1. What infectious agent is the cause of "snuffles" in rabbits?
A. Mycoplasma pulmonis
B. Sendai virus
C. Bordetella bronchiseptica
D. Pasteurella multocida
Answer: D. Pasteurella multocida
Rationale: This bacterium is the most common cause of respiratory infections (“snuffles”) in
rabbits.
2. Which of the following is not one of the major clinical signs of inflammation?
A. Heat
B. Analgesia
C. Redness
D. Pain
Answer: B. Analgesia
Rationale: The classic signs of inflammation are heat, redness, swelling, pain, and loss of
function—not pain relief (analgesia).
3. The destruction of all organisms on an object is referred to as:
A. Sterilization
B. Disinfection
C. Pasteurization
D. Sanitization
Answer: A. Sterilization
Rationale: Sterilization eliminates all microorganisms, including spores.
4. Which of the following is not a type of animal facility design?
,A. Conventional
B. Barrier
C. VAF/SPF
D. Clean/Dirty
Answer: C. VAF/SPF
Rationale: VAF/SPF refers to animal health status, not facility design.
5. Which regulatory agency performs annual unannounced site inspections and enforces the
Animal Welfare Act?
A. AAALAC, Int.
B. FDA
C. PHS
D. USDA
Answer: D. USDA
Rationale: The USDA enforces the Animal Welfare Act through inspections.
6. ___________ pathology is the study of the structure of diseased tissues.
A. Topical
B. Clinical
C. Microscopic
D. Anatomical
Answer: D. Anatomical
Rationale: Anatomical pathology focuses on structural changes in diseased tissues.
7. Which types of animals are reared in open (non-filtered) cages and have an unknown
microbial status?
A. SPF
B. Germfree
C. Gnotobiotic
D. Conventional
Answer: D. Conventional
Rationale: Conventional animals are raised without controlled microbial environments.
,8. Which of the following warm-blooded vertebrates is not regulated by the Animal Welfare Act?
A. Horses used for antibody production
B. Sheep used in open heart surgery research
C. Pigeons used for behavioral testing
D. Wild rats used for viral infection
Answer: C. Pigeons used for behavioral testing
Rationale: Birds (like pigeons), rats, and mice bred for research are generally excluded from
AWA regulation.
1. ___________ is the science of poisons and the harmful or noxious effects these substances
have on living organisms.
A. Histology
B. Botany
C. Pathology
D. Toxicology
Answer: D. Toxicology
Rationale: Toxicology studies harmful effects of chemicals and poisons on living organisms.
2. If an animal dies spontaneously, changes in the tissues begin to occur as the body decomposes.
This process is called autolysis. What is the best way to preserve tissues for a diagnostic
necropsy?
A. Place the dead animal in a freezer
B. Place the animal in an autoclave
C. Place the animal in a refrigerator
D. Leave the animal in the exact location where it died
Answer: C. Place the animal in a refrigerator
Rationale: Refrigeration slows decomposition without damaging tissue like freezing.
3. What is the term used to describe the study of birth defects?
A. Teratology
B. Gynecology
C. Obstetrics
D. Mutitology
Answer: A. Teratology
Rationale: Teratology focuses on congenital abnormalities and developmental defects.
, 4. The written document that an investigator must complete, and submit to their Institutional
Animal Care and Use Committee, if they plan to use animals in research is called the
___________________.
A. Animal use protocol
B. Animal use grant
C. Research use protocol
D. Research use grant
Answer: A. Animal use protocol
Rationale: This document outlines ethical and procedural use of animals in research.
5. How long can bacteria survive when kept in the transport media of a sterile swab?
A. 24 hrs.
B. 48 hrs.
C. 72 hrs.
D. 96 hrs.
Answer: A. 24 hrs.
Rationale: Transport media typically preserves bacterial viability for up to 24 hours.
6. The word, "hypothyroidism", has the prefix _____, the suffix ________, and the root
__________.
A. ism; hypo; thyroid
B. hypo; ism; thyroid
C. thyroid; hypo; ism
D. hypo; thyroid; ism
Answer: B. hypo; ism; thyroid
Rationale: “Hypo-” (low), “thyroid” (root), “-ism” (condition).
7. A beagle is euthanized in May 1999. How long must the animal facility retain the USDA
identification tags (if used) on the animal for possible review by the USDA?
