CLINICAL CHEMISTRY I ASCP UPDATED ACTUAL EXAM QUESTIONS CORRECT ANSWERS GRADED A PLUS

CLINICAL CHEMISTRY I ASCP UPDATED ACTUAL EXAM QUESTIONS CORRECT
ANSWERS GRADED A PLUS
Clinical Chemistry I ASCP Comprehensive TEST
2026/2027 Questions and Answers Verified Solutions
Latest Update

Question:
when measuring thyroxine (T4) by fluorescent polarization immunoassay, which of the following is
true? T4 binds to specific antibody to t4 bound to microtiter plate there is no separation or wash step
fluorescent-labeled drug competes with patient T4 for polarized antibody reaction time is slower
than radioimmunoassay (RIA) but fluorescent polarization immunoassay is more common owing to
safety issues with RIA

Answer:
there is no separation or wash step



Question:
which of the following is reversibly bound to a cellophane membrane impregnated with special
molecules which develops a potential compared to the internal silver electrode? K+, Mg++, Cl,
Ca++

Answer:
Ca++



Question:
a patient has increased serum phosphorus, decreased plasma free calcium, and decreased urinary
phosphorus. this correlates with which of the following?

Answer:
primary hypoparathyroidism



Question:
for therapeutic drug monitoring, how should the specimen be handled?

,Answer:
mixed well to distribute heparin throughout before centrifugation



Question:
in reverse-phase high-performance liquid chromatography for therapeutic drug monitoring, the term
reverse phase implies that the column eluate is which of the following?

Answer:
more polar than the stationary phase



Question:
when analyzing urine specimens for drugs of abuse testing, immunoassay is the method of choice
for screening. what is the method of choice for confirmation?

Answer:
gas chromatography/mass spec



Question:
glycolysis

Answer:
metabolism of glucose molecule to pyruvate or lactate for production of energy



Question:
glycogenesis

Answer:
conversion of glucose to glycogen for storage



Question:
glycogenolysis

,Answer:
breakdown of glycogen to glucose for use as energy



Question:
gluconeogenesis

Answer:
formation of glucose-6-phosphate form non-carbohydrate sources



Question:
lipogenesis

Answer:
conversion of carbohydrates to fatty acids



Question:
lipolysis

Answer:
decomposition of fat



Question:
formation of HbA1c

Answer:
1. glucose reversibly binds to accessible amino groups, forms aldimine (pre-HbA1c) 2. aldimine
undergoes irreversible Amadori rearrangement to form ketoamine (HbA1c) 3. formation of glucose
ring structure



Question:
action of insulin (especially in fed state)

, Answer:
increases glycogenesis and glycolysis: glucose to glycogen to pyruvate to acetyl-CoA increases
lipogenesis decreases glycogenolysis



Question:
action of glucagon (especially in fasting state)

Answer:
increases glycogenolysis- glycogen to glucose increases gluconeogenesis- fatty acids to acetyl-CoA
to ketone, proteins to amino acids



Question:
Hexokinase test

Answer:
reaction is HK/G6P coupled reaction, forming NADPH measure absorbance at 340nm. specimen is
serum or NaF/KOz or EDTA plasma, urine. interference is hemolysis, drugs, bilirubin, lipemia



Question:
glucose oxidase/peroxidase test

Answer:
GOD/PO coupled reaction forming chromogen. specimen is serum or NaF/KOx plasma in SST, RT
<24hr, 2-6C, less than 7 days, -20C for <1yr, random urine for dipstick only, CSF. interference
includes uric acid, ascorbic acid, bilirubin, hemoglobin, tetracycline, glutathione inhibit reaction.



Question:
glucose oxidase/polaro-graphic test

Answer:
glucose + O2-GOD -> H2O2*O2. consumption is measured by polarography. specimen is serum or
NaF/KOx plasma, not whole blood. less interference including with ascorbic acid