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AORN Periop 101 Exam 2026/2027 | 150+ Questions & Answers | Asepsis, OR Safety, Anesthesia, Sterilization & Patient Care | AORN

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This comprehensive AORN Periop 101 Exam 2026/2027 study document contains 150+ exam questions and answers covering the essential principles of perioperative nursing and safe operating-room practice. Across 56 pages, the material addresses surgical conscience, aseptic and sterile technique, surgical hand antisepsis, OR attire and restricted zones, the Universal Protocol and Time Out, patient identification, informed consent, medication safety, surgical counts, specimen management, wound classification, skin preparation, surgical site infection prevention, patient positioning, pressure-injury prevention, environmental cleaning, sterilization, decontamination, and sterile storage. The document also provides extensive preparation in general, regional, local and moderate anesthesia; malignant hyperthermia and dantrolene; electrosurgical safety; surgical fire prevention; minimally invasive surgery; insufflation; powered instruments; sutures and needles; hemostasis; drains; wound healing; latex allergy; blood-borne pathogen prevention; postoperative care; PACU assessment; SBAR communication; and perioperative documentation. Questions include both single-best-answer and select-all-that-apply formats, making the resource useful for active recall and exam-focused revision. The content corresponds closely with the professional scope of Periop 101: A Core Curriculum, which AORN describes as an evidence-based program designed to prepare novice nurses for the operating room. The official OR curriculum combines online and hands-on education and is developed using the latest AORN Guidelines for Perioperative Practice. AORN reports that the OR learning plan contains 23 online modules and incorporates graduate-level Quality and Safety Education for Nurses (QSEN) competencies. A key reference for this subject is the AORN, Guidelines for Perioperative Practice, 2026 Edition. According to AORN, the 2026 edition contains 36 evidence-based guidelines, with important updates involving transmission-based precautions, pneumatic tourniquet safety, autologous tissue management, instrument cleaning, surgical energy devices, and care of patients receiving local-only anesthesia. These evidence-based guidelines provide the professional foundation for contemporary perioperative practice and the Periop 101 curriculum. Relevant Students: This material is particularly relevant for novice perioperative RNs, registered nurses transitioning into the operating room, Periop 101 learners, OR nurses, circulating nurses, scrub-role nurses, ambulatory surgery nurses, nursing students studying perioperative care, and nurses reviewing evidence-based surgical safety practices. AORN specifically identifies Periop 101 OR as preparation for novice nurses entering the operating room, while its broader perioperative educational resources also support nurses working across preoperative, intraoperative and postoperative settings. Keywords: AORN Periop 101 exam, AORN Periop 101 exam 2026, Periop 101 exam , AORN Periop 101 questions and answers, Periop 101 practice questions, perioperative nursing exam, perioperative nursing questions and answers, operating room nursing, OR nurse exam, surgical asepsis, sterile technique, surgical conscience, AORN guidelines, Universal Protocol, surgical Time Out, surgical site infection prevention, patient positioning, anesthesia nursing, malignant hyperthermia, dantrolene, electrosurgical safety, OR fire safety, surgical counts, retained surgical items, sterilization and decontamination, surgical skin preparation, wound classification, wound healing, surgical instruments, perioperative patient assessment, informed consent, PACU nursing, SBAR communication, perioperative documentation, environmental cleaning, infection prevention, surgical nursing study guide, perioperative nurse training

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AORN Periop 101 Exam
2026/2027 Exam Questions and
Answers | Already Graded A+



When applying the concept of surgical conscience which of the following

statement should be considered? Select all that apply. - ANSWER

✔✔A. It involves mental discipline & the ability to speak out


B. It is essential to the delivery of optimal care and prevent harm.

D. Surgical conscience allows for no compromise in the principles of

aseptic technique.

,In preparing for a regulatory survey, your quality consultant questions

you about the restricted zone in your OR suite. The following statements

are true: - ANSWER ✔✔A. Air flow in the OR rooms should be

cleaned by HEPA filters.

C. Mask are required when open sterile supplies are present.

E. Temperature is maintained between 68 and 75 degree

F.

You are assigned to relieve a surgical technologist for lunch. You will

need: - ANSWER ✔✔A. A sterile gown and gloves opened on a table

(other than the main instrument table) for closed gloving.

The Joint Commission Universal Protocol consists of: Select all that

apply. - ANSWER ✔✔A. Preoperative verification


B. Surgical site marking

C. Time Out

Which of the following procedures reflects best practice for transferring a

medication vial to the sterile field? - ANSWER ✔✔D. Use a sterile

transfer device

,Which of the following practices is recommended for handling of natural

absorbable suture? - ANSWER ✔✔C. Open the package carefully

over a small basin to prevent splashing of the packaging solution

Recommended sponge count procedures include all of the following

EXCEPT? - ANSWER ✔✔B. Counting silently so it doesn't distract

other personnel.


What is the correct definition of incisional biopsy? - ANSWER ✔✔D. A

small portion of tissue is incised and sent for examination.

Which of the following methods listed below may prevent mishap, event,

or fire from occurring when handling electrosurgical equipment? Select

all that apply. - ANSWER ✔✔A. Allow enough time for alcohol based

skin preparations to completely dry.

B. Do not use in an oxygen-enriched environment, such as the trachea

when oxygen is being "blown over" the patient.

D. Visually check the integrity and insulation of all cords and coagulation

instruments before use.

Intrinsic and extrinsic factors that may lead to development of

preoperative pressure ulcer include: Select all that apply. - ANSWER

✔✔A. Duration of the procedure


COPYRIGHT©PROFFKERRYMARTIN 2025/2026. YEAR PUBLISHED 2026. COMPANY REGISTRATION NUMBER: 619652435. TERMS OF USE.
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, B. Exaggerated positions

C. Circulatory disorders

D. Malnutrition

Which of the following statements reflect the concept of critical thinking?

Select all that apply. - ANSWER ✔✔C. Informed practice


D. Holistic thinking

E. Unbiased thinking

F. Reasoned application of a step by step process

What is the best drape to use for an umbilical hernia repair? -

ANSWER ✔✔B. Laparotomy


Which of the following statements correctly describes recommendations

for preoperative patient skin antisepsis? - ANSWER ✔✔D. Prep

should start at the site of the incision and move outwards.

What are the 3 main factors to consider when assessing the patient for

allergies? - ANSWER ✔✔C. Prior reaction, heredity, type of reaction


The following steps can be used to reduce personnel exposure to

specimen fixatives such as formalin. Select all that apply. - ANSWER

✔✔A. Avoid splashing while pouring

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