bio97 midterm 1 Exam Questions and
Answers with Verified Solutions | Latest
Updated 2026
which nitrogenous bases are adenine and guanine
purines?
which nitrogenous bases are thymine, cytosine, and uracil
pyrimidines?
adenine and thymine make _____ 2 hydrogen
bonds
cytosine and guanine make ____ 3 hydrogen
bonds
a nucleoside is made of sugar and nitrogenous base
a nucleotide is made of phosphate group, sugar, and nitrogenous
base
nucleotides are linked together by phosphodiester bonds
phosphodiester bonds are linked 3' OH of the sugar and the O- of the
by phosphate
the ____ group
, phosphodiester bonds are formed dehydration synthesis
through ______
minor grooves of a dna double small
helix
have a ____ amount of
nucleotides
major grooves of a dna double large
helix
have a _____ amount of
nucleotides
to replicate dna, what is needed? dna polymerase, template strand, primer,
and
nucleotides
what are the 3 steps of PCR and 1. denaturing (95 degrees C)
at 2. annealing (50 degrees C)
what temperatures do these steps 3. extension (72 degrees C)
happen?
what is the difference between a a dNTP has a OH group at the 3' end and
dNTP and ddNTP? a ddNTP
has a H at the 3' end; ddNTP is used to
terminate
replication
in cells, what is used as a primer? RNA
in PCR, what is used as a primer? DNA
Answers with Verified Solutions | Latest
Updated 2026
which nitrogenous bases are adenine and guanine
purines?
which nitrogenous bases are thymine, cytosine, and uracil
pyrimidines?
adenine and thymine make _____ 2 hydrogen
bonds
cytosine and guanine make ____ 3 hydrogen
bonds
a nucleoside is made of sugar and nitrogenous base
a nucleotide is made of phosphate group, sugar, and nitrogenous
base
nucleotides are linked together by phosphodiester bonds
phosphodiester bonds are linked 3' OH of the sugar and the O- of the
by phosphate
the ____ group
, phosphodiester bonds are formed dehydration synthesis
through ______
minor grooves of a dna double small
helix
have a ____ amount of
nucleotides
major grooves of a dna double large
helix
have a _____ amount of
nucleotides
to replicate dna, what is needed? dna polymerase, template strand, primer,
and
nucleotides
what are the 3 steps of PCR and 1. denaturing (95 degrees C)
at 2. annealing (50 degrees C)
what temperatures do these steps 3. extension (72 degrees C)
happen?
what is the difference between a a dNTP has a OH group at the 3' end and
dNTP and ddNTP? a ddNTP
has a H at the 3' end; ddNTP is used to
terminate
replication
in cells, what is used as a primer? RNA
in PCR, what is used as a primer? DNA
