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Testbank: BIO 181 Exam 2 2026 – Comprehensive Questions & Verified Correct Answers

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Excel on your BIO 181 Exam 2 with this comprehensive test bank featuring actual exam-style questions and verified correct answers, updated for the 2026–2027 academic year. This complete resource covers the core topics typically tested in the second exam for General Biology I, including cellular respiration, photosynthesis, molecular biology (DNA replication, transcription, translation), and gene expression, and is relevant for courses at institutions such as Arizona State University and Grand Canyon University .

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BIO 181 Exam 2 Questions and
Correct Answers (Verified Answers) |
New 2026/27 Update | Graded A+

RNA polymerase binds to promoter, RNA is synthesized in the 5' --> 3' direction, The
new mRNA is released, RNA polymerase reaches the terminator, DNA is unwound,
forming an open complex - answer- Place the following events of transcription in the
correct order.

None of them

Explanation
The two enzymes that operate at extreme temperatures are least likely to be found
within a mammal. There are regions of low pH in mammals and so enzymes that have
optimal activity under acidic conditions could function in a mammal but there are no
regions in mammals that are maintained at extreme temperatures. - answer- Which two
enzymes are least likely to be functional within a mammalian organism? (Choose two).

The enthalpy of a reaction is not affected directly by temperature.

If a reaction has a negative change in free energy then it will be exothermic.

An increase in disorder is a form of entropy. - answer- Drag the correct word from the
left into the sentence on the right to complete each sentence.

kinetic energy
entropy
free energy
enthalpy

Energetic molecules such as NADH and ATP are often reactants of anabolic reactions.

Energetic molecules such as NADH and ATP are often products of catabolic reactions.

In the reaction Pyruvate + NADH -> Lactate + NAD+, pyruvate is being reduced, while
NADH is being oxidized. - answer- Drag the correct word from the left into the sentence
on the right to complete each sentence.

oxidized

,reduced
anabolic
catabolic

1.) Glycolysis
2.) Breakdown of pyruvate
3.) Citric acid cycle
4.) Oxidative phosphorylation - answer- Place the cellular respiration pathways in their
correct locations.

Citric acid cycle
Breakdown of pyruvate
Glycolysis
Oxidative phosphorylation

1.) NADH
2.) NAD+
3.) 2H⁺ + 1/2 O₂
4.) H20 - answer- Place the name of the molecule in the proper location within the
electron transport chain.

NAD-
H20
NADH
2H- + 1/2 O2

Top to bottom and left to right

1.) Proteins, Amino Acids
2.) Carbohydrates, Sugars
3.) Fats, Glycerol, Fatty Acids - answer- Cellular respiration is fueled by all types of
macromolecules. Place the molecules in the appropriate pathways to show how ATP
can be derived from all types of food taken in from the diet.

Amino Acids
Sugars
Fatty Acids
Glycerol
Proteins
Fats
Carbohydrates

Energy Investment, Cleavage, Energy Liberation - answer- Label the phases of
glycosis.

Energy Liberation

, Energy Investment
Cleavage

TOP: D, A
BOTTOM: B, C, E - answer- Match each molecule in the citric acid cycle with its
location.

Top to bottom & left to right:

Intermembrane Space
Outer membrane
Inner Membrane
Thylakoid
Granum
Stroma - answer- Match the label to the correct structure on the chloroplast.

Inner membrane
Intermembrane Space
Outer membrane
Granum
Stroma
Thylakoid

TOP RIGHT: Carbon Fixation
MIDDLE LEFT: Regeneration of RuBP
BOTTOM RIGHT: Reduction and carbohydrate production - answer- Place the stages of
the Calvin cycle in the correct order of events.

Carbon Fixation
Regeneration of RuBP
Reduction and carbohydrate production

TOP: ATP, ADP, NADP+, NADPH
MIDDLE: H20, C02
BOTTOM: O2, Glucose - answer- Indicate the correct location of each molecule in the
light reaction and Calvin cycle.

ADP
NADPH
H20
NADP+
ATP
O2
CO2
Glucose

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