MICROBIOLOGY -PAPER 1
General
Introduction and Bacterial Taxonomy
SN
1. Robert Koch*** - four contributions**, Koch's Postulates - discuss
2. Eukaryotes and Prokaryotes - four differences
3. Louise Pasteur - contributions in Microbiology
Morphology and Physiology of Bacteria
SN
1. Types of Microscopes - enumerate, Dark Ground Microscope
2. Bacterial Growth Curve** - diagram
3. Bacterial Spore*
4. [Bacterial Capsule - describe, Capsulated Bacteria - name two, Detection of
Capsule - two methods]*
5. Cell Wall of Gram Positive Organisms - describe, Functions of Cell Wall
6. Bacterial Flagella - define, types with examples, demonstration - two methods
LAQ
1. Bacterial Cell Wall - structure and function
Sterilization and Disinfection
SN
1. Gaseous Disinfectants - describe with uses
2. Tyndallisation - define, principle, when is it used
LAQ
1. Sterilization and disinfection - define****, enumerate methods*, Dry Heat
sterilization - enumerate methods*, two methods with principles, Hot Air Oven -
role in sterilization; Autoclave** - principle**, types, applications*, working,
operational complications, diagram, four items sterilized in autoclave; Moist Heat
Sterilization - enumerate methods
2. Four Chemical Agents used for Disinfection, Properties of an Ideal Disinfectant
Culture Media
SN
Faizan Khan
, 1. Culture Media - classify* with examples, Enriched Media - examples; Selective
Media
2. Enrichment Media* - describe with two examples; How it differs from Enriched
Media, Solid Culture Media without Agar - two examples
Bacterial Genetics
SN
1. Mutational and Plasmid-mediated (Transferable) drug resistance*** -four differences, six
differences
2. Transduction
3. Conjugation
4. Mutation - define [sub-question]
LAQ
1. Gene Transfer in Bacteria - enumerate methods, any one in detail
General Virology
General Properties of Virus
SN
1. Egg Inoculation Method for Viral Culture
2. Methods of detecting viral growth in cell cultures/tissue cultures*** - different methods
of observing growth of cell lines
3. Stages of Viral Multiplication
4. Embryonated Hen's Egg* - cross section, routes of inoculation, one example of each
route; uses, Enumerate various methods of cultivation of viruses
Virus-Host Interactions
SN
1. Negri bodies - short note
2. Inclusion Bodies**** - short note, mention two intracytoplasmic and two intranuclear
inclusion bodies
General Mycology
General Aspects
SN
1. [sub-question] Classify medically important Fungi
2. Classify Fungi, one example each*; give morphological classification
LAQ
1. [sub-question] Morphological Classification of Fungi*, one example of each
Unspecified
Faizan Khan
General
Introduction and Bacterial Taxonomy
SN
1. Robert Koch*** - four contributions**, Koch's Postulates - discuss
2. Eukaryotes and Prokaryotes - four differences
3. Louise Pasteur - contributions in Microbiology
Morphology and Physiology of Bacteria
SN
1. Types of Microscopes - enumerate, Dark Ground Microscope
2. Bacterial Growth Curve** - diagram
3. Bacterial Spore*
4. [Bacterial Capsule - describe, Capsulated Bacteria - name two, Detection of
Capsule - two methods]*
5. Cell Wall of Gram Positive Organisms - describe, Functions of Cell Wall
6. Bacterial Flagella - define, types with examples, demonstration - two methods
LAQ
1. Bacterial Cell Wall - structure and function
Sterilization and Disinfection
SN
1. Gaseous Disinfectants - describe with uses
2. Tyndallisation - define, principle, when is it used
LAQ
1. Sterilization and disinfection - define****, enumerate methods*, Dry Heat
sterilization - enumerate methods*, two methods with principles, Hot Air Oven -
role in sterilization; Autoclave** - principle**, types, applications*, working,
operational complications, diagram, four items sterilized in autoclave; Moist Heat
Sterilization - enumerate methods
2. Four Chemical Agents used for Disinfection, Properties of an Ideal Disinfectant
Culture Media
SN
Faizan Khan
, 1. Culture Media - classify* with examples, Enriched Media - examples; Selective
Media
2. Enrichment Media* - describe with two examples; How it differs from Enriched
Media, Solid Culture Media without Agar - two examples
Bacterial Genetics
SN
1. Mutational and Plasmid-mediated (Transferable) drug resistance*** -four differences, six
differences
2. Transduction
3. Conjugation
4. Mutation - define [sub-question]
LAQ
1. Gene Transfer in Bacteria - enumerate methods, any one in detail
General Virology
General Properties of Virus
SN
1. Egg Inoculation Method for Viral Culture
2. Methods of detecting viral growth in cell cultures/tissue cultures*** - different methods
of observing growth of cell lines
3. Stages of Viral Multiplication
4. Embryonated Hen's Egg* - cross section, routes of inoculation, one example of each
route; uses, Enumerate various methods of cultivation of viruses
Virus-Host Interactions
SN
1. Negri bodies - short note
2. Inclusion Bodies**** - short note, mention two intracytoplasmic and two intranuclear
inclusion bodies
General Mycology
General Aspects
SN
1. [sub-question] Classify medically important Fungi
2. Classify Fungi, one example each*; give morphological classification
LAQ
1. [sub-question] Morphological Classification of Fungi*, one example of each
Unspecified
Faizan Khan
