ACS Biochemistry Exam 100 Questions
and Answers Verified Solutions Latest
Update 2026/2027
Question:
thioester bond (1)
Answer:
compounds with functional group C-S-CoA-C (eg, in acetyl-CoA)
Question:
zwitterion (3)
Answer:
dipolar ion with both positive and negative groups but overall neutral charge; can act as either an
acid or base
Question:
Isoelectric point (pI) (chapter 3)
Answer:
the characteristic pH at which the net electric charge is zero
Question:
SDS - Sodium Dodecyl Sulfate (chap 3)
Answer:
detergent used to unfold proteins and give them uniform negative charge
Question:
SDS Page (3)
, Answer:
chromatography used to separate proteins based on mass. light proteins travel fast than heavier ones
Question:
PCR - Polymerase chain reaction (3)
Answer:
copies DNA multiple times to increase sample size
Question:
Isoelectric focusing (chapt 3)
Answer:
procedure used to determine the isoelectric pt (pI) of a protein. Protein migrates through gel until
pH = pI (net charge = 0)
Question:
Two-Dimensional Electrophoresis (3)
Answer:
combines isoelectric focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
Question:
specific activity (3)
Answer:
number of enzyme units per mg of total protein (a measure of enzyme purity)
Question:
activity (3)
Answer:
and Answers Verified Solutions Latest
Update 2026/2027
Question:
thioester bond (1)
Answer:
compounds with functional group C-S-CoA-C (eg, in acetyl-CoA)
Question:
zwitterion (3)
Answer:
dipolar ion with both positive and negative groups but overall neutral charge; can act as either an
acid or base
Question:
Isoelectric point (pI) (chapter 3)
Answer:
the characteristic pH at which the net electric charge is zero
Question:
SDS - Sodium Dodecyl Sulfate (chap 3)
Answer:
detergent used to unfold proteins and give them uniform negative charge
Question:
SDS Page (3)
, Answer:
chromatography used to separate proteins based on mass. light proteins travel fast than heavier ones
Question:
PCR - Polymerase chain reaction (3)
Answer:
copies DNA multiple times to increase sample size
Question:
Isoelectric focusing (chapt 3)
Answer:
procedure used to determine the isoelectric pt (pI) of a protein. Protein migrates through gel until
pH = pI (net charge = 0)
Question:
Two-Dimensional Electrophoresis (3)
Answer:
combines isoelectric focusing and SDS electrophoresis; separates proteins by both molecular
weight and pI
Question:
specific activity (3)
Answer:
number of enzyme units per mg of total protein (a measure of enzyme purity)
Question:
activity (3)
Answer:
