ANSC446 Midterm Exam Questions with 100%
Correct Answers
The primary chemical structure of double-stranded DNA includes
phosphodiester linkage in the deoxyribose backbone
Nucleotides are monomers of DNA. What does each nucleotide consist of?
A pruine or pyrimidine base, a phosphate group, and deoxyribose
The most important molecule for long-term storage of genetic information is
deoxyribonucleic acid
Which of the statement is NOT true about DNA replication
DNA ligase works the same way as DNA polymerase I to add dNTP to the new strand
Which of the following is NOT part of post-transcriptional process in eukaryote
Binding of transcription factors to the promoter sequence
DNA promoter is defined as
a specific DNA sequence located at 5' flanking region of a gene and interactive with RNA
polymerase or transcription factor
TO clone insulin cDNA sequence into a plasmid at the Hind III restriction enzyme site
(AAGCTT), you will need to cut the plasmid with BAM HI and to prepare the insulin cDNA with
the necessary complementary ends of Hind III sequence. Will this approach work in the lab?
No
, Which statement is WRONG about early stage of animal development?
Before blastocyst stage, embryo undergoes dramatic movement and formation of three cell
layers (ectoderm, endoderm, mesoderm)
Gene expression can be defined as
transcription of DNA to mRNA, which is used as a template for protein synthesis
Which statement is WRONG about embryonic stem cells
What are the Okazaki fragments?
Short fragment of the newly synthesized DNA
The conversation of of a double-stranded DNA molecule to single strands (denature) and the
reversal of the that process are strongly dependent on
the temperature of the DNA solution
One of the steps in construction of a genomic DNA library is to digest the DNA with restriction
enzymes, followed by DNA ligation to a vector
True
DNA transcription is the process of making another copy of double strand DNA
False
Animal cloning is experimented by injected fetal fibroblast cell (or nucleus) to enucleated
mature ovum
Correct Answers
The primary chemical structure of double-stranded DNA includes
phosphodiester linkage in the deoxyribose backbone
Nucleotides are monomers of DNA. What does each nucleotide consist of?
A pruine or pyrimidine base, a phosphate group, and deoxyribose
The most important molecule for long-term storage of genetic information is
deoxyribonucleic acid
Which of the statement is NOT true about DNA replication
DNA ligase works the same way as DNA polymerase I to add dNTP to the new strand
Which of the following is NOT part of post-transcriptional process in eukaryote
Binding of transcription factors to the promoter sequence
DNA promoter is defined as
a specific DNA sequence located at 5' flanking region of a gene and interactive with RNA
polymerase or transcription factor
TO clone insulin cDNA sequence into a plasmid at the Hind III restriction enzyme site
(AAGCTT), you will need to cut the plasmid with BAM HI and to prepare the insulin cDNA with
the necessary complementary ends of Hind III sequence. Will this approach work in the lab?
No
, Which statement is WRONG about early stage of animal development?
Before blastocyst stage, embryo undergoes dramatic movement and formation of three cell
layers (ectoderm, endoderm, mesoderm)
Gene expression can be defined as
transcription of DNA to mRNA, which is used as a template for protein synthesis
Which statement is WRONG about embryonic stem cells
What are the Okazaki fragments?
Short fragment of the newly synthesized DNA
The conversation of of a double-stranded DNA molecule to single strands (denature) and the
reversal of the that process are strongly dependent on
the temperature of the DNA solution
One of the steps in construction of a genomic DNA library is to digest the DNA with restriction
enzymes, followed by DNA ligation to a vector
True
DNA transcription is the process of making another copy of double strand DNA
False
Animal cloning is experimented by injected fetal fibroblast cell (or nucleus) to enucleated
mature ovum
