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Scym Ascp Review Tips Questions And Answers Sure A.pdf

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SCYM ASCP REVIEW TIPS QUESTIONS AND ANSWERS SURE A.pdf

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SCYM ASCP REVIEW TIPS QUESTIONS AND
ANSWERS SURE A+
✔✔ Adherent Samples - ✔✔Cell lines, some tumors

✔✔Liquid Samples - ✔✔Blood, spleen, bone marrow, fresh marine water

✔✔Solid Tissue Samples - ✔✔Tumors, organs, plants

✔✔Cons of enzymatic disruption methods - ✔✔Can strip surface epitopes

✔✔How to prevent coagulation - ✔✔ACD, EDTA, heparin, or physically disrupt cells
using a method such as vortexing

✔✔How long can whole blood remain at room temperature? - ✔✔24 hours, then
refrigerate for up to 72 hours

✔✔Cells that are too large for flow cytometry - ✔✔Cardiomyocytes and Megakaryocytes

✔✔Factors to consider when fixing to preserve the epitope - ✔✔Fixative type, fixation
time, temperature, fluorochrome compatibility, surface marker compatibility

✔✔Alcohol fixation cannot be used on these fluorochromes - ✔✔PE and GFP

✔✔Aldehydes - ✔✔formaldehyde, increases background, destroys epitopes, 4%
permeabilizes

✔✔Alcohol - ✔✔Ethanol and methanol permeabilizes, alters morphology, nuclear, cell
cycle

✔✔Harsh Detergents - ✔✔Triton X-100, NP-40

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