powerful
greater resolution than light
How to gram stain - Answer 1. flood the heat-fixed smear with crystal violet for 1 min
2. add iodine solution for 1 min
3. decolorize with alcohol for 20 sec
4. counterstain with safarin for 1-2 min
gram + - Answer purple, thick cell wall, that is exposed to the external environment, no outer
membrane
gram - - Answer pink, has thinner cell wall that is protected from the external environment by
the outer membrane
Peptidoglycan - Answer unique to bac,
Gram + cell envelope - Answer thick cell wall of peptidoglycan, cytoplasmic membrane,
endospore formation, teichoic acid and lipoteichoic acid, teichoic gives a negative charge to the
cell surface
Gram - cell envelope - Answer outer membrane w LPS, porins, tonB dependent transport,
periplasm space, thin cell wall, cytoplasmic membrane, specialized secretion system
S-layer - Answer found in Gram +, - and archea
