ASCP BOARD OF CERTIFICATION MLT BLOOD BANK
PRACTICE TEST LEVEL 1–6 COMPREHENSIVE STUDY
GUIDE & PRACTICE QUESTIONS (2026) GRADED A+
Chloroquine diphosphate can be used in blood banking for which of the following
methodologies? A: To remove antibody bound to red cells so that cells can be
further tested
B: To remove a specific antibody in serum or
plasma
C: To remove ABO antigens from cells
D: To remove Rh antigens from cells
Correct Answer - A
Which of the following transfusion reactions can a diagnosis be more firmly established
by evaluating BNP levels before & after transfusion?
A: TACO
B: Delayed hemolytic transfusion
reactions
C: Transfusion associated sepsis
D: Allergic Transfusion Reactions
Correct Answer – A
A blood donor has totype: hh, AB. ing anti-A and anti-B antisera., the donor's red cells
will type as group US? - ANSWER - O
which of the following is proper procedure for preparation of platelets from Whole
blood? - ANSWER - light spin followed by a hard spin
pg. 1
,The minimum hemoglobin concentration in a finger-stick from a male blood donor is: -
ANSWER - 13.0 g/dL
In which system are DR antigens are
found? A: Kell
B: HLA
C:
Duffy
D:
ABO
Correct Answer - B
All of the following are the reacting substances that may be in the coombs sera
(antihuman globulin) EXCEPT?
A: Monoclonal antibody
sources B: Polyclonal
antibody sources
C: Complement antibodies (ex:
anti-C3b) D: Anti-IgM sources
Correct Answer - D
The DAT is used to detect:
A:Antibodies sensitizing red
cells B: Antibodies in the
plasma
C: Antigens coating red
cells D: Antigens in the
plasma
pg. 2
,Correct Answer - A
When collecting routine blood product donations, the venipuncture site first
cleaned with: A: 70% Isopropyl alcohol
B: 50% Iodine
C: 0.7% Iodophor
compound D: 2%
Chlorhexidine
Correct Answer - C
A sample has reactions occurring at immediate spin & AHG i a panel that show varying
reaction
strengths. There is NO obvious pattern that matches a particular panel cell or single
antigen profile & the auto-control was negative. Which of the following is the most likely
cause?
A: An IgM & IgG
antibody B: An IgG
antibody ONLY C: An
IgM antibody ONLY
D: an autoantibody
Correct Answer - A
Red cells unit containing CPDA-1 as an anticoagulant-preservative may be stored for how
long prior to transfusion?
A: 5 days
B: 15 days
C: 25 days
D: 35 days
Correct Answer - D
The lectin Dolichus biflorus agglutinates which
pg. 3
, of cells? A: B
B:
A1B
C:
A2
D: Both A1B & A2
Correct Answer - B
Which of the following antigen groups is closely related to the ABO antigens on the red
cell membrane? A: Rh
B:
Kell
C: I,
i
D: Duffy
Correct Answer - C
In an extreme emergency, if the ABO/Rh type are unknown, which of the following
should be given to the patient?
A: Group O,
Rh+ B: Group
AB, Rh-C:
Group O, Rh-
D: Any blood type is OK
Correct Answer - C
How much time must elapse between apheresis RBC
donations? A: 48 hours
B: 7 days
pg. 4
PRACTICE TEST LEVEL 1–6 COMPREHENSIVE STUDY
GUIDE & PRACTICE QUESTIONS (2026) GRADED A+
Chloroquine diphosphate can be used in blood banking for which of the following
methodologies? A: To remove antibody bound to red cells so that cells can be
further tested
B: To remove a specific antibody in serum or
plasma
C: To remove ABO antigens from cells
D: To remove Rh antigens from cells
Correct Answer - A
Which of the following transfusion reactions can a diagnosis be more firmly established
by evaluating BNP levels before & after transfusion?
A: TACO
B: Delayed hemolytic transfusion
reactions
C: Transfusion associated sepsis
D: Allergic Transfusion Reactions
Correct Answer – A
A blood donor has totype: hh, AB. ing anti-A and anti-B antisera., the donor's red cells
will type as group US? - ANSWER - O
which of the following is proper procedure for preparation of platelets from Whole
blood? - ANSWER - light spin followed by a hard spin
pg. 1
,The minimum hemoglobin concentration in a finger-stick from a male blood donor is: -
ANSWER - 13.0 g/dL
In which system are DR antigens are
found? A: Kell
B: HLA
C:
Duffy
D:
ABO
Correct Answer - B
All of the following are the reacting substances that may be in the coombs sera
(antihuman globulin) EXCEPT?
A: Monoclonal antibody
sources B: Polyclonal
antibody sources
C: Complement antibodies (ex:
anti-C3b) D: Anti-IgM sources
Correct Answer - D
The DAT is used to detect:
A:Antibodies sensitizing red
cells B: Antibodies in the
plasma
C: Antigens coating red
cells D: Antigens in the
plasma
pg. 2
,Correct Answer - A
When collecting routine blood product donations, the venipuncture site first
cleaned with: A: 70% Isopropyl alcohol
B: 50% Iodine
C: 0.7% Iodophor
compound D: 2%
Chlorhexidine
Correct Answer - C
A sample has reactions occurring at immediate spin & AHG i a panel that show varying
reaction
strengths. There is NO obvious pattern that matches a particular panel cell or single
antigen profile & the auto-control was negative. Which of the following is the most likely
cause?
A: An IgM & IgG
antibody B: An IgG
antibody ONLY C: An
IgM antibody ONLY
D: an autoantibody
Correct Answer - A
Red cells unit containing CPDA-1 as an anticoagulant-preservative may be stored for how
long prior to transfusion?
A: 5 days
B: 15 days
C: 25 days
D: 35 days
Correct Answer - D
The lectin Dolichus biflorus agglutinates which
pg. 3
, of cells? A: B
B:
A1B
C:
A2
D: Both A1B & A2
Correct Answer - B
Which of the following antigen groups is closely related to the ABO antigens on the red
cell membrane? A: Rh
B:
Kell
C: I,
i
D: Duffy
Correct Answer - C
In an extreme emergency, if the ABO/Rh type are unknown, which of the following
should be given to the patient?
A: Group O,
Rh+ B: Group
AB, Rh-C:
Group O, Rh-
D: Any blood type is OK
Correct Answer - C
How much time must elapse between apheresis RBC
donations? A: 48 hours
B: 7 days
pg. 4
