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MBIO 3401 LAB FINAL QUESTIONS AND ANSWERS 202

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MBIO 3401 LAB FINAL QUESTIONS AND ANSWERS 202

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MBIO 3401 LAB FINAL QUESTIONS AND ANSWERS 2026




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1 of 50

Term



EMB Bacteria



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Eosin Methylene Blue helps ID E. Coli. If microbes is able to ferment lactose,
it'll produce green metalic sheath (e. coli species), if not able to ferment
lactose it appears as dark green/black in color.




Detects the production of acetoin from fermentation of glucose.

Positive: Red Color after addition of reagents.


Negative: No color change, suggesting organism either didn't produce acetoin or
low quantity.

, Helps determine if a bacterium's' ability to reduce nitrate NO3- to nitrite NO2- or
other nitrogenous compounds.


Positive test: Red Coloration from reaection of nitrate added.


Negative test: No color change.


If microbe can ferment lactose, it changes media to yellow color. If unable too, it
remains as red.


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2 of 50

Definition



Quorum sensing



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How is the Zone of Inhibition related How do the bacterial cell
to the Antibiotic Effectiveness? communicate with each other?




How do you make a bacterial cell
What is the process of making beer?
competent?


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3 of 50

,Definition



CDC & WHO

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What if Nitrate remains unaltered? Branch that studies Fungi



Agencies involved in disease
How does infectious agent transmit?
control


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4 of 50

Term



HEA Bacteria Differentiation



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If able to ferment one of the compounds of lactose, sucrose, and slicin then
medium turns yellow to orange indicating acidic pH change. If not able to
ferment then no color change is visible.




Detects the production of acetoin from fermentation of glucose.

Positive: Red Color after addition of reagents.

, Negative: No color change, suggesting organism either didn't produce acetoin or
low quantity.

Antiseptics are more applied on living tissues such as the skin, whereas disinfectants
are more applied towards destroying microbes in areas such as bathrooms and can
cause to living tissues.




If microbe can ferment lactose, it changes media to yellow color. If unable too, it
remains as red.


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5 of 50

Term



What is the purpose of the IMViC Test?



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This test is used to identify
Water, malted barley, hops, and yeast
enteric bacteria such as
(Saccharomyces)
pathogens.




Cooking
Fermenting Study of microorganisms that inhabit,
Bottling create, or contaminate food
Carbonating


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