Biochemistry 3304 Final Exam || with Certified Answers.
What fundamental point about evolutionary processes is NOT correct? correct answers Variation
among individuals is directed.
What is the First Law of Thermodynamics? correct answers The total energy of any entire
system must be the same before and after an action or reaction.
What is the Second Law of Thermodynamics? correct answers S > 0 for spontaneous processes
What is the Equilibrium Gibbs Free Energy for a reaction at human physiological temperature
for a
reaction A B where, at equilibrium, [B] is 100 times that of [A]? correct answers -11.9 kJ/mol
The G
o
for a reaction A B at standard temperature is 10.0 kJ/mol. If the G for the reaction
under physiological concentrations of A and B is -1.2 kJ/mol, what is the approximate
concentration ratio
of B to A at standard temperature? correct answers 1/100
What is the thermodynamic driving force for the hydrophobic effect? correct answers The
entropy gained by releasing water molecules through sequestering hydrophobic molecules.
Tyrosine is soluble in water at 0.45mg/ml. Phenylalanine is soluble in water at 29.4mg/ml. What
possible explanation(s) would make sense? correct answers a. Tyrosine has an extra hydroxyl
group that could break up the organized water shell around it.
c. Phenylalanine is hydrophobic and would better support an organized water shell around it.
, Phosphoric acid has pKa 1 = 2.15, pKa 2 = 6.82 and pKa 3 = 12.38. At pH = 7.80, what
approximate
percentage of phosphoric acid is present as H2PO4-? correct answers 10
You have 1L of 1M phosphoric acid buffered at the pH from Question 9. How much 10M HCl
would
you need to add to change the pH to 6.80? Do not concern yourself with dilution effects. correct
answers 40ml
Identify the following amino acids: correct answers K, H, C
What pKa's are exhibited by these amino acids? correct answers 8.4, 6.0, 10.5
What is the identity of the following amino acid? correct answers L-Threonine
In which quadrant would you find beta-sheet structures? correct answers 1
Which amino acid would you anticipate the highest probability of finding in the alpha-helix left
region of the Ramachandran Diagram? correct answers Glycine
Hydrophobic interaction chromatography exploits the interaction between hydrophobic resin and
hydrophobic sidechains of peptides for separation. For the peptide with sequence
NFPDDDYKDEHF, what
pH would you predict for this peptide to be retained on the HPLC column most strongly? (Hint:
is this
peptide acidic or basic?) correct answers pH = 3
What is the fractional saturation (Y O2) for Hemoglobin and Myoglobin at pO2 = 40 torr? (Hint:
Order
What fundamental point about evolutionary processes is NOT correct? correct answers Variation
among individuals is directed.
What is the First Law of Thermodynamics? correct answers The total energy of any entire
system must be the same before and after an action or reaction.
What is the Second Law of Thermodynamics? correct answers S > 0 for spontaneous processes
What is the Equilibrium Gibbs Free Energy for a reaction at human physiological temperature
for a
reaction A B where, at equilibrium, [B] is 100 times that of [A]? correct answers -11.9 kJ/mol
The G
o
for a reaction A B at standard temperature is 10.0 kJ/mol. If the G for the reaction
under physiological concentrations of A and B is -1.2 kJ/mol, what is the approximate
concentration ratio
of B to A at standard temperature? correct answers 1/100
What is the thermodynamic driving force for the hydrophobic effect? correct answers The
entropy gained by releasing water molecules through sequestering hydrophobic molecules.
Tyrosine is soluble in water at 0.45mg/ml. Phenylalanine is soluble in water at 29.4mg/ml. What
possible explanation(s) would make sense? correct answers a. Tyrosine has an extra hydroxyl
group that could break up the organized water shell around it.
c. Phenylalanine is hydrophobic and would better support an organized water shell around it.
, Phosphoric acid has pKa 1 = 2.15, pKa 2 = 6.82 and pKa 3 = 12.38. At pH = 7.80, what
approximate
percentage of phosphoric acid is present as H2PO4-? correct answers 10
You have 1L of 1M phosphoric acid buffered at the pH from Question 9. How much 10M HCl
would
you need to add to change the pH to 6.80? Do not concern yourself with dilution effects. correct
answers 40ml
Identify the following amino acids: correct answers K, H, C
What pKa's are exhibited by these amino acids? correct answers 8.4, 6.0, 10.5
What is the identity of the following amino acid? correct answers L-Threonine
In which quadrant would you find beta-sheet structures? correct answers 1
Which amino acid would you anticipate the highest probability of finding in the alpha-helix left
region of the Ramachandran Diagram? correct answers Glycine
Hydrophobic interaction chromatography exploits the interaction between hydrophobic resin and
hydrophobic sidechains of peptides for separation. For the peptide with sequence
NFPDDDYKDEHF, what
pH would you predict for this peptide to be retained on the HPLC column most strongly? (Hint:
is this
peptide acidic or basic?) correct answers pH = 3
What is the fractional saturation (Y O2) for Hemoglobin and Myoglobin at pO2 = 40 torr? (Hint:
Order
