ASCP SCYM Exam Comprehensive
Questions (Frequently Tested) and
Complete Solutions Graded A+
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Sample Processing - Answer: Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
,CD2 - Answer: T cells: NK cells
CD3 - Answer: T cells
CD4 - Answer: T helper cells, monocytes (dimly expressed)
CD5 - Answer: T cells; Some B Cells
CD8 - Answer: T cytotoxic cells, macrophages
CD10 - Answer: Immature T, B Cells, PMN's
CD13 - Answer: Monocytes, PMN's
CD14 - Answer: Monocytes, PMN's (dimly expressed)
CD16 - Answer: NK cells, PMN's, monocytes
CD19 - Answer: B Cells
,CD20 - Answer: B Cells
CD25 - Answer: Activated T Cells
CD33 - Answer: Monocytes
CD34 - Answer: Stem/Progenitor Cells
CD45 - Answer: Leukocytes
CD56 - Answer: NK cells, monocytes (dimly expressed)
Anti-Kappa - Answer: B Cells
Anti-Lambda - Answer: B Cells
Tdt - Answer: Immature Lymphocytes
Monoclonal Antibody Staining Direct Staining - Answer: Rapid
single step staining
, Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - Answer:
Secondary amplifies single
A few labeled secondaries can detect many primaries
Clinical Applications of Flow - Answer: -Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist
to ensure the accurate reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) -
Answer: 2 Tubes: 1- CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte
population.
Questions (Frequently Tested) and
Complete Solutions Graded A+
Professional Academic Assistance Services
Services Offered
• Proctored Exam Assistance
• Online Class Management (Full Course Support)
• Exam Preparation & Study Materials
• Assignments and Coursework Support
• Essay and Research Paper Writing
• Discussion Posts & Responses
• Editing and Proofreading
• Confidential Academic Consultation
Contact Information
Email:
WhatsApp link: https://wa.me/254704846336
Fast Response | Confidential | Reliable Academic Support
Helping Students Achieve Academic Excellence
Sample Processing - Answer: Optimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition
,CD2 - Answer: T cells: NK cells
CD3 - Answer: T cells
CD4 - Answer: T helper cells, monocytes (dimly expressed)
CD5 - Answer: T cells; Some B Cells
CD8 - Answer: T cytotoxic cells, macrophages
CD10 - Answer: Immature T, B Cells, PMN's
CD13 - Answer: Monocytes, PMN's
CD14 - Answer: Monocytes, PMN's (dimly expressed)
CD16 - Answer: NK cells, PMN's, monocytes
CD19 - Answer: B Cells
,CD20 - Answer: B Cells
CD25 - Answer: Activated T Cells
CD33 - Answer: Monocytes
CD34 - Answer: Stem/Progenitor Cells
CD45 - Answer: Leukocytes
CD56 - Answer: NK cells, monocytes (dimly expressed)
Anti-Kappa - Answer: B Cells
Anti-Lambda - Answer: B Cells
Tdt - Answer: Immature Lymphocytes
Monoclonal Antibody Staining Direct Staining - Answer: Rapid
single step staining
, Can use multiple antibodies from the same host
Monoclonal Antibody Staining Indirect Staining - Answer:
Secondary amplifies single
A few labeled secondaries can detect many primaries
Clinical Applications of Flow - Answer: -Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist
to ensure the accurate reporting of flow cytometric results.
Lymphocytes Subset Enumeration (Immunophenotyping) -
Answer: 2 Tubes: 1- CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte
population.
