Please check the examination details below before entering your candidate information
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 1/Level 2 GCSE (9–1)
Monday 8 June 2026
Morning (Time: 1 hour 45 minutes) Paper
reference 1BI0/2F
Biology
PAPER 2
Foundation Tier
You must have: Total Marks
Ruler, calculator
Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 100.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• toIn questions marked with an asterisk (*), marks will be awarded for your ability
structure your answer logically, showing how the points that you make are
related or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
P79088A
©2026 Pearson Education Ltd.
P:1/1/1/1/1/1/1/1/
*P79088A0136*
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 The enzyme amylase digests starch.
Figure 1 shows some of the equipment used to investigate the effect of pH
on amylase.
pipette
spotting tile
mixture of amylase
solution and pH 7 drop of
buffer solution starch iodine solution
solution
test tube 1 test tube 2
Figure 1
(a) (i) Draw one straight line from each stage of the investigation to the reason for
the stage.
(2)
Stage of investigation Reason
to make sure the solutions
are at the same temperature
putting test tubes 1 and 2 in to make sure that the pH is
a water bath, before mixing kept constant
them together
to see if starch is still present
putting some of the enzyme
and starch mixture onto the to see if iodine is affected
iodine solution by pH
to see if the enzyme
contains glucose
2
*P79088A0236*
, (ii) Name the extra piece of equipment needed to make sure that the mixture
was tested every ten seconds.
(1)
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(iii) Figure 2 shows the results when the mixture was at pH 7.
Figure 2
State the time taken for all the starch in the mixture to be digested by the
amylase at pH 7.
(1)
.............................................................. seconds
3
*P79088A0336* Turn over
, (iv) The optimum pH for amylase to digest starch is pH 7.
Figure 3 shows a diagram of amylase at pH 7 and pH 3 and part of a
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
starch molecule.
amylase
at pH 7
part of starch
molecule
amylase
at pH 3
Figure 3
Explain the effect pH 3 will have on the activity of amylase.
(3)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 7 marks)
4
*P79088A0436*
Candidate surname Other names
Centre Number Candidate Number
Pearson Edexcel Level 1/Level 2 GCSE (9–1)
Monday 8 June 2026
Morning (Time: 1 hour 45 minutes) Paper
reference 1BI0/2F
Biology
PAPER 2
Foundation Tier
You must have: Total Marks
Ruler, calculator
Instructions
•• Use black ink or ball-point pen.
Fill in the boxes at the top of this page with your name,
centre number and candidate number.
•• Answer all questions.
Answer the questions in the spaces provided
– there may be more space than you need.
Information
•• The total mark for this paper is 100.
The marks for each question are shown in brackets
– use this as a guide as to how much time to spend on each question.
• toIn questions marked with an asterisk (*), marks will be awarded for your ability
structure your answer logically, showing how the points that you make are
related or follow on from each other where appropriate.
Advice
•• Read each question carefully before you start to answer it.
Try to answer every question.
• Check your answers if you have time at the end.
Turn over
P79088A
©2026 Pearson Education Ltd.
P:1/1/1/1/1/1/1/1/
*P79088A0136*
, Answer ALL questions. Write your answers in the spaces provided.
Some questions must be answered with a cross in a box . If you change your mind about an
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
answer, put a line through the box and then mark your new answer with a cross .
1 The enzyme amylase digests starch.
Figure 1 shows some of the equipment used to investigate the effect of pH
on amylase.
pipette
spotting tile
mixture of amylase
solution and pH 7 drop of
buffer solution starch iodine solution
solution
test tube 1 test tube 2
Figure 1
(a) (i) Draw one straight line from each stage of the investigation to the reason for
the stage.
(2)
Stage of investigation Reason
to make sure the solutions
are at the same temperature
putting test tubes 1 and 2 in to make sure that the pH is
a water bath, before mixing kept constant
them together
to see if starch is still present
putting some of the enzyme
and starch mixture onto the to see if iodine is affected
iodine solution by pH
to see if the enzyme
contains glucose
2
*P79088A0236*
, (ii) Name the extra piece of equipment needed to make sure that the mixture
was tested every ten seconds.
(1)
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(iii) Figure 2 shows the results when the mixture was at pH 7.
Figure 2
State the time taken for all the starch in the mixture to be digested by the
amylase at pH 7.
(1)
.............................................................. seconds
3
*P79088A0336* Turn over
, (iv) The optimum pH for amylase to digest starch is pH 7.
Figure 3 shows a diagram of amylase at pH 7 and pH 3 and part of a
DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA DO NOT WRITE IN THIS AREA
starch molecule.
amylase
at pH 7
part of starch
molecule
amylase
at pH 3
Figure 3
Explain the effect pH 3 will have on the activity of amylase.
(3)
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
. . . . . . . . . . . .. .. .. .. .. .. .. .. .. .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ............................................................................................................................................ ................ . . . . . . . . . . . . . . . . . . . .
(Total for Question 1 = 7 marks)
4
*P79088A0436*
