UAMS Hematology Lab Practical Final Review| Actual exam questions with correct answers

UAMS Hematology Lab Practical Final Review| Actual exam questions with correct answers What is the smear prep process? Place drop of blood from EDTA tube on with 1/2 inch from the frosted end of a slide. Lay the slide on a flat surface. Place the end of a second (spreader) slide, held with the fingertips at an angle of 30-45 degrees, in front of the drop of blood. Pull the spreader slide back into the drop of blood. Allow blood to spread entire width of slide. Quickly and smoothly push the spreader slide forward. What tools do you need to prepare a smear? Glass slides, blood, cover slip What causes a bad smear? Dirty slide, jerky motion, pointed feather edge, angle of the spreader slide too steep, did not allow blood to spread, high level of lipids, unequal pressure. What is the coverslip smear used for? Used for making bone marrow preps. What the items needed for the Wright's stain? Wright's stain, buffer, rinse solution, timer, jars or other containers for holding the stain, buffer, and rinse. What are the sources of error when staining a smear? Stain or buffer is too acidic or alkaline, prolonged staining, heparinized blood sample, prolonged rinsing, under-buffering. What is a good area when viewing a blood smear? Cells should be touching each other with minimal overlap. How do you perform a WBC estimate? Select a good area on slide. Average the number of white cells in five low power fields. Multiply the average by 200. (One white cell under low power, 10x, equals approximately 200 WBCs) How do you check for platelet clumps? Check for platelet clumps after performing WBC estimate. Look at the feather edge or side margins of the smear. If you can't get your slide to focus, what are the potential issues?