SCYM (ASCP) Quiz Comprehensive
Questions (Frequently Tested) and
Complete Solutions Graded A+
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Most modern flow cytometers tightly position the sample for
optical analysis via hydrodynamic focusing. Here, a carrier fluid
called the sheath fluid is used to position the sample of cells into a
single file for optical interrogation. - Answer: Hydrodynamic
Focusing
,The central stream (sample stream) is focused and surrounded by
the secondary slower stream (sheath fluid). The shape and size of
the flow cell is crucial to hydrodynamic focusing, and traditionally
the cell is nozzle shaped. ... In a flow cytometer, the sheath fluid
pressure is constant while the sample fluid is adjusted - Answer:
Hydordynamic focusing and sheath fluids
The difference between the sample pressure and the sheath
pressure is the differential pressure. This controls the width of the
core stream and the total number of cells passing the laser
intercept. - Answer: Sample Pressure and the Sheath Pressure
Differential pressure based flow cytometers currently dominate
the market. These systems have two pressure regulators. The first
is at a constant pressure that sets how fast the fluids runs at. The
second is regulated by the investigator (like on this LSR-II control
panel). - Answer: differential pressure based flow cytometers
Low differential pressure allows the cells to move past the
interrogation point one at a time. .... One kind involves generating
pressure using a pump and regulator system ... Differential
pressure based fluidic system. ... peristaltic and/or syringe pumps
to deliver the sample into the instrument. - Answer: Generation
of differential pressure (syringe pump, pressure based)
,In syringe-pump-driven microfluidic systems, pressure
fluctuations are observed in an elastic microchannel. The syringe
pump is driven by an electrical stepper motor, from which
mechanical oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure fluctuations in the
channel flow. - Answer: Characterization of syringe-pump-driven
induced pressure
Filters are pieces of glass coated on both sides that allow light of a
certain collection, or band, of wavelengths to pass through while
absorbing or interfering with photons of other wavelengths.
These come in bandpass, longpass, and shortpass flavors -
Answer: Optical Filters
A filter that allows light between a set wavelength to pass through
and reflects light above and below the set wavelength. For
example, a bandpass filter with a wavelength of 550/40nm would
allow light between 530nm and 570nm to pass through, but
reflect light below 530nm and above 570nm. - Answer: Band Pass
Optical Filter
wavelength above 650nM - Answer: Longpass Filter
wavelength below 488nM - Answer: Shortpass Filter
, Dichroic mirrors can block light by phased reflection allowing
certain light to pass through and interfering with other
wavelengths. For example, a 500LP dichroic mirror would transmit
light above 500 nm and reflect the light below 500 nm in a
different direction. A 525SP dichroic mirror would transmit all
light below 525 nm and reflect all light above 525 nm in a
different direction. These dichroic mirrors are critical in the
directing and capturing of light by the detectors. - Answer:
dichroics mirrors
filter that reduces or modifies the intensity of all wavelengths, or
colors, of light equally, giving no changes in hue of color rendition
- Answer: neutral density filter
Polarization of scatter and fluorescence signals in flow
cytometry. ... depending on the light source(s), the optical layout,
and the types of mirrors and filters used. - Answer: polarization
filter
The light source can be a laser, an arc lamp or even an LED. Today,
the majority of instruments use a laser. Lasers illuminate the
stream with coherent, focused light of specific wavelength
(energy) and power. This illumination facilitates the generation of
Questions (Frequently Tested) and
Complete Solutions Graded A+
Professional Academic Assistance Services
Services Offered
• Proctored Exam Assistance
• Online Class Management (Full Course Support)
• Exam Preparation & Study Materials
• Assignments and Coursework Support
• Essay and Research Paper Writing
• Discussion Posts & Responses
• Editing and Proofreading
• Confidential Academic Consultation
Contact Information
Email:
WhatsApp link: https://wa.me/254704846336
Fast Response | Confidential | Reliable Academic Support
Helping Students Achieve Academic Excellence
Most modern flow cytometers tightly position the sample for
optical analysis via hydrodynamic focusing. Here, a carrier fluid
called the sheath fluid is used to position the sample of cells into a
single file for optical interrogation. - Answer: Hydrodynamic
Focusing
,The central stream (sample stream) is focused and surrounded by
the secondary slower stream (sheath fluid). The shape and size of
the flow cell is crucial to hydrodynamic focusing, and traditionally
the cell is nozzle shaped. ... In a flow cytometer, the sheath fluid
pressure is constant while the sample fluid is adjusted - Answer:
Hydordynamic focusing and sheath fluids
The difference between the sample pressure and the sheath
pressure is the differential pressure. This controls the width of the
core stream and the total number of cells passing the laser
intercept. - Answer: Sample Pressure and the Sheath Pressure
Differential pressure based flow cytometers currently dominate
the market. These systems have two pressure regulators. The first
is at a constant pressure that sets how fast the fluids runs at. The
second is regulated by the investigator (like on this LSR-II control
panel). - Answer: differential pressure based flow cytometers
Low differential pressure allows the cells to move past the
interrogation point one at a time. .... One kind involves generating
pressure using a pump and regulator system ... Differential
pressure based fluidic system. ... peristaltic and/or syringe pumps
to deliver the sample into the instrument. - Answer: Generation
of differential pressure (syringe pump, pressure based)
,In syringe-pump-driven microfluidic systems, pressure
fluctuations are observed in an elastic microchannel. The syringe
pump is driven by an electrical stepper motor, from which
mechanical oscillations are expected to generate flow-rate
fluctuations and in turn leads to the pressure fluctuations in the
channel flow. - Answer: Characterization of syringe-pump-driven
induced pressure
Filters are pieces of glass coated on both sides that allow light of a
certain collection, or band, of wavelengths to pass through while
absorbing or interfering with photons of other wavelengths.
These come in bandpass, longpass, and shortpass flavors -
Answer: Optical Filters
A filter that allows light between a set wavelength to pass through
and reflects light above and below the set wavelength. For
example, a bandpass filter with a wavelength of 550/40nm would
allow light between 530nm and 570nm to pass through, but
reflect light below 530nm and above 570nm. - Answer: Band Pass
Optical Filter
wavelength above 650nM - Answer: Longpass Filter
wavelength below 488nM - Answer: Shortpass Filter
, Dichroic mirrors can block light by phased reflection allowing
certain light to pass through and interfering with other
wavelengths. For example, a 500LP dichroic mirror would transmit
light above 500 nm and reflect the light below 500 nm in a
different direction. A 525SP dichroic mirror would transmit all
light below 525 nm and reflect all light above 525 nm in a
different direction. These dichroic mirrors are critical in the
directing and capturing of light by the detectors. - Answer:
dichroics mirrors
filter that reduces or modifies the intensity of all wavelengths, or
colors, of light equally, giving no changes in hue of color rendition
- Answer: neutral density filter
Polarization of scatter and fluorescence signals in flow
cytometry. ... depending on the light source(s), the optical layout,
and the types of mirrors and filters used. - Answer: polarization
filter
The light source can be a laser, an arc lamp or even an LED. Today,
the majority of instruments use a laser. Lasers illuminate the
stream with coherent, focused light of specific wavelength
(energy) and power. This illumination facilitates the generation of
