University of Houston UH BCHS 3201 LAB || Solved Correctly.
In PAGE, what is used to coat the proteins with a negative charge correct answers SDS
What is the difference between Coomassie Stain vs. Western Blot? correct answers Coomassie is
nonspecific, and Western Blot uses antibodies to identify specific proteins
What is the purpose of the Kaleidoscope marker/ Spectra Ladder in SDS PAGE? correct answers
It helps
determine molecular weight of the proteins
Why do you see a smear of bands when serum is run through PAGE with Coomassie
Stain? correct answers There are many proteins in the sera. The serum is a heterogeneous
mixture of proteins, and they are supposed to be separate bands; however, there are so many
proteins that, on the gel, they just appear to be a smear.
Which goes through the gel faster in PAGE? correct answers a. Protein A: 98 kDa
b. Protein B: 115 kDa
Which scientist received the Nobel prize for showing that X-rays are a form of
electromagnetic radiation and that crystals are periodic arrangements of atoms and so
should diffract x-rays? correct answers Max von Laue (He also developed equations to describe
the diffraction of x-rays by crystals but Braggs law is an improved version of those equations)
Who developed a simpler equation for describing the diffraction of x-rays by crystals and
received a Nobel Prize for it? correct answers Father and son pair William Henry Bragg and
William Lawrence Bragg
In the hanging drop crystallography method, the buffer in the reservoir is higher or lower
, in ionic strength compared to the buffer in the hanging drop? correct answers Higher
Crystal x-ray diffraction data is converted to what? correct answers Electron density map
For a small protein <25 kDa, what method other than protein crystallization can be used
to resolve the protein? correct answers NMR
What is the purpose of lysozyme? correct answers It cleaves the B-1,4 linkages between
Nacetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls to weaken them
(bacteriolytic, prevents bacterial invasion of developing chick embryos) and prevents bacterial
infection of the tissue (specifically nasal passages and conjunctiva around the eye)
In size exclusion chromatography, what is the order of elution if purifying the following
components? correct answers *biggest to smallest*
a. Hgb - 65,000 Da (2)
b. Myoglobin - 17,000 Da (3)
c. Mysoin - 180,000 Da (1)
. Describe Stoke's radius. correct answers The effective radius of a molecule moving through the
medium based on a perfect sphere, the effective radius of a molecule as it tumbles
through space/the column
True or False: To fully resolve the protein structure, you need to know the amino acid sequence
correct answers True
Students load a protein sample in a size exclusion chromatography Sephadex column.
What is the difference in the graph of absorbance vs. fraction number if the students
loaded the buffer right away after loading the protein sample vs. if the students ran the
In PAGE, what is used to coat the proteins with a negative charge correct answers SDS
What is the difference between Coomassie Stain vs. Western Blot? correct answers Coomassie is
nonspecific, and Western Blot uses antibodies to identify specific proteins
What is the purpose of the Kaleidoscope marker/ Spectra Ladder in SDS PAGE? correct answers
It helps
determine molecular weight of the proteins
Why do you see a smear of bands when serum is run through PAGE with Coomassie
Stain? correct answers There are many proteins in the sera. The serum is a heterogeneous
mixture of proteins, and they are supposed to be separate bands; however, there are so many
proteins that, on the gel, they just appear to be a smear.
Which goes through the gel faster in PAGE? correct answers a. Protein A: 98 kDa
b. Protein B: 115 kDa
Which scientist received the Nobel prize for showing that X-rays are a form of
electromagnetic radiation and that crystals are periodic arrangements of atoms and so
should diffract x-rays? correct answers Max von Laue (He also developed equations to describe
the diffraction of x-rays by crystals but Braggs law is an improved version of those equations)
Who developed a simpler equation for describing the diffraction of x-rays by crystals and
received a Nobel Prize for it? correct answers Father and son pair William Henry Bragg and
William Lawrence Bragg
In the hanging drop crystallography method, the buffer in the reservoir is higher or lower
, in ionic strength compared to the buffer in the hanging drop? correct answers Higher
Crystal x-ray diffraction data is converted to what? correct answers Electron density map
For a small protein <25 kDa, what method other than protein crystallization can be used
to resolve the protein? correct answers NMR
What is the purpose of lysozyme? correct answers It cleaves the B-1,4 linkages between
Nacetylmuramic acid and N-acetyl-D-glucosamine in bacterial cell walls to weaken them
(bacteriolytic, prevents bacterial invasion of developing chick embryos) and prevents bacterial
infection of the tissue (specifically nasal passages and conjunctiva around the eye)
In size exclusion chromatography, what is the order of elution if purifying the following
components? correct answers *biggest to smallest*
a. Hgb - 65,000 Da (2)
b. Myoglobin - 17,000 Da (3)
c. Mysoin - 180,000 Da (1)
. Describe Stoke's radius. correct answers The effective radius of a molecule moving through the
medium based on a perfect sphere, the effective radius of a molecule as it tumbles
through space/the column
True or False: To fully resolve the protein structure, you need to know the amino acid sequence
correct answers True
Students load a protein sample in a size exclusion chromatography Sephadex column.
What is the difference in the graph of absorbance vs. fraction number if the students
loaded the buffer right away after loading the protein sample vs. if the students ran the