A. 6 months
B. 12 months
A. Mycoplasma pulmonis
B. Sendai virus
C. Bordetella bronchiseptica
D. Pasteurella multocida
Answer: D. Pasteurella multocida
Rationale: This bacterium is the most common cause of respiratory infections (“snuffles”) in
rabbits.
2. Which of the following is not one of the major clinical signs of inflammation?
A. Heat
B. Analgesia
C. Redness
D. Pain
Answer: B. Analgesia
Rationale: The classic signs of inflammation are heat, redness, swelling, pain, and loss of
function—not pain relief (analgesia).
3. The destruction of all organisms on an object is referred to as:
A. Sterilization
B. Disinfection
C. Pasteurization
D. Sanitization
Answer: A. Sterilization
Rationale: Sterilization eliminates all microorganisms, including spores.
4. Which of the following is not a type of animal facility design?
,A. Conventional
B. Barrier
C. VAF/SPF
D. Clean/Dirty
Answer: C. VAF/SPF
Rationale: VAF/SPF refers to animal health status, not facility design.
5. Which regulatory agency performs annual unannounced site inspections and enforces the
Animal Welfare Act?
A. AAALAC, Int.
B. FDA
C. PHS
D. USDA
Answer: D. USDA
Rationale: The USDA enforces the Animal Welfare Act through inspections.
6. ___________ pathology is the study of the structure of diseased tissues.
A. Topical
B. Clinical
C. Microscopic
D. Anatomical
Answer: D. Anatomical
Rationale: Anatomical pathology focuses on structural changes in diseased tissues.
7. Which types of animals are reared in open (non-filtered) cages and have an unknown
microbial status?
A. SPF
B. Germfree
C. Gnotobiotic
D. Conventional
Answer: D. Conventional
Rationale: Conventional animals are raised without controlled microbial environments.
,8. Which of the following warm-blooded vertebrates is not regulated by the Animal Welfare Act?
A. Horses used for antibody production
B. Sheep used in open heart surgery research
C. Pigeons used for behavioral testing
D. Wild rats used for viral infection
Answer: C. Pigeons used for behavioral testing
Rationale: Birds (like pigeons), rats, and mice bred for research are generally excluded from
AWA regulation.
1. ___________ is the science of poisons and the harmful or noxious effects these substances
have on living organisms.
A. Histology
B. Botany
C. Pathology
D. Toxicology
Answer: D. Toxicology
Rationale: Toxicology studies harmful effects of chemicals and poisons on living organisms.
2. If an animal dies spontaneously, changes in the tissues begin to occur as the body decomposes.
This process is called autolysis. What is the best way to preserve tissues for a diagnostic
necropsy?
A. Place the dead animal in a freezer
B. Place the animal in an autoclave
C. Place the animal in a refrigerator
D. Leave the animal in the exact location where it died
Answer: C. Place the animal in a refrigerator
Rationale: Refrigeration slows decomposition without damaging tissue like freezing.
3. What is the term used to describe the study of birth defects?
A. Teratology
B. Gynecology
C. Obstetrics
D. Mutitology
Answer: A. Teratology
Rationale: Teratology focuses on congenital abnormalities and developmental defects.
, 4. The written document that an investigator must complete, and submit to their Institutional
Animal Care and Use Committee, if they plan to use animals in research is called the
___________________.
A. Animal use protocol
B. Animal use grant
C. Research use protocol
D. Research use grant
Answer: A. Animal use protocol
Rationale: This document outlines ethical and procedural use of animals in research.
5. How long can bacteria survive when kept in the transport media of a sterile swab?
A. 24 hrs.
B. 48 hrs.
C. 72 hrs.
D. 96 hrs.
Answer: A. 24 hrs.
Rationale: Transport media typically preserves bacterial viability for up to 24 hours.
6. The word, "hypothyroidism", has the prefix _____, the suffix ________, and the root
__________.
A. ism; hypo; thyroid
B. hypo; ism; thyroid
C. thyroid; hypo; ism
D. hypo; thyroid; ism
Answer: B. hypo; ism; thyroid
Rationale: “Hypo-” (low), “thyroid” (root), “-ism” (condition).
7. A beagle is euthanized in May 1999. How long must the animal facility retain the USDA
identification tags (if used) on the animal for possible review by the USDA?
A. 6 months
B. 12 months
